scholarly journals Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia.

1989 ◽  
Vol 37 (8) ◽  
pp. 2179-2182 ◽  
Author(s):  
Masatoshi YAMAZAKI ◽  
Shigeru TANSHO ◽  
Jun KISUGI ◽  
Koji MURAMOTO ◽  
Hisao KAMIYA
Keyword(s):  
Purification And Characterization ◽  
Sea Hare ◽  
Dolabella Auricularia

Purification and Characterization of a Cellulase from Dolabella auricularia

1984 ◽  
Vol 96 (5) ◽  
pp. 1381-1390 ◽  
Author(s):  
Hirosi ANZAI ◽  
Kazutosi NISIZAWA ◽  
Kazuo MATSUDA
Keyword(s):  
Purification And Characterization ◽  
Dolabella Auricularia

Purification and characterization of an antibacterial and antineoplastic protein secretion of a sea hare, Aplysia juliana

Toxicon ◽  
1989 ◽  
Vol 27 (12) ◽  
pp. 1269-1277 ◽  
Author(s):  
Hisao Kamiya ◽  
Koji Muramoto ◽  
Rina Goto ◽  
Masahiro Sakai ◽  
Yoshio Endo ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Purification And Characterization ◽  
Sea Hare

Purification and characterization of phosphoglucomutase from peas

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger
Keyword(s):  
Purification And Characterization

Purification and Characterization of Tissue-Type Plasminogen Activator in Maxillary Mucosa with Chronic Inflammation

1985 ◽  
Vol 54 (02) ◽  
pp. 485-489 ◽  
Author(s):  
Yukiyoshi Hamaguchi ◽  
Masuichi Ohi ◽  
Yasuo Sakakura ◽  
Yasuro Miyoshi
Keyword(s):  
Plasminogen Activator ◽  
Chronic Inflammation ◽  
Gel Filtration ◽  
Potassium Acetate ◽  
Tissue Type ◽  
Purification And Characterization ◽  
Tissue Type Plasminogen Activator ◽  
Urokinase Inhibitor ◽  
Type Plasminogen Activator

SummaryTissue-type plasminogen activator (TPA) was purified from maxillary mucosa with chronic inflammation and compared with urokinase. Purification procedure consisted of the extraction from delipidated mucosa with 0.3M potassium acetate buffer (pH 4.2), 66% saturation of ammonium sulfate, zinc chelate-Sepharose, concanavalin A-Sepharose and Sephadex G-100 gel filtration chromatographies.The molecular weight of the TPA was approximately 58,000 ± 3,000. Its activity was enhanced in the presence of fibrin and was quenched by placental urokinase inhibitor, but not quenched by anti-urokinase antibody. The TPA made no precipitin line against anti-urokinase antibody, while urokinase did.All these findings indicate that the TPA in maxillary mucosa with chronic inflammation is immunologically dissimilar to urokinase and in its affinity for fibrin.

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