scholarly journals Lipid Membrane-Binding Properties of Tryptophan Analogues of Linear Amphipathic β-Sheet Cationic Antimicrobial Peptides Using Surface Plasmon Resonance

2005 ◽  
Vol 28 (1) ◽  
pp. 148-150 ◽  
Author(s):  
Hiroshi Kamimori ◽  
Jack Blazyk ◽  
Marie-Isabel Aguilar
Keyword(s):  
Surface Plasmon Resonance ◽  
Antimicrobial Peptides ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Lipid Membrane ◽  
Membrane Binding ◽  
Binding Properties ◽  
Cationic Antimicrobial Peptides ◽  
Tryptophan Analogues ◽  
Β Sheet

scholarly journals Binding Properties of Antimicrobial Agents to Lipid Membranes Using Surface Plasmon Resonance

2014 ◽  
Vol 37 (8) ◽  
pp. 1383-1389 ◽  
Author(s):  
Hiroki Kinouchi ◽  
Hirokazu Arimoto ◽  
Kenzo Nishiguchi ◽  
Masako Oka ◽  
Hideki Maki ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Lipid Membranes ◽  
Antimicrobial Agents ◽  
Binding Properties

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

Crotoxin acceptor protein isolated from Torpedo electric organ: binding properties to crotoxin by surface plasmon resonance

Toxicon ◽  
2003 ◽  
Vol 41 (4) ◽  
pp. 509-517 ◽  
Author(s):  
Grazyna Faure ◽  
Alenka Čopič ◽  
Sabine Le Porrier ◽  
Franc Gubenšek ◽  
Cassian Bon ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Electric Organ ◽  
Binding Properties ◽  
Torpedo Electric Organ
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