scholarly journals Effect of Tremella fuciformis on the Neurite Outgrowth of PC12h Cells and the Improvement of Memory in Rats

2007 ◽  
Vol 30 (4) ◽  
pp. 708-714 ◽  
Author(s):  
Ji Hyun Kim ◽  
Hyo-Cheol Ha ◽  
Min-Sook Lee ◽  
Jung-Il Kang ◽  
Hyun-Su Kim ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Tremella Fuciformis ◽  
Pc12h Cells

Role of Nrf2 and p62/ZIP in the neurite outgrowth by carnosic acid in PC12h cells

2009 ◽  
Vol 147 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Kunio Kosaka ◽  
Junsei Mimura ◽  
Ken Itoh ◽  
Takumi Satoh ◽  
Yosuke Shimojo ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Carnosic Acid ◽  
Pc12h Cells

scholarly journals Antisense suppression of TSC1 gene product, hamartin, enhances neurite outgrowth in NGF-treated PC12h cells

2007 ◽  
Vol 29 (8) ◽  
pp. 502-509 ◽  
Author(s):  
Florin Floricel ◽  
Katsumi Higaki ◽  
Hirotoshi Maki ◽  
Eiji Nanba ◽  
Haruaki Ninomiya ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Gene Product ◽  
Antisense Suppression ◽  
Tsc1 Gene ◽  
Pc12h Cells

Staurosporine-induced neurite outgrowth in PC12h cells

1989 ◽  
Vol 184 (2) ◽  
pp. 351-359 ◽  
Author(s):  
Seiichi Hashimoto ◽  
Akihiko Hagino
Keyword(s):  
Neurite Outgrowth ◽  
Pc12h Cells

scholarly journals K-252a, a potent protein kinase inhibitor, blocks nerve growth factor-induced neurite outgrowth and changes in the phosphorylation of proteins in PC12h cells.

1988 ◽  
Vol 107 (4) ◽  
pp. 1531-1539 ◽  
Author(s):  
S Hashimoto
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Neurite Outgrowth ◽  
Neuronal Differentiation ◽  
Actinomycin D ◽  
Kinase Inhibitor ◽  
Protein Kinase Inhibitor ◽  
Nerve Growth ◽  
Pc12h Cells

Nerve growth factor (NGF) promotes neuronal differentiation of PC12 pheochromocytoma cells. One of the most prominent and distinguishing features of neuronal differentiation is neurite outgrowth. The mechanism by which NGF causes the cells to elaborate neurites is unknown. This study shows that K-252a, a potent protein kinase inhibitor, blocks NGF-induced neurite outgrowth and the changes in protein phosphorylation elicited by NGF. In the experiment with intact cells phosphorylated with 32P-orthophosphoric acid, an exposure of PC12h cells to NGF (50 ng/ml) caused an increase in the phosphorylation of tyrosine hydroxylase and a 35,000-D protein and a decrease in a 36,500-D protein. Pretreatment of PC12h cells with K-252a (100 nM) inhibited the effects of NGF on the phosphorylation of these three proteins. In the phosphorylation of cell-free extracts with [gamma-32P] ATP, treatment of PC12h cells with NGF (50 ng/ml) caused a decrease in the phosphorylation of Nsp100. Pretreatment of the cells with K-252a (30 nM) almost completely blocked the NGF effect on the phosphorylation of Nsp100 elicited by subsequent treatment of the cells with NGF. Treatment of PC12h cells with NGF promoted outgrowth of neurites. The addition of K-252a (100 nM) into the culture almost completely blocked the generation of neurites elicited by NGF. Earlier studies demonstrated that NGF-induced neurite outgrowth in PC12 cells involves at least two components: the first of these is transcription-dependent and the second is transcription-independent. To determine the component on which K-252a acts, experiments were carried out on NGF-induced priming or regeneration of neurites. When K-252a was present in the priming step, NGF induced only actinomycin D-sensitive neurites, showing that K-252a interferes with the transcription-dependent actions of NGF. When already primed cells were treated with NGF, actinomycin D-resistant neurites were formed and these were blocked by K-252a, showing that the inhibitor interferes with the transcription-independent actions of NGF as well. Although the exact mechanism of inhibition of NGF-promoted neurite formation by K-252a is unknown, the most probable explanation is that both transcription-dependent and -independent components are involved in at least one step of the activation of some specific protein kinase(s) that can be suppressed by K-252a.

Roles of ganglioside synthesis on neurite outgrowth of PC12h cells

1988 ◽  
Vol 7 ◽  
pp. S48
Author(s):  
Keisuke Hirai ◽  
Yoshihiro Matsuda ◽  
Hiroshi Hatanaka ◽  
Hachiro Nakagawa
Keyword(s):  
Neurite Outgrowth ◽  
Pc12h Cells

Enhancement of neurite outgrowth in PC12h cells by a protease inhibitor

1988 ◽  
Vol 89 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Yumiko Saito ◽  
Seiichi Kawashima
Keyword(s):  
Protease Inhibitor ◽  
Neurite Outgrowth ◽  
Pc12h Cells
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