scholarly journals Dietary Diacetylene Falcarindiol Induces Phase 2 Drug-Metabolizing Enzymes and Blocks Carbon Tetrachloride-Induced Hepatotoxicity in Mice through Suppression of Lipid Peroxidation

2011 ◽  
Vol 34 (3) ◽  
pp. 371-378 ◽  
Author(s):  
Tomokazu Ohnuma ◽  
Eisaburo Anan ◽  
Rika Hoashi ◽  
Yuika Takeda ◽  
Takahito Nishiyama ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Drug Metabolizing Enzymes ◽  
Phase 2 ◽  
Metabolizing Enzymes

Effect of eugenol on drug-metabolizing enzymes of carbon tetrachloride-intoxicated rat liver

1995 ◽  
Vol 49 (11) ◽  
pp. 1703-1707 ◽  
Author(s):  
Parasakthy Kumaravelu ◽  
Deepalakshmi P. Dakshinamoorthy ◽  
Shanthi Subramaniam ◽  
Halagowder Devaraj ◽  
Niranjali S. Devaraj
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Drug Metabolizing Enzymes ◽  
Metabolizing Enzymes

Lisosan G, a powder of grain, does not interfer with the drug metabolizing enzymes and has a protective role on carbon tetrachloride-induced hepatotoxicity

2007 ◽  
Vol 29 (8) ◽  
pp. 1155-1159 ◽  
Author(s):  
Vincenzo Longo ◽  
Vera Chirulli ◽  
Pier Giovanni Gervasi ◽  
Simona Nencioni ◽  
Michela Pellegrini
Keyword(s):  
Carbon Tetrachloride ◽  
Protective Role ◽  
Drug Metabolizing Enzymes ◽  
Metabolizing Enzymes

Cryopreserved rat, dog and monkey hepatocytes: measurement of drug metabolizing enzymes in suspensions and cultures

2004 ◽  
Vol 23 (6) ◽  
pp. 307-316 ◽  
Author(s):  
Nicola J Hewitt ◽  
Dietmar Utesch
Keyword(s):  
De Novo ◽  
Rat Hepatocytes ◽  
Cell Suspensions ◽  
Drug Metabolizing Enzymes ◽  
Phase 2 ◽  
Metabolizing Enzymes ◽  
Cells In Culture ◽  
Enzyme Functions ◽  
New Compounds

Metabolism in fresh and cryopreserved (CP) rat, dog and monkey hepatocyte suspensions and cultures was measured using midazolam (CYP3A), tolbutamide (CYP2C), dextromethorphan (CYP2D) and p-nitrophenol (glucuronosyl S-transferases (UGT), sulphotransferases (ST)). CYP3A, CYP2C9, CYP2D6, UGT and ST enzyme functions in fresh and CP rat, dog and monkey hepatocyte suspensions were retained - CP rat hepatocytes lost some CYP2C activity but this was restored by adding NADPH or by placing the cells in culture, suggesting that the enzyme was still functional. Phase 2 activities were equivalent in fresh and CP hepatocyte suspensions. In some cases, incubation conditions increased the rate of metabolism, possibly reflecting de novo cofactor synthesis. However, this effect was substrate and species dependent and was not always the same in fresh and CP cells. CYP3A, CYP2C, CYP2D, UGT and ST activities at 24 hours of culture of rat and monkey hepatocytes were not compromised by cryopreservation. CYP3A, CYP2D but not CYP2C were lower in 24-hour cultures of CP dog hepatocytes than in fresh cells. Despite being lower than fresh cells, UGT activity in dog CP hepatocytes did not decrease from 0 to 24 hours of culture. Speciesspecific metabolism of p-nitrophenol could be demonstrated in both CP cell suspensions and cultures. In conclusion, these data suggest that the enzyme characteristics of fresh and CP hepatocytes from each species and under specific incubation conditions should be considered when carrying out metabolism studies of new compounds.

scholarly journals Effects of Sino-Japanese herbs in the family Labiatae on the hepatic drug metabolizing enzymes and lipid peroxidation in rats.

1993 ◽  
Vol 101 (5) ◽  
pp. 327-336 ◽  
Author(s):  
Sadao NAKAYAMA ◽  
Kuniya KOIZUMI ◽  
Koji IIJIMA ◽  
Makoto MAYANAGI ◽  
Katsuji OGUCHI
Keyword(s):  
Lipid Peroxidation ◽  
Drug Metabolizing Enzymes ◽  
Metabolizing Enzymes ◽  
Hepatic Drug ◽  
The Family
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