scholarly journals Anti-inflammatory Effect of the Water-Soluble Portion of Porcine Placental Extract in Lipopolysaccharide-Stimulated RAW264.7 Murine Macrophage Cells

2018 ◽  
Vol 41 (8) ◽  
pp. 1251-1256 ◽  
Author(s):  
Masahiko Tebakari ◽  
Yuki Daigo ◽  
Hiroki Ishikawa ◽  
Mika Nakamura ◽  
Junichi Kawashima ◽  
...  
Keyword(s):  
Water Soluble ◽  
Murine Macrophage ◽  
Macrophage Cells ◽  
Anti Inflammatory ◽  
Placental Extract ◽  
Inflammatory Effect

Anti-Inflammatory Effect of Ginsenoside Rh2-Mix on Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cells

2018 ◽  
Vol 21 (10) ◽  
pp. 951-960 ◽  
Author(s):  
Delgerzul Baatar ◽  
Muhammad Zubair Siddiqi ◽  
Wan Taek Im ◽  
Nisar Ul Khaliq ◽  
Seong Gu Hwang
Keyword(s):  
Raw 264.7 ◽  
Murine Macrophage ◽  
Ginsenoside Rh2 ◽  
Macrophage Cells ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Anti-inflammatory effect of Ruta graveolens L. in murine macrophage cells

2006 ◽  
Vol 104 (1-2) ◽  
pp. 234-239 ◽  
Author(s):  
S.K. Raghav ◽  
B. Gupta ◽  
C. Agrawal ◽  
K. Goswami ◽  
H.R. Das
Keyword(s):  
Ruta Graveolens ◽  
Murine Macrophage ◽  
Macrophage Cells ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Factors affecting anti-inflammatory effect of chitooligosaccharides in lipopolysaccharides-induced RAW264.7 macrophage cells

2009 ◽  
Vol 19 (23) ◽  
pp. 6655-6658 ◽  
Author(s):  
Sang-Hoon Lee ◽  
Mahinda Senevirathne ◽  
Chang-Bum Ahn ◽  
Se-Kwon Kim ◽  
Jae-Young Je
Keyword(s):  
Factors Affecting ◽  
Macrophage Cells ◽  
Anti Inflammatory ◽  
Raw264.7 Macrophage ◽  
Inflammatory Effect

scholarly journals Pharmacognostic Screening and Anti-inflammatory Investigation of the Methanol extract of stem bark of Blighia unijugata Baker (Sapindaceae)

2020 ◽  
Vol 10 (4) ◽  
pp. 146-152
Author(s):  
Felix N. Osuala ◽  
Uchenna E. Odoh ◽  
VictoriaC. Onuigbo ◽  
Sylvester C. Ohadoma
Keyword(s):  
Methanol Extract ◽  
Lethal Dose ◽  
Numerical Data ◽  
Stem Bark ◽  
Water Soluble ◽  
Herbal Remedies ◽  
Albino Rats ◽  
Standard Drug ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Introduction: Blighia unijugata is a potent medicinal plant extensively employed in traditional herbal remedies for the treatment of various diseases such as fever, fertility, inflammation, hypertension, migraine and treatment of infections due to microorganisms. Aim: This work, investigates the pharmacognostic screening and anti-inflammatory activities of the methanol extract of B. unijugata stem bark. Method: Collection, drying, pulverization, and methanol extraction of the stem bark were done accordingly. The screening of phytochemical constituents and Pharmacognostic numerical data were carried out. The chromatographic analysis was carried out using TLC. The acute toxicity was determined using Lorke’s method. Methanol extract was investigated for anti-inflammatory effect in albino rats using egg-induced hind paw oedema at doses of 200, 400 and 600mg/kg body weight respectively. Result: The macroscopical investigation showed the stem bark outer layer is greyish and, the inner layer is pale reddish brown, disagreeable odour, bitter, Microscopical screening revealed the presence of starch grains, trichomes and sclerenchyma cells. Chemomicroscopic result revealed the presence of lignin, starch, calcium oxalate, cellulose, stone cells. Qualitative phytochemical screening of the powdered bark showed the presence of saponins, steroids, tannins, and resins. Numerical data: moisture content/ weight loss on drying gave value of 7.7%, percentage yield of 6.7%, alcoholic soluble extractive 5.6%, water soluble extractive 6.3%, total ash of 7.1%,  acid insoluble ash 0.57% and water soluble ash 4.56%. The chromatographic screening results were close compared with the standard drug. Toxicity test established the lethal dose of greater than 5000mg/kg. There was a significant inhibition of the edema p>0.05, the presence of various bioactive constituents may have contributed to the anti-inflammatory properties of the plant extract. Conclusion: The result of this study confirms that the barks of B. unijugata have anti-inflammatory effect and justifies the use as traditional treatment of inflammation and pain. Keywords: Blighia unijugata, Inflammation, Inflammatory agents, Anti inflammatory

