scholarly journals Over-expression of Senescence Marker Protein-30 Decreases Reactive Oxygen Species in Human Hepatic Carcinoma Hep G2 Cells

2009 ◽  
Vol 32 (10) ◽  
pp. 1645-1648 ◽  
Author(s):  
Setsuko Handa ◽  
Naoki Maruyama ◽  
Akihito Ishigami
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Marker Protein ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Hepatic Carcinoma ◽  
Over Expression ◽  
G2 Cells

6-Dehydrogingerdione Sensitizes Human Hepatoblastoma Hep G2 Cells to TRAIL-Induced Apoptosis via Reactive Oxygen Species-Mediated Increase of DR5

2010 ◽  
Vol 58 (9) ◽  
pp. 5604-5611 ◽  
Author(s):  
Chung-Yi Chen ◽  
Cheng-Jeng Tai ◽  
Jiin-Tsuey Cheng ◽  
Juan-Juan Zheng ◽  
Ying-Zong Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells ◽  
Induced Apoptosis

Regulation of IGF binding protein-1 in Hep G2 cells by cytokines and reactive oxygen species

1999 ◽  
Vol 276 (3) ◽  
pp. G719-G727 ◽  
Author(s):  
Charles H. Lang ◽  
Gerald J. Nystrom ◽  
Robert A. Frost
Keyword(s):  
Reactive Oxygen Species ◽  
Nuclear Translocation ◽  
Binding Protein ◽  
Reactive Oxygen ◽  
Free Radical Scavengers ◽  
Oxygen Species ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Tnf Α ◽  
G2 Cells

The liver is a major site of synthesis for insulin-like growth factor binding protein (IGFBP)-1. Because IGFBP-1 inhibits many anabolic actions of IGF-I, increases in IGFBP-1 may be partly responsible for the decrease in lean body mass observed in catabolic/inflammatory conditions. This study aimed to determine in Hep G2 cells 1) the sensitivity of IGFBP-1 synthesis to treatment with interleukin (IL)-1, tumor necrosis factor-α (TNF-α), and IL-6, 2) the ability of reactive oxygen species (ROS) to enhance IGFBP-1 production, and 3) the role of ROS in mediating cytokine-induced increases in IGFBP-1. Hep G2 cells responded to IL-1β, TNF-α, and IL-6 with maximal 8- to 10-fold increases in IGFBP-1 production. Although the maximal responsiveness of cells treated with TNF-α and IL-6 was 20–30% less than that with IL-1β, cells demonstrated a similar sensitivity to all cytokines (half-maximal responsive dose of ∼10 ng/ml). A low concentration (3 ng/ml) of all three cytokines had an additive effect on IGFBP-1 production. Cytokines also increased IGFBP-1 mRNA. The half-life of IGFBP-1 mRNA was ∼4 h and not altered by IL-1β. Incubation with ROS, including H2O2and nitric oxide (NO) donors, resulted in a relatively smaller increase in IGFBP-1. However, preincubating Hep G2 cells with various free radical scavengers and NO synthase and eicosanoid inhibitors failed to prevent or attenuate cytokine-induced increases in IGFBP-1. Finally, preincubating cells with pyrrolidinedithiocarbamate (PDTC) but not SN50 (inhibitors of nuclear factor-κB activation and nuclear translocation, respectively) attenuated increases in IGFBP-1 induced by IL-1. These results indicate that 1) proinflammatory cytokines directly enhance IGFBP-1 synthesis by stimulating transcription without altering mRNA stability, 2) addition of exogenous ROS also stimulates IGFBP-1 production but to a smaller extent than cytokines, and 3) the cytokine-induced increase in IGFBP-1 production is not mediated by endogenous production of ROS or eicosanoids but appears to at least partially involve a PDTC-sensitive pathway.

scholarly journals Senescence Marker Protein-30 (SMP30) Deficiency Impairs Myocardium-Induced Dilation of Coronary Arterioles Associated with Reactive Oxygen Species

2013 ◽  
Vol 14 (5) ◽  
pp. 9408-9423 ◽  
Author(s):  
Hiroyuki Mizukami ◽  
Shu-ichi Saitoh ◽  
Hirofumi Machii ◽  
Shinya Yamada ◽  
Yasuto Hoshino ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Marker Protein ◽  
Coronary Arterioles

Senescence marker protein-30 (SMP30) induces formation of microvilli and bile canaliculi in Hep G2 cells

2005 ◽  
Vol 320 (2) ◽  
pp. 243-249 ◽  
Author(s):  
Akihito Ishigami ◽  
Toshiko Fujita ◽  
Haruhiko Inoue ◽  
Setsuko Handa ◽  
Sachiho Kubo ◽  
...  
Keyword(s):  
Marker Protein ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Bile Canaliculi ◽  
G2 Cells

Senescence Marker Protein-30 (SMP30) Rescues Cell Death by Enhancing Plasma Membrane Ca2+-Pumping Activity in Hep G2 Cells

1998 ◽  
Vol 250 (2) ◽  
pp. 374-380 ◽  
Author(s):  
Toshiko Fujita ◽  
Haruhiko Inoue ◽  
Tsuneo Kitamura ◽  
Nobuhiro Sato ◽  
Tastuo Shimosawa ◽  
...  
Keyword(s):  
Cell Death ◽  
Plasma Membrane ◽  
Marker Protein ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells ◽  
Pumping Activity

Senescence marker protein-30 regulates Akt activity and contributes to cell survival in Hep G2 cells

2004 ◽  
Vol 321 (2) ◽  
pp. 386-390 ◽  
Author(s):  
Syujirou Matsuyama ◽  
Tsuneo Kitamura ◽  
Nobuyuki Enomoto ◽  
Toshiko Fujita ◽  
Akihito Ishigami ◽  
...  
Keyword(s):  
Cell Survival ◽  
Marker Protein ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells
Sign in / Sign up

Export Citation Format

Share Document