scholarly journals Purification and characterization of type IV collagen from mouse kidney.

1986 ◽  
Vol 34 (2) ◽  
pp. 789-797 ◽  
Author(s):  
TSUTOMU OIKAWA ◽  
TAKAO IWAGUCHI ◽  
MIKIO KIMURA ◽  
AKIO MATSUZAWA
Keyword(s):  
Type Iv Collagen ◽  
Mouse Kidney ◽  
Purification And Characterization ◽  
Type Iv

Partial purification and characterization of a neutral protease which cleaves type IV collagen

Biochemistry ◽  
1981 ◽  
Vol 20 (1) ◽  
pp. 100-104 ◽  
Author(s):  
Lance A. Liotta ◽  
Karl Tryggvason ◽  
Spiridione Garbisa ◽  
Pamela Gehron Robey ◽  
Shigeto Abe
Keyword(s):  
Type Iv Collagen ◽  
Neutral Protease ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Type Iv

Isolation and characterization of pepsin-solubilized human basement membrane (type IV) collagen peptides

Biochemistry ◽  
1983 ◽  
Vol 22 (21) ◽  
pp. 4940-4948 ◽  
Author(s):  
Robert S. MacWright ◽  
Virginia A. Benson ◽  
Katherine T. Lovello ◽  
Michel Van der Rest ◽  
Peter P. Fietzek
Keyword(s):  
Basement Membrane ◽  
Type Iv Collagen ◽  
Collagen Peptides ◽  
Type Iv ◽  
Isolation And Characterization ◽  
Membrane Type

viking: identification and characterization of a second type IV collagen in Drosophila

Gene ◽  
1997 ◽  
Vol 198 (1-2) ◽  
pp. 17-25 ◽  
Author(s):  
Sukkid Yasothornsrikul ◽  
Wendy J Davis ◽  
Gabrielle Cramer ◽  
Deborah A Kimbrell ◽  
Charles R Dearolf
Keyword(s):  
Type Iv Collagen ◽  
Type Iv ◽  
Identification And Characterization

scholarly journals Purification and characterization of histidine decarboxylase from mouse kidney

1986 ◽  
Vol 234 (2) ◽  
pp. 349-354 ◽  
Author(s):  
S A M Martin ◽  
J O Bishop
Keyword(s):  
Column Chromatography ◽  
Gel Electrophoresis ◽  
Histidine Decarboxylase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Mouse Kidney ◽  
Purification Procedure ◽  
Purification And Characterization ◽  
A Subunit

Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative polyacrylamide-gel electrophoresis. The native enzyme had an estimated Mr of 113 000. The protein was analysed in SDS/10%-polyacrylamide gels and formed a single band corresponding to a subunit Mr of 55 000, indicating that it is a dimer. Three forms of the enzyme were resolved on isoelectrofocusing gels, with pI 5.3, 5.5 and 5.7.

Partial characterization of the heteropolysaccharide associated with the 7S domain of type IV collagen from placenta

1987 ◽  
Vol 65 (5) ◽  
pp. 501-506 ◽  
Author(s):  
James R. A. Leushner
Keyword(s):  
Sialic Acid ◽  
Amino Acid ◽  
Chemical Analysis ◽  
Amino Acid Analysis ◽  
Molecular Sieve ◽  
Type Iv Collagen ◽  
Relative Mass ◽  
Type Iv ◽  
Structure Formula

A major heteropolysaccharide fraction was isolated from the 7S domain of human placental type IV collagen. Analyses revealed that it was an asparagine-linked oligosaccharide. Characterization using molecular sieve chromatography, exoglycosidase and endoglycosidase digestion, and chemical analysis suggested a bianternnary complex with the following structure:[Formula: see text]A microheterogeneity was noted with respect to the addition of the fucose and sialic acid residues. Analysis of component polypeptides of the 7S fraction following endoglycosidase treatment suggested that the most obvious site of heteropolysaccharide attachment was in a polypeptide of relative mass 40 000. Amino acid analysis of this isolated polypeptide indicated that it was rich in collagenous sequences and also contained half-cystine residues.

scholarly journals Preparation and partial characterization of monoclonal antibodies specific for the nascent non-triple helical form of the type IV collagen alpha 1 chain

2017 ◽  
Vol 9 ◽  
pp. 128-132 ◽  
Author(s):  
Makoto Morita ◽  
Hidemitsu Sugihara ◽  
Kazuhiro Tokunaka ◽  
Arihiro Tomura ◽  
Kan Saiga ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Type Iv Collagen ◽  
Partial Characterization ◽  
Type Iv
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