scholarly journals A Specific and Sensitive Assay for Gefitinib Using the Enzyme-Linked Immunosorbent Assay in Human Serum

2005 ◽  
Vol 28 (10) ◽  
pp. 1833-1837 ◽  
Author(s):  
Tetsuya Saita ◽  
Hiroshi Fujito ◽  
Masato Mori
Keyword(s):  
Human Serum ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitive Assay

scholarly journals Development and Validation of 13-plex Luminex-Based Assay for Measuring Human Serum Antibodies to Streptococcus pneumoniae Capsular Polysaccharides

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. e00128-18 ◽  
Author(s):  
Danka Pavliakova ◽  
Peter C. Giardina ◽  
Soraya Moghazeh ◽  
Shite Sebastian ◽  
Maya Koster ◽  
...  
Keyword(s):  
Human Serum ◽  
Lower Limit ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Correlate Of Protection ◽  
Immune Correlate ◽  
Relative Standard ◽  
Limit Of Quantitation

ABSTRACT A Luminex-based direct immunoassay (dLIA) platform has been developed to replace the standardized pneumococcal enzyme-linked immunosorbent assay platform. The multiplex dLIA simultaneously measures the concentration of serum immunoglobulin G (IgG) antibodies specific for pneumococcal capsular polysaccharide (PnPS) serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. The assay uses poly-l-lysine (PLL)-conjugated PnPS, chemically coupled to spectrally distinct Luminex microspheres. Assay validation experiments were performed using residual human serum samples obtained from 13-valent pneumococcal conjugate vaccine (13vPnC) clinical studies. Assay results are expressed as IgG antibody concentrations in micrograms per milliliter using the international reference serum, 007sp. The lower limit of quantitation (LLOQ) for all serotypes covered in the 13-plex dLIA fell within the range of 0.002 to 0.038 µg/ml serum IgG. The difference between the lower limit and upper limit of the assay range was >500-fold for all serotypes, and assay variability was <20% relative standard deviation (RSD) for all serotypes. IgG antibody measurements were shown to be serotype-specific (some cross-reactivity was observed only between the structurally related serotypes 6A and 6B as well as 19A and 19F), and no interference was observed between the serotypes when the assay was performed in the 13-plex format compared to the singleplex assays. The 13-plex dLIA platform developed by Pfizer Inc. generates up to 143 test results in a single 96-well plate and is a suitable replacement of the enzyme-linked immunosorbent assay (ELISA) platform for evaluating vaccine clinical trials. IMPORTANCE The pneumococcal enzyme-linked immunosorbent assay (ELISA) measures IgG antibodies in human serum, and it is an important assay that supports licensure of pneumococcal vaccines. The immune correlate of protection, 0.35 µg/ml of IgG antibodies, was determined by the ELISA method. Pfizer has developed a new Luminex-based assay platform to replace the ELISA. These papers describe the important work of (i) validating the Luminex-based assay and (ii) bridging the immune correlate of protection (0.35 µg/ml IgG) to equivalent values reported by the Luminex platform.

Enzyme-linked immunosorbent assay for cholesteryl ester transfer protein in human serum

1995 ◽  
Vol 240 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Toshitaka Sato ◽  
Masayoshi Fukasawa ◽  
Makoto Kinoshita ◽  
Hiroyuki Arai ◽  
Takao Saeki ◽  
...  
Keyword(s):  
Cholesteryl Ester ◽  
Human Serum ◽  
Immunosorbent Assay ◽  
Cholesteryl Ester Transfer Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Transfer Protein

An enzyme-linked immunosorbent assay for fetal steroid binding protein of human serum

Steroids ◽  
1985 ◽  
Vol 45 (1) ◽  
pp. 31-38 ◽  
Author(s):  
M. Jawed Iqbal ◽  
Alastair Forbes ◽  
Timothy P. Corbishley ◽  
Mark L. Wilkinson ◽  
Roger Williams
Keyword(s):  
Human Serum ◽  
Immunosorbent Assay ◽  
Binding Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Steroid Binding ◽  
Steroid Binding Protein

Competitive Direct Enzyme-Linked Immunosorbent Assay for Detection of Sulfamethazine Residues in Swine Urine and Muscle Tissue

1988 ◽  
Vol 71 (6) ◽  
pp. 1137-1140 ◽  
Author(s):  
Deborah E Dixon-Holland ◽  
Stanley E Katz
Keyword(s):  
Horseradish Peroxidase ◽  
Muscle Tissue ◽  
Immunosorbent Assay ◽  
Microtiter Plate ◽  
Test System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitive Assay ◽  
Saline Extract ◽  
Swine Urine ◽  
Phosphate Buffered Saline

Abstract A sensitive assay for the detection of sulfamethazine in swine urine and muscle tissue using a direct competitive enzyme-linked immunosorbent assay (ELISA) has been developed. Undiluted urine or a phosphate-buffered saline extract of pork muscle tissue is mixed with an enzyme-labeled conjugate of sulfamethazine and horseradish peroxidase. The mixture is added to wells of a microtiter plate coated with antibody to sulfamethazine. After the test system is incubated, washed, and re-incubated with substrate and the reaction is stopped, the absorbance is measured at 405 nm. Levels of sulfamethazine as low as 20 ng sulfamethazine/g muscle tissue and 10 ng sulfamethazine/ mL swine urine were detected and estimated

Highly Sensitive, Specific Enzyme-Linked Immunosorbent Assay of Neopterin and Biopterin in Biological Samples

1992 ◽  
Vol 38 (10) ◽  
pp. 1954-1958 ◽  
Author(s):  
S Ogiwara ◽  
K Kiuchi ◽  
T Nagatsu ◽  
R Teradaira ◽  
I Nagatsu ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Present Method ◽  
High Performance ◽  
Microtiter Plate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Usual Manner ◽  
Healthy Control ◽  
Antigen Antibody

Abstract An enzyme immunosorbent assay of neopterin and biopterin on a polystyrene microtiter plate has been developed. A conjugate of neopterin or biopterin to bovine serum albumin was used to raise a specific antiserum against neopterin or biopterin in rabbits. An incubation mixture of the antiserum and samples prepared from human serum underwent another antigen-antibody reaction with the hapten fixed on the microtiter plate. The amount of antibody bound to the fixed hapten, which is inverse to the amount of hapten in the sample, was determined by using anti-rabbit IgG-horseradish peroxidase conjugate in a usual manner by measuring absorbance at 490 nm after reaction with o-phenylenediamine and hydrogen peroxide. The minimal detectable amounts of neopterin and biopterin were approximately 0.1 pmol. The specificity of the assay was so high that the assay system for neopterin completely distinguished it from biopterin, as judged from the cross-reaction of 0.002%, and vice versa. The amounts of neopterin and biopterin in human serum determined by the present method agreed well with those determined by high-performance liquid chromatography. We used the present method to determine the concentrations of neopterin in serum from healthy control subjects and patients with cancers and systemic lupus erythematosus; the results were consistent with literature data.

scholarly journals A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase

2017 ◽  
Vol 58 (8) ◽  
pp. 1591-1597 ◽  
Author(s):  
Kazuya Miyashita ◽  
Katsuyuki Nakajima ◽  
Isamu Fukamachi ◽  
Yuji Muraba ◽  
Takafumi Koga ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunosorbent Assay ◽  
Assay System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatic Triglyceride ◽  
Triglyceride Lipase ◽  
Hepatic Triglyceride Lipase

Development and validation of an enzyme-linked immunosorbent assay for the quantification of trastuzumab in human serum and plasma

2009 ◽  
Vol 391 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Carola W.N. Damen ◽  
Els R. de Groot ◽  
Marianne Heij ◽  
David S. Boss ◽  
Jan H.M. Schellens ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Development And Validation
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