scholarly journals Fluorimetric Determination of Ascorbic Acid in Vitamin C Tablets Using Methylene Blue

2005 ◽  
Vol 53 (10) ◽  
pp. 1251-1254 ◽  
Author(s):  
Yusuf Dilgin ◽  
Gürel Nişli
2012 ◽  
Vol 23 (2) ◽  
pp. 174-179 ◽  
Author(s):  
Nicoleta Matei ◽  
Simona Dobrinas ◽  
Gabriel Lucian Radu

AbstractThe objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes (Vitis vinifera L.) just previous ingathering. In the present work was used the red and white grapes from Murfatlar vineyard: Mamaia, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon, Muscat Ottonel and Riesling Italian. The results were situated in the range of 0.67 - 1.79 mg vitamin C/100g product for red grapes and respectively 0.50 - 1.49 mg vitamin C/100g for white grapes.


2017 ◽  
Vol 3 (1) ◽  
pp. 19-22
Author(s):  
Fabian Audu Ugbe ◽  
Victoria Abiola Ikudayisi ◽  
Oluwagbemiga Tayo Amusan

This research was aimed at investigating the concentration of Ascorbic Acid (Vitamin C) in some commercial fruits juices: ‘5’ Alive, Hollandia yoghurt, Nutri milk, Ribena, Lacasera, Happy hour, Viju milk, Chi exotic and Chi vita sold in Ugbokolo, Benue State, Nigeria by titrimetric method using 2,6-dichlorophenolindophenol (DCPIP) as titrant. The ascorbic acid (AA) content in milligram per 100ml of the various juices investigated is in the order: ‘5’ Alive (11.25) > Ribena (5.47) > nutri milk (5.31) > Happy hour (4.38) > Hollandia yoghurt (1.25) > Lacasera (0.47) ≈ Chi exotic (0.47) >Viju milk (0.31) > Chi vita (0.16). It was observed that half a litre of ‘5’ Alive (56.25 mg AA) a day is suitable for adults and pregnant women, a liter each of Ribena  (54.70 mg AA) and Nutri milk (53.10 mg AA) per day for adults, while a litre of Happy hour (43.80 mg AA) per day is suitable for adolescents. It can be concluded that commercial fruits juices when taken in large amount will add to the daily human dietary intake of the vitamin.


2008 ◽  
Vol 24 (6) ◽  
pp. 721-725 ◽  
Author(s):  
Abdolkarim ABBASPOUR ◽  
Abdolreza KHAJEHZADEH ◽  
Abolhassan NOORI

1989 ◽  
Vol 72 (4) ◽  
pp. 681-686
Author(s):  
Hie-Joon Kim

Abstract A rapid and sensitive liquid chromatographic method for determination of total vitamin C in foods and beverages is described. Ascorbic acid and dehydroascorbic acid are extracted with sulfuric acid solution, and the dehydroascorbic acid in the extract is reduced to ascorbic acid by dithiothreitol at pH 7. The reduction is complete in 2 min at room temperature. The resulting total ascorbic acid is separated on an anion exclusion/high speed column with 20mM sulfuric acid as eluant and detected amperometrically with a platinum electrode operating at +0.6-0.8 V vs Ag/AgCl reference electrode. Dithiothreitol (retention time, 3.2 min) does not interfere with the separation and detection of ascorbic acid (retention time, 1.3 min). The dehydroascorbic acid content can be estimated as the difference in ascorbic acid content measured with and without reduction by dithiothreitol. The completeness of the reduction was demonstrated by purposely allowing the oxidation of ascorbic acid in the food extract and determining the total vitamin C after reduction. The determinations of vitamin C content in selected foods and beverages were in good agreement with the expected values. Total analysis time for vitamin C is 10 min and the detection limit is 0.1 ng. The method is specific for vitamin C, and interference by other food constituents is minimal.


1976 ◽  
Vol 59 (6) ◽  
pp. 1244-1250 ◽  
Author(s):  
Ram B Roy ◽  
Aldo Conetta ◽  
Jerry Salpeter

Abstract A specific microfluorometric method for the determination of ascorbic acid, dehydroascorbic acid, and total vitamin C in food products has been automated. The procedure developed is an adaptation of the official AOAC method (secs. 43.056–43.062), except that N-bromosuccinimide is used instead of Norit to oxidize vitamin C. Ascorbic acid is selectively oxidized by N-bromosuccinimide before other interfering substances that may be present, so this method is a highly sensitive and specific technique with extensive applicability. The proposed automated method is simple, rapid, reliable, and sufficiently sensitive to analyze as little as 2 × 10−3 to 0.1 mg ascorbic acid/ml. Analytical results obtained for ascorbic acid, dehydroascorbic acid, and total vitamin C in a wide variety of food products are reported. The analytical system developed has the capability of analyzing 50 samples/hr.


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