scholarly journals Curcumin and Curcumol Inhibit NF-κB and TGF-β1/Smads Signaling Pathways in CSE-Treated RAW246.7 Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Ning Li ◽  
Tian-Hao Liu ◽  
Jing-Ze Yu ◽  
Chen-Xi Li ◽  
Yang Liu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Treated Group ◽  
Macrophage Cells ◽  
Injury Model ◽  
Intracellular Ros ◽  
Anti Inflammatory ◽  
Main Components ◽  
Inflammatory Effect

E-Zhu (Curcuma zedoaria) is known as a classical traditional Chinese medicine and widely used in the treatment of cancers, cardiovascular disease, inflammation, and other diseases. Its main components include curcumol and curcumin, which have anti-inflammatory and antifibrosis effects. Here we established an in vitro inflammatory injury model by stimulating RAW246.7 cells with cigarette smoke extract (CSE) and detected the intervention effects of curcumin and curcumol on CSE-treated Raw246.7 macrophage cells to explore whether the two compounds inhibited the expression of inflammatory cytokines by inhibiting the NF-κB signaling pathway. We detected the antifibrosis effects of curcumin and curcumol via TGF-β1/Smads signaling pathways. The model of macrophage damage group was established by CSE stimulation. Curcumol and curcumin were administered to Raw246.7 macrophage cells. The efficacy of curcumol and curcumin was evaluated by comparing the activation of proinflammatory factors, profibrotic factors, and NF-κB and TGF-β1/Smads signaling pathway. In addition, CSE-treated group was employed to detect whether the efficacy of curcumol and curcumin was dependent on the NF-κB signaling via the pretreatment with the inhibitor of NF-κB. Our findings demonstrated that curcumol and curcumin could reduce the release of intracellular ROS from macrophages, inhibit the NF-κB signaling pathway, and downregulate the release of proinflammatory factor. Curcumol and curcumin inhibited the TGF-β1/Smads signaling pathway and downregulated the release of fibrotic factors. Curcumin showed no anti-inflammatory effect in CSE-treated cells after the inhibition of NF-κB. Curcumol and curcumin showed an anti-inflammatory effect by inhibiting the NF-κB signaling pathway.

scholarly journals Anti-Inflammatory Effect of Cherry Extract Loaded in Polymeric Nanoparticles: Relevance of Particle Internalization in Endothelial Cells

Pharmaceutics ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 500 ◽  
Author(s):  
Denise Beconcini ◽  
Francesca Felice ◽  
Ylenia Zambito ◽  
Angela Fabiano ◽  
Anna Maria Piras ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Prunus Avium ◽  
Umbilical Vein ◽  
Water Soluble ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tetrazolium Salt ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Inflammatory Effect

This study aimed at evaluating the anti-inflammatory effect of natural cherry extract (CE), either free or encapsulated in nanoparticles (NPs) based on chitosan derivatives (Ch-der) or poly(lactic-co-glycolic acid) (PLGA), on human umbilical vein endothelial cells (HUVEC). CE from Prunus avium L. was characterized for total polyphenols, flavonoids, and anthocyanins content. CE and CE-loaded NP cytotoxicity and protective effect on lipopolysaccharide (LPS)-stressed HUVEC were tested by water-soluble tetrazolium salt (WST-1) assay. Pro- and anti-inflammatory cytokines (TNF-α, IL-6, IL-10, and PGE2) released by HUVEC were quantified by enzyme-linked immunosorbent assay (ELISA). All NP types were internalized into HUVEC after 2 h incubation and promoted the anti-inflammatory effect of free CE at the concentration of 2 µg gallic acid equivalents (GAE)/mL. CE-loaded Ch-der NPs showed the highest in vitro uptake and anti-inflammatory activity, blunting the secretion of IL-6, TNF-α, and PGE2 cytokines. Moreover, all NPs reduced the production of nitric oxide and NLRP3 inflammasome, and had a stronger anti-inflammatory effect than the major corticosteroid dexamethasone. In particular, the results demonstrate that natural CE protects endothelial cells from inflammatory stress when encapsulated in NPs based on quaternary ammonium chitosan. The CE beneficial effects were directly related with in vitro internalization of CE-loaded NPs.

Sign in / Sign up

Export Citation Format

Share Document