scholarly journals Inhibitory Effect of Chunghyuldan in Prostaglandin E2 and Nitric Oxide Biosynthesis of Lipopolysaccharide-Induced RAW 264.7 Cells

2004 ◽  
Vol 27 (11) ◽  
pp. 1810-1813 ◽  
Author(s):  
Ki-Ho Cho ◽  
Young-Suk Kim ◽  
Hyung-Sup Bae ◽  
Sang-Kwan Moon ◽  
Woo Sang Jung ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
Raw 264.7

Inhibitory effect of dibutyryl chitin ester on nitric oxide and prostaglandin E2 production in LPS-stimulated RAW 264.7 cells

2012 ◽  
Vol 35 (7) ◽  
pp. 1287-1292 ◽  
Author(s):  
In Hwa Jeon ◽  
Ji Ye Mok ◽  
Kwang-Hyun Park ◽  
Hee Min Hwang ◽  
Mi Seon Song ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
Raw 264.7

Ecklonia stolonifera inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2, and proinflammatory cytokines in RAW264.7 macrophages

Biologia ◽  
2010 ◽  
Vol 65 (2) ◽  
Author(s):  
Eun-Jin Yang ◽  
Young-Min Ham ◽  
Dong Kim ◽  
Ji-Young Kim ◽  
Jung Hong ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Inflammatory Mediators ◽  
Biological Effects ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Ecklonia Stolonifera ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Oxide Synthase

AbstractAs part of our ongoing alternative medicine program, we have directed our attention toward the identification of edible seaweeds in Korea. Here we report on the anti-inflammatory activities of Ecklonia stolonifera. The present study was undertaken to elucidate the pharmacological and biological effects of E. stolonifera extracts on the production of inflammatory mediators in macrophages. The results indicate that the hexane fraction of E. stolonifera extract (ESH) is an effective inhibitor of lipopolysccharide (LPS)-induced NO, prostaglandin E2, and proinflammatory cytokine production in RAW 264.7 cells. These inhibitory effects of ESH were accompanied by decreases in the expression of inducible nitric oxide synthase and cyclooxygenase-2 proteins. Furthermore, ESH inhibited the LPS-induced phosphorylation and degradation of IκB-α, which is required for the nuclear translocations of the p50 and p65 nuclear transcription factor kappa-B (NF-κB) subunits in RAW 264.7 cells. Our results suggest that ESH might exert an anti-inflammatory effect by inhibiting the expression of pro-inflammatory cytokines. Such an effect is mediated by a blocking of NF-κB activation, which consequently inhibits the generation of inflammatory mediators in RAW264.7 cells. Through HPLC fingerprinting of the E. stolonifera extract, the phloroglucinol was also identified and quantified as standard substance. Moreover, we tested the potential application of E. stolonifera extract as a cosmetic material by performing human skin primary irritation tests. In these assays, E. stolonifera extracts did not induce any adverse reactions. Based on these results, we suggest that E. stolonifera extracts be considered possible anti-inflammatory candidates for topical application.

Fermentation Improves Anti-Inflammatory Effect of Sipjeondaebotang on LPS-Stimulated RAW 264.7 Cells

2012 ◽  
Vol 40 (04) ◽  
pp. 813-831 ◽  
Author(s):  
You-Chang Oh ◽  
Won-Kyung Cho ◽  
Yun Hee Jeong ◽  
Ga Young Im ◽  
Min Cheol Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Mediators ◽  
High Performance ◽  
Biological Effects ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Tnf Α ◽  
Cox 2 ◽  
Anti Inflammatory

Sipjeondaebotang (SJ) has been used as a traditional drug in east-Asian countries. In this study, to provide insight into the biological effects of SJ and SJ fermented by Lactobacillus, we investigated their effects on lipopolysaccharide (LPS)-mediated inflammation in macrophages. The investigation was focused on whether SJ and fermented SJ could inhibit the production of pro-inflammatory mediators such as prostaglandin (PG) E2 and nitric oxide (NO) as well as the expressions of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB in LPS-stimulated RAW 264.7 cells. We found that SJ modestly inhibited LPS-induced PGE2, NO and TNF-α production as well as the expressions of COX-2 and iNOS. Interestingly, fermentation significantly increased its inhibitory effect on the expression of all pro-inflammatory mediators. Furthermore, fermented SJ exhibited increased inhibition of p38 MAPK and c-Jun NH2-terminal kinase (JNK) MAPK phosphorylation as well as NF-κB p65 translocation by reduced IκBα degradation compared with either untreated controls or unfermented SJ. High performance liquid chromatography (HPLC) analysis showed fermentation by Lactobacillus increases liquiritigenin and cinnamyl alcohol contained in SJ, which are known for their anti-inflammatory activities. Finally, SJ fermented by Lactobacillus exerted potent anti-inflammatory activity by inhibiting MAPK and NF-κB signaling in RAW 264.7 cells.

Inhibitory effects of Irigenin from the rhizomes of Belamcanda chinensis on nitric oxide and prostaglandin E2 production in murine macrophage RAW 264.7 cells

Life Sciences ◽  
2006 ◽  
Vol 78 (20) ◽  
pp. 2336-2342 ◽  
Author(s):  
Kwang Seok Ahn ◽  
Eun Jung Noh ◽  
Kwang-Hyun Cha ◽  
Yeong Shik Kim ◽  
Soon Sung Lim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Inhibitory Effects ◽  
Belamcanda Chinensis

Inhibitory effect of DCDC on lipopolysaccharide-induced nitric oxide synthesis in RAW 264.7 cells

Life Sciences ◽  
2001 ◽  
Vol 68 (21) ◽  
pp. 2435-2447 ◽  
Author(s):  
Yu-Chun Huang ◽  
Jih-Hwa Guh ◽  
Zhi-Jiao Cheng ◽  
Ya-Ling Chang ◽  
Tsong-Long Hwang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Effect ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Nitric Oxide Synthesis

Lansoprazole Inhibits Nitric Oxide and Prostaglandin E2 Production in Murine Macrophage RAW 264.7 Cells

Inflammation ◽  
2011 ◽  
Vol 35 (3) ◽  
pp. 1062-1068 ◽  
Author(s):  
Shuji Nakagawa ◽  
Yuji Arai ◽  
Tsunao Kishida ◽  
Nobuyuki Hiraoka ◽  
Shinji Tsuchida ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage
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