scholarly journals An ultramicro fluorimetric determination of total ascorbic acid in human serum using 1,2-diamino-4,5-dimethoxybenzene.

1985 ◽  
Vol 33 (8) ◽  
pp. 3499-3502 ◽  
Author(s):  
TETSUHARU IWATA ◽  
SHUUJI HARA ◽  
MASATOSHI YAMAGUCHI ◽  
MASARU NAKAMURA ◽  
YOSUKE OHKURA
Keyword(s):  
Ascorbic Acid ◽  
Human Serum ◽  
Fluorimetric Determination

Kinetic fluorimetric determination of the antineoplastic methotrexate (MTX) in human serum

2002 ◽  
Vol 29 (5) ◽  
pp. 851-858 ◽  
Author(s):  
A. Espinosa-Mansilla ◽  
I. Durán Merás ◽  
A. Zamora Madera ◽  
L. Pedano ◽  
C. Ferreyra
Keyword(s):  
Human Serum ◽  
Fluorimetric Determination

Flow injection Fluorimetric Determination of Ascorbic Acid Based on its Photooxidation by Thionine Blue

The Analyst ◽  
1997 ◽  
Vol 122 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Tomás Pérez-Ruiz ◽  
Carmen Martínez-Lozano ◽  
Virginia Tomás ◽  
Ciriaco Sidrach
Keyword(s):  
Ascorbic Acid ◽  
Flow Injection ◽  
Fluorimetric Determination

Fluorimetric determination of total ascorbic acid by a stopped-flow mixing technique

The Analyst ◽  
2001 ◽  
Vol 126 (8) ◽  
pp. 1436-1439 ◽  
Author(s):  
Tomás Pérez-Ruiz ◽  
Carmen Martínez-Lozano ◽  
Virginia Tomás ◽  
José Fenol
Keyword(s):  
Ascorbic Acid ◽  
Stopped Flow ◽  
Fluorimetric Determination ◽  
Flow Mixing

Fluorimetric determination of albumin and globulin in human serum using tetra-substituted sulphonated aluminum phthalocyanine

1999 ◽  
Vol 401 (1-2) ◽  
pp. 185-189 ◽  
Author(s):  
Dong-Hui Li ◽  
Huang-Hao Yang ◽  
Hong Zhen ◽  
Ying Fang ◽  
Qing-Zhi Zhu ◽  
...  
Keyword(s):  
Human Serum ◽  
Fluorimetric Determination ◽  
Aluminum Phthalocyanine

scholarly journals Protein-templated copper nanoclusters for fluorimetric determination of human serum albumin

2021 ◽  
Vol 188 (4) ◽  
Author(s):  
Mariagrazia Lettieri ◽  
Pasquale Palladino ◽  
Simona Scarano ◽  
Maria Minunni
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescent Detection ◽  
Fluorimetric Determination ◽  
Copper Nanoclusters ◽  
Optical Reporters ◽  
The Stability ◽  
Serum And Urine

AbstractCopper nanoclusters (CuNCs) are attractive for their unique optical properties, providing sensitive fluorescent detection of several kinds of targets even in complex matrices. Their ability in growing on suitable protein and nucleic acid templates make CuNCs efficient optical reporters to be exploited in bioanalysis. In this work, we report the specific and sensitive determination of human serum albumin (HSA) in human serum (HS) and urine via CuNCs fluorescence. HSA is the most abundant protein in plasma, and plays a key role in the early diagnosis of serious pathological conditions such as albuminuria and albuminemia. Recently, HSA has become clinically central also as a biomarker to assess severity, progression, and prognosis of various cancers. We report the controlled and reproducible growth of CuNCs directly on the target analyte, HSA, which results in a fine dose-dependent fluorescent emission at 405 nm. The protocol is optimized in water, and then applied to serum and urine specimens, without matrix pretreatment. The method linearly responds within the whole concentration of clinical interest, with a sensitivity of 1.8 ± 0.1 × 10−3 g L−1 and 0.62 ± 0.03 × 10−3 g L−1 in serum and urine, respectively, and excellent reproducibility (CVav% ca. 3% for both). The assay is designed to have a single protocol working for both matrices, with recovery of 95% (HS) and 96% (urine). The stability of the fluorescence after CuNCs formation was tested over 3 days, displaying good results (yet higher in urine than in serum). Graphical abstract

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