scholarly journals In vitro studies on the metabolic pathway of SQ 14225 (captopril) and mechanism of mixed disulfide formation.

1981 ◽  
Vol 4 (9) ◽  
pp. 677-684 ◽  
Author(s):  
TORU KOMAI ◽  
TOSHIHIKO IKEDA ◽  
KENJI KAWAI ◽  
EMI KAMEYAMA ◽  
HIDEYO SHINDO
Keyword(s):  
Metabolic Pathway ◽  
In Vitro Studies ◽  
Disulfide Formation ◽  
Mixed Disulfide

scholarly journals Mixed disulfide formation in vitro between a glycoprotein substrate and yeast oligosaccharyltransferase subunits Ost3p and Ost6p

2013 ◽  
Vol 432 (3) ◽  
pp. 438-443 ◽  
Author(s):  
Siti N.H. Mohd Yusuf ◽  
Ulla-Maja Bailey ◽  
Nikki Y. Tan ◽  
Muhammad Fairuz Jamaluddin ◽  
Benjamin L. Schulz
Keyword(s):  
Disulfide Formation ◽  
Mixed Disulfide

scholarly journals Producción de metabolitos en fibroblastos incubados con acido oleico deuterado./Metabolite production of fibroblasts incubated with deuterated oleic acid

2013 ◽  
Vol 13 (2) ◽  
pp. 202-207
Author(s):  
José Henry Osorio
Keyword(s):  
Mass Spectrometry ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Metabolic Pathway ◽  
In Vitro Studies ◽  
Fatty Acid Degradation ◽  
Metabolic Alterations ◽  
Metabolite Production ◽  
Mitochondrial Fatty Acid

Objetivo: los estudios in vitro, que buscan intermediarios de la degradación mitocondrial de ácidos grasos, facilitan el diagnóstico de alteraciones hereditarias o adquiridas en esa ruta metabólica, el presente estudio analiza la produccion de metabolitos en fibroblastos incubados con acido oleico deuterado. Materiales y métodos: fibroblastos de personas sin antecedentes de alteraciones metabólicas, fueron incubados en presencia de ácido oléico deuterado, y sus metabolitos fueron analizados mediante cromatografía de gases acoplada a espectrometría de masas (GC-MS) Resultados: Se encontró un perfil característico luego de la incubación de este sustrato por fibroblastos. Conclusiones: Este sustrato podría ser usado para realizar estudios in vitro de algunas deficiencias de la β-oxidació mitocondrial de ácidos grasos, mediante la comparación de fibroblastos normales vs fibroblastos de pacientes que presenten deficiciencias de esa vía metabólica. Objective: In vitro studies for searching intermediates of mitochondrial fatty acid degradation,are a tool for diagnosis of hereditary or adquired alterations of the above mentionedmetabolic pathway. The present work analizes the metabolite production in fibroblastsincubated with deuterated oleic acid. Materials and methods: fibroblasts of personswithout antecedents of metabolic alterations, were incubated with deuterated oleic acidand its metabolites were analyzed using gas chromatograpy-mass spectrometry (GCMS).Results. It was found a characteristic profile after incubation of fibroblats with thissubstrate. Conclusion: this substrate could be used to perform in vitro studies of somemitochondrial fatty acid β-oxidación deficiencies, by comparison of normal fibroblastsvs fibroblats of patients who present defiencies of the above named metabolic pathway  

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation
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