scholarly journals Differential determination of human liver and pancreas dipeptidase activities by using specific antibody-conjugated paper disks.

1986 ◽  
Vol 34 (3) ◽  
pp. 1176-1183 ◽  
Author(s):  
YOSHIMASA ITO ◽  
MASAHIRO IWAKI ◽  
TARO OGISO ◽  
KAZUYUKI HIRANO ◽  
MAMORU SUGIURA ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Human Liver ◽  
Liver And Pancreas ◽  
Dipeptidase Activities

scholarly journals Characterization of antibody against human liver guanase by immunoblotting and immunohistochemical staining of human liver guanase by a direct labeling antibody-enzyme method.

1989 ◽  
Vol 37 (5) ◽  
pp. 611-615 ◽  
Author(s):  
S Ito ◽  
A Iwasaki ◽  
J Syundo ◽  
Y Tamura ◽  
S Kishi ◽  
...  
Keyword(s):  
Liver Enzyme ◽  
Specific Antibody ◽  
Immunohistochemical Staining ◽  
Human Liver ◽  
Liver Extract ◽  
Precipitin Line ◽  
Tissue Sections ◽  
Immunohistochemical Reaction ◽  
Enzyme Method

Human liver guanase was purified and a specific antibody against it was raised in rabbits. The antiserum formed a single precipitin line with human liver extract, and also completely inhibited the activity of the liver enzyme. An immunoblotting study showed that the antibody bound specifically to one band of protein with guanase activity and not to other proteins. Therefore, we concluded that this antiserum against the liver enzyme was suitable for use in immunohistochemical demonstration of guanase. In tissue sections, the immunohistochemical reaction with this antibody was positive in the same locations as the histochemical guanase reaction with DAB (3,3'-diaminobenzidine tetrahydrochloride).

scholarly journals FREQUENCY OF DETERMINATION OF PARENCHYMAL ORGANS DISTURBANCES DURING ULTRASOUND EXAMINATION OF REPRODUCTIVE TRACT

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Strahinja ĆIBIĆ ◽  
Miloš PAVLOVIĆ
Keyword(s):  
Genital Tract ◽  
Reproductive Tract ◽  
Ultrasound Examination ◽  
Non Invasive ◽  
Follicular Cyst ◽  
Pathological Conditions ◽  
Ultrasound Diagnostics ◽  
Liver And Pancreas ◽  
Invasive Method

Ultrasound Real time 2D diagnostics, being an available and non-invasive method, issuccessfully used in human medicine, veterinary medicine and biology to diagnose bothphysiological and pathological conditions. In the case of small animals, the ultrasound is often usedto diagnose gravidity, number, size and vitality of fetuses as well as to diagnose pathologicalconditions present in the genital tract, and is also used to examine testicles and prostate of maleanimals. Today's advanced use of the ultrasound diagnostics provides us with an opportunity todetect numerous pathological conditions of reproductive tract of both female and male animals, andthose conditions directly affect health status of other internal organs. The aim of this study is todiagnose primary genital tract disorders using the ultrasound diagnostics, as well as proving itsconnection to the changes in parenchymal organs. The ultrasound examination was performed onthe total of 12 dogs, 6 male and 6 female dogs. Ovaries, uterus and the organs in the abdominal areawere examined using a transducer ranging from 5 to 8 MHz. A transducer of 7.5 MHz was used toexamine prostates and testicles. The patients were in dorsal position. Three out of six examined maledogs exhibited changes on their prostates, and the remaining one had a tumor on the testicles. Cystitisand hyperplasia of prostate were also found upon the examination of these dogs. One out of sixexamined female dogs, had cystic endometrial hyperplasia, three of them had pyometra, and one ofthem was diagnosed with an follicular cyst. In addition to these pathological conditions, changeswere noticed in the liver and pancreas. Along with the detection of primary changes in the genitaltract of both male and female animals, the ultrasound diagnostics can be used to discover consequentchanges in parenchymal organs.

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