scholarly journals Evaluation of the Nitric Oxide Radical Scavenging Activity of Manganese Complexes of Curcumin and Its Derivative

2004 ◽  
Vol 27 (2) ◽  
pp. 170-173 ◽  
Author(s):  
Yaowared Sumanont ◽  
Yukihisa Murakami ◽  
Michihisa Tohda ◽  
Opa Vajragupta ◽  
Kinzo Matsumoto ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Manganese Complexes ◽  
Scavenging Activity ◽  
Nitric Oxide Radical

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF ROOT EXTRACTS OF GLYCYRRHIZA GLABRA L

2016 ◽  
Vol 9 (6) ◽  
pp. 85
Author(s):  
Archana Sakthi
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Glycyrrhiza Glabra ◽  
Peroxide Radical ◽  
Scavenging Activity ◽  
Root Extracts ◽  
Nitric Oxide Radical ◽  
Glycyrrhiza Glabra L

ABSTRACT:     Obesity is an international health problem caused by various environmental and genetic factors. Herbal supplements have proved to be active against obesity. Glycyrrhiza glabra belongs to the family of Fabaceae. Glycyrrhiza glabra was used traditionally to treat various diseases. Hence the current study was planned to investigate the phytochemicals and antioxidants present in Glycyrrhiza glabra. Materials and Methods: The preliminary qualitative, quantitative phytochemicals and antioxidants were investigated from the root extracts of Glycyrrhiza glabra. Results: The preliminary qualitative analysis of various extracts of Glycyrrhiza glabra showed the presence of Phenols, Flavonoids, Tannins, Saponins and Quinones. Quantitative analysis of Glycyrrhiza glabra showed elevated amounts of phenols, flavonoids and tannins in ethanolic extracts. The Aqueous, ethanol, hydro alcoholic extracts of Glycyrrhiza glabra showed 211±2.64, 281.66±1.57, 263±14.2 mg of phenols, 152.66±3.05, 187.33±1.52, 183.33±3.05 mg of flavonoids, 21.33±0.57, 41±3.46, 34.3±1.15 mg of Tannins, 42.33±2.05, 63.33±1.52, 36.33±1.57 mg of terpenoids. The antioxidant properties of the ethanolic extract of Glycyrrhiza glabra were evaluated with the 2,2-diphenyl-1-picryl hydrazyl (DPPH), Nitric Oxide radical (NO), Superoxide radical (SO), Hydrogen peroxide radical (H2O2), Hydroxyl radical (HO) Scavenging activity. The IC50 value of Glycyrrhiza glabra is 120 µg/ml for DPPH, 208 µg/ml for (NO), 196 µg/ml for (SO), 148 µg/ml for (H2O2), and 252 µg/ml for (OH). Invitro method suggests that Glycyrrhiza glabra is more powerful against nitric oxide and hydroxyl radicals. Conclusion: This preliminary study shows that Glycyrrhiza glabra is a promising herb with many phytochemicals and antioxidants it can be further investigated for its antiobesity potential.Key words: Glycyrrhiza glabra L., phytochemicals, antioxidant and obesity.

scholarly journals Effects of Some Heavy Metals (Cd, Cu, Pb, and Zn) Concentration on Qualitative Traits of Dill (Anethum graveolens L.) and Basil (Ocimum basilicum L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 54-67
Author(s):  
Amir RAHİMİ ◽  
Gülen ÖZYAZICI ◽  
Fatemeh AHMADİ
Keyword(s):  
Nitric Oxide ◽  
Heavy Metals ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ocimum Basilicum ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Anethum Graveolens ◽  
Zn Concentration ◽  
Nitric Oxide Radical

Soil pollution is a global concern from past to recent. Toxic and high resistance nature of chemical pollutants, such as heavy metals show their important as environmental risk and a serious problem for human health, because they can introduce to the food chain from contaminated environments. Experiments were conducted to evaluate the effect of Cd, Cu, Pb, and Zn on qualitative parameters of dill (Anethum graveolens L.), and basil (Ocimum basilicum L.) grown in polluted soil environment. Heavy metals concentration, total phenol and flavonoid, essential oil percentage, DPPH radical scavenging activity, super and nitric oxide radical scavenging activity in leave and stem parts of the plants were analysed in this study according to standard methods. According to results, Cu was the dominant heavy metal in leave and stem of both studied plants, followed by Pb, Zn, and Cd. The concentration of various heavy metals in dill was more than two times higher than basil. Total phenol and flavonoids showed significant differences in leave and stem in both plants. DPPH, super oxide and nitric oxide radical scavenging activity showed significant higher values in leave than stem in both plants.

scholarly journals STUDY OF ANTIOXIDANT ACTIVITY OF FORMONONETIN BY IN VITRO METHOD

2017 ◽  
Vol 9 (2) ◽  
pp. 273
Author(s):  
V. J. Vishnuvathan ◽  
K. S. Lakshmi ◽  
A. R. Srividya
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radicals ◽  
Inhibitory Activity ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Nitric Oxide Radical

<p><strong>Objective: </strong>Now the important field of research in phytomedicine is to search a new plant source as well as new phytoconstituents that have fewer side effects and low cost with the free radical scavenging activity<strong>. </strong></p><p><strong>Methods</strong>:<strong> </strong>Different <em>in vitro</em> models such as DPPH, hydroxyl radical, hydrogen peroxide radical, nitric oxide radical and superoxide radical scavenging activity were performed using different concentrations of formononetin ranging from 0.1-50 μg/ml.</p><p><strong>Results: </strong>Formononetin showed only 84.39% inhibitory activity against DPPH radical and it was found to be 16 % less than the butylated hydroxytoluene with the IC <sub>50 </sub>value of 4.65 μg/ml concentration. In scavenging hydroxyl radicals, formononetin inhibited only 67.63% with the IC<sub>50</sub> value of 9.48 μg/ml concentration and it showed 12 % lesser inhibitory activity than standard alpha-tocopherol. Formononetin could able to scavenge maximum 73.53% of hydrogen peroxide radicals with the IC<sub>50 </sub>value of 4.75 μg/ml and its activity of scavenging hydrogen peroxide were found to be 10 % less than the standard ascorbic acid. Formononetin showed 94.33 % nitric oxide radical inhibitory activity with the IC<sub>50 </sub>value of 5.0 μg/ml and it showed 6% lesser activity when compared to standard quercetin. Formononetin showed 94.79 % activity against the scavenging of superoxide radical with the IC<sub>50</sub> value of 4.27 μg/ml. Superoxide inhibitory activity of formononetin was 5.3 %less than the standard quercetin.</p><p><strong>Conclusion: </strong>All these results suggested that, formononetin is a good natural antioxidant which is capable of scavenging almost all types of free radicals. So this could be used to treat various diseases like diabetes, atherosclerosis, cancers, the aging and cardiovascular disease which is being caused by free radicals.</p>

IN VITRO ANTIOXIDANT AND ALPHA-AMYLASE, ALPHA-GLUCOSIDASE INHIBITORY ACTIVITY OF FLOWERS OF CALOTROPIS GIGANTEA

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (10) ◽  
pp. 38-42
Author(s):  
N. K Choudhary ◽  
◽  
J Dwivedi ◽  
S Sharma
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Antidiabetic Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The present investigations were carried out to evaluate the in vitro antioxidant as well as antidiabetic activity of flowers of Calotropis gigantea. Different extracts (petroleum ether, chloroform and ethanolic extract) were prepared using successive solvent extraction method (soxhlet) and screened for its in vitro antioxidant activity using Diphenyl picryl hydrazyl (DPPH) radical scavenging activity, ABT S radical cation decolorization assay and nitric oxide (NO) radical scavenging activity and IC50 were calculated. In vitro antidiabetic activity was studied using α – amylase and α – glucosidase inhibitory assay. Chloroform extract, among the three extracts (i.e. petroleum ether, chloroform and ethanolic), showed maximum antioxidant activity with IC50 value of 151.23µg/ml, 73.56 µg/ml and 158.92µg/ml against DPPH radical scavenging activity, ABTS radical cation decolorization assay and nitric oxide (NO) radical scavenging activity respectively. The chloroform extract produced a significant in vitro antidiabetic activity with IC50 of 52.3µg/ml 18.2µg/ml against α – amylase and α – glucosidase enzymes but less inhibitory effect than standard acarbose.

scholarly journals Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

2015 ◽  
Vol 7 ◽  
Author(s):  
Geena Mariya Jose ◽  
Anitha Radhakrishnan ◽  
G Muraleedhara Kurup
Keyword(s):  
Antioxidant Activity ◽  
Brown Algae ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sulfated Polysaccharide ◽  
Sulfated Polysaccharides ◽  
Scavenging Activity ◽  
Padina Tetrastromatica ◽  
Nitric Oxide Radical

<p class="Default">Antioxidants play a central role in the prevention of carcinogenesis. The most natural compounds exhibit their protective effects by eliciting antioxidant potential. Sulfated polysaccharide was isolated from the brown algae <em>Padina tetrastromatica</em>, then purified and evaluated for its composition and <em>in vitro </em>antioxidant and antimitotic activities. Both ethanolic sulfated polysaccharide (ESPS) and ethanolic sulfated polysaccharide-column purified (ESPS-CP) exhibited considerable amount of carbohydrates (11.2% and 17.6%), sulfate (11.4% and 7.4%), fucose (5.5% and 15.7%), uronic acid (4.7% and 11.8%), xylose (0.5% and 0.03%) and sulfated polysaccharide (2.4% and 12.7%) content. The FTIR analysis and phytochemical screening also confirmed the presence of sulfated polysaccharides. In the <em>in vitro</em> antioxidant activity determination using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity and reducing power, ESPS showed more activity than ESPS-CP. In the case of nitric oxide radical scavenging, ESPS-CP was found to be more effective. At a concentration of 2mg/ml, both samples were potent antioxidