scholarly journals Effect of Vanadate on ATP-Induced Increase in Intracellular Calcium Ion Levels in Human Umbilical Vein Endothelial Cells

2010 ◽  
Vol 33 (6) ◽  
pp. 1060-1062 ◽  
Author(s):  
Namie Nejime ◽  
Yukari Tada ◽  
Satomi Kagota ◽  
Yoko Kubota ◽  
Ikuo Shibuichi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Calcium Ion ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Intracellular Calcium Ion ◽  
Umbilical Vein Endothelial Cells

Thrombin-induced ATP release from human umbilical vein endothelial cells

2012 ◽  
Vol 302 (6) ◽  
pp. C915-C923 ◽  
Author(s):  
Stefanie Gödecke ◽  
Claudia Roderigo ◽  
Christine R. Rose ◽  
Bernhard H. Rauch ◽  
Axel Gödecke ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Vascular System ◽  
Degradation Products ◽  
Umbilical Vein ◽  
Atp Release ◽  
State Level ◽  
Receptor Activation ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

ATP and its degradation products play an important role as signaling molecules in the vascular system, and endothelial cells are considered to be an important source of nucleotide release. To investigate the mechanism and physiological significance of endothelial ATP release, we compared different pharmacological stimuli for their ability to evoke ATP release from first passage cultivated human umbilical vein endothelial cells (HUVECs). Agonists known to increase intracellular Ca2+ levels (A23187, histamine, thrombin) induced a stable, non-lytic ATP release. Since thrombin proved to be the most robust and reproducible stimulus, the molecular mechanism of thrombin-mediated ATP release from HUVECs was further investigated. ATP rapidly increased with thrombin (1 U/ml) and reached a steady-state level after 4 min. Loading the cells with BAPTA-AM to capture intracellular calcium suppressed ATP release. The thrombin-specific, protease-activated receptor 1 (PAR-1)-specific agonist peptide TFLLRN (10 μM) fully mimicked thrombin action on ATP release. To identify the nature of the ATP-permeable pathway, we tested various inhibitors of potential ATP channels for their ability to inhibit the thrombin response. Carbenoxolone, an inhibitor of connexin hemichannels and pannexin channels, as well as Gd3+ were highly effective in blocking the thrombin-mediated ATP release. Specifically targeting connexin43 (Cx43) and pannexin1 (Panx1) revealed that reducing Panx1 expression significantly reduced ATP release, while downregulating Cx43 was ineffective. Our study demonstrates that thrombin at physiological concentrations is a potent stimulus of endothelial ATP release involving PAR-1 receptor activation and intracellular calcium mobilization. ATP is released by a carbenoxolone- and Gd3+- sensitive pathway, most likely involving Panx1 channels.

Effect of P2 receptor on the intracellular calcium increase by cancer cells in human umbilical vein endothelial cells

2008 ◽  
Vol 377 (4-6) ◽  
pp. 429-436 ◽  
Author(s):  
Namie Nejime ◽  
Naoko Tanaka ◽  
Ryoko Yoshihara ◽  
Satomi Kagota ◽  
Noriko Yoshikawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Cancer Cells ◽  
Umbilical Vein ◽  
P2 Receptor ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells
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