scholarly journals Three Abietane Diterpenes and Two Diterpenes Incorporated Sesquiterpenes from the Bark of Cryptomeria japonica

2006 ◽  
Vol 54 (3) ◽  
pp. 315-319 ◽  
Author(s):  
Kazuko Yoshikawa ◽  
Toshinori Tanaka ◽  
Akemi Umeyama ◽  
Shigenobu Arihara
Keyword(s):  
Cryptomeria Japonica ◽  
Abietane Diterpenes

Three Abietane Diterpenes and Two Diterpenes Incorporated Sesquiterpenes from the Bark of Cryptomeria japonica.

ChemInform ◽  
2006 ◽  
Vol 37 (34) ◽  
Author(s):  
Kazuko Yoshikawa ◽  
Toshinori Tanaka ◽  
Akemi Umeyama ◽  
Shigenobu Arihara
Keyword(s):  
Cryptomeria Japonica ◽  
Abietane Diterpenes

scholarly journals A New Abietane and Two Dimeric Abietane Diterpenes from the Black Heartwood of Cryptomeria japonica

2004 ◽  
Vol 52 (3) ◽  
pp. 354-358 ◽  
Author(s):  
Shigenobu Arihara ◽  
Akemi Umeyama ◽  
Shinya Bando ◽  
Shinji Imoto ◽  
Mikako Ono ◽  
...  
Keyword(s):  
Cryptomeria Japonica ◽  
Abietane Diterpenes ◽  
Black Heartwood

A New Abietane and Two Dimeric Abietane Diterpenes from the Black Heartwood of Cryptomeria japonica.

ChemInform ◽  
2004 ◽  
Vol 35 (34) ◽  
Author(s):  
Shigenobu Arihara ◽  
Akemi Umeyama ◽  
Shinya Bando ◽  
Shinji Imoto ◽  
Mikako Ono ◽  
...  
Keyword(s):  
Cryptomeria Japonica ◽  
Abietane Diterpenes ◽  
Black Heartwood

Abietane diterpenes from Plectranthus madagascariensis: A cytotoxicity screening

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
D Matias ◽  
F Pereira ◽  
M Nicolai ◽  
A Roberto ◽  
N Saraiva ◽  
...  
Keyword(s):  
Abietane Diterpenes

A New Seco-Abietanoid from the Bark of Cryptomeria japonica

2021 ◽  
Author(s):  
Chi-I Chang ◽  
Cheng-Chi Chen ◽  
Yueh-Hsiung Kuo
Keyword(s):  
Cryptomeria Japonica

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cryptomeria Japonica ◽  
Fluorescent Protein ◽  
Japanese Cedar ◽  
Targeted Mutagenesis ◽  
Embryogenic Tissue ◽  
Breeding Period ◽  
Single Mutation ◽  
Knock Out ◽  
Guide Rna

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.

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