scholarly journals Purification and Characterization of Ginsenoside-.BETA.-Glucosidase from Ginseng.

2001 ◽  
Vol 49 (7) ◽  
pp. 795-798 ◽  
Author(s):  
Chunzhi ZHANG ◽  
Hongshan YU ◽  
Yongming BAO ◽  
Lijia AN ◽  
Fengxie JIN
2001 ◽  
Vol 49 (9) ◽  
pp. 4457-4462 ◽  
Author(s):  
Randall G. Cameron ◽  
John A. Manthey ◽  
Robert A. Baker ◽  
Karel Grohmann

1980 ◽  
Vol 185 (2) ◽  
pp. 515-519 ◽  
Author(s):  
M Inglin ◽  
B A Feinberg ◽  
J R Loewenberg

A new intracellular beta-glucosidase was isolated from Trichoderma reesei. It was sequentially purified by (NH4)2SO4 precipitation and chromatography and rechromatography on Sephadex G-150. The enzyme has a mol.wt. of 98 000, optimal activity at pH 6.5, pI 4.4 and Km values of 6.7 mM and 3.3 mM for sophorose and cellobiose respectively. Possible functions of the enzyme may be regulation of cellulase induction and/or to serve as a proenzyme.


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