scholarly journals Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

2005 ◽  
Vol 28 (9) ◽  
pp. 1578-1584 ◽  
Author(s):  
Kenji Hamase ◽  
Ryuichi Konno ◽  
Akiko Morikawa ◽  
Kiyoshi Zaitsu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Oxidase Activity ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Sensitive Determination

An Automated Detection System for Most L-α-Amino Acids

1969 ◽  
Vol 15 (2) ◽  
pp. 154-161 ◽  
Author(s):  
K Van Dyke ◽  
C Szustkiewicz
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Detection System ◽  
Automated System ◽  
Acid Oxidase ◽  
Enzymatic Reactions ◽  
Amino Acid Oxidase ◽  
Automated Method ◽  
Wide Range

Abstract An automated system for the determination of the L-α form of the majority of amino acids is presented. The method is based upon oxidative deamination of the amino acid coupled with oxidation of o-dianisidine by hydrogen peroxide. This procedure can be used comparatively for the determination of a mixture of L-α-amino acids or for the majority of separated L-α-amino acids (especially in conjunction with column separations from urine and blood which give falsely positive identification with ninhydrin detection). The stereospecific nature of the L-α-amino acid oxidase enables the investigator to quantitate the amount of L-α-amino acid in the presence of the D-α form. From an academic viewpoint, the extreme sensitivity and wide range of the detection system make it advantageous for the study of the enzyme itself. This automated method also may be employed to follow enzymatic reactions—e.g., those catalyzed by peptidases or racemases. The methodology is extremely convenient with good reagent stability and is much more sensitive than manometric technics.

Determination of Free -Proline and -Leucine in the Brains of Mutant Mice Lacking -Amino Acid Oxidase Activity

2001 ◽  
Vol 298 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Kenji Hamase ◽  
Tomomi Inoue ◽  
Akiko Morikawa ◽  
Ryuichi Konno ◽  
Kiyoshi Zaitsu
Keyword(s):  
Amino Acid ◽  
Oxidase Activity ◽  
Mutant Mice ◽  
Acid Oxidase ◽  
Free Proline ◽  
Amino Acid Oxidase
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