scholarly journals Effect of extract from Paeoniae Radix on urea-nitrogen concentration in rat serum. I.

1981 ◽  
Vol 29 (3) ◽  
pp. 874-878 ◽  
Author(s):  
SHINYA SHIBUTANI ◽  
TETSURO NAGASAWA ◽  
HIKOKICHI OURA ◽  
GENICHIRO NONAKA ◽  
ITSUO NISHIOKA
1980 ◽  
Vol 28 (6) ◽  
pp. 1736-1739 ◽  
Author(s):  
TETSURO NAGASAWA ◽  
SHINYA SHIBUTANI ◽  
HIKOKICHI OURA ◽  
YUKIHIRO SHOYAMA ◽  
ITSUO NISHIOKA

2013 ◽  
Vol 91 (2) ◽  
pp. 811-818 ◽  
Author(s):  
V. V. Almeida ◽  
A. J. C. Nuñez ◽  
A. P. Schinckel ◽  
C. Andrade ◽  
J. C. C. Balieiro ◽  
...  

1981 ◽  
Vol 27 (3) ◽  
pp. 480-485 ◽  
Author(s):  
Z L Bandi ◽  
J B Fuller ◽  
D E Bee ◽  
G P James

Abstract We followed the "abbreviated precision protocol" of the National Committee for Clinical Laboratory Standards for the evaluation of precision, accuracy, and carryover in analysis for urea nitrogen with the multilayer film analysis system ("Ektachem"). We analyzed 456 clinical samples with this instrument, by the manual urease/glucose dehydrogenase method, and with the Beckman System I GLU/BUN Analyzer. Precision and accuracy were estimated for 50, 220, 270, and 500 mg/L urea nitrogen concentrations in 100, 30, or 20 microL of serum. Potential interference of 15 compounds was evaluated. Random error (defined as 1.965 X SD) was 7, 10, 12, and 18 mg/L. Systematic error was 3, 4, 5, and 15 mg/L. Total analytical error was 11, 14, 17, and 34 mg/L for analysis of 100 microL of serum at the above-stated urea nitrogen concentrations. The greatest interference (6 mg/L) was caused by ethanol (300 mg/L) and by hemoglobin (500 mg/L) in the urea nitrogen (at 260 mg/L) determination. Urea nitrogen concentration, as determined with the Ektachem was linearly related to the expected concentration, at least up to 1187 mg/L. Carryover was not statistically significant.


2008 ◽  
Vol 33 (1) ◽  
pp. 7-12 ◽  
Author(s):  
J. L. Figueroa ◽  
M. Martínez ◽  
J. E. Trujillo ◽  
V. Zamora ◽  
J. L. Cordero ◽  
...  

2007 ◽  
Vol 292 (1) ◽  
pp. F467-F474 ◽  
Author(s):  
David A. Spector ◽  
Qing Yang ◽  
James B. Wade

Although the mammalian urinary tract is generally held to be solely a transit and storage vehicle for urine made by the kidney, in vivo data suggest reabsorption of urea and other urine constituents across urinary tract epithelia. To determine whether urinary tract tissue concentrations are increased as a result of such reabsorption, we measured urea nitrogen and creatinine concentrations and determined whether urea transporter B (UT-B) was present in bladder, ureter, and other tissues from dogs and rats. Mean urea nitrogen and creatinine concentrations in dogs and rats were three- to sevenfold higher in urinary tract tissues than in serum and were comparable to those in renal cortex. In water-restricted or water-loaded rats, urea nitrogen concentrations in bladder tissues fell inversely with the state of hydration, were proportional to urine urea nitrogen concentrations, and were greater than the corresponding serum urea nitrogen concentration in every animal. Immunoblots of rat and dog urinary tract tissues demonstrated the presence of UT-B in homogenates of bladder and ureter, and immunocytochemical analysis localized UT-B to epithelial cell membranes. These findings are consistent with the notion that urea and creatinine are continuously reabsorbed from the urine across the urothelium, urea in part via UT-B, and that urine is thus altered in its passage through the urinary tract. Urea reabsorption across urinary tract epithelia may be important during conditions requiring nitrogen conservation and may contribute to pathophysiological states characterized by high blood urea nitrogen, such as prerenal azotemia and obstructive uropathy.


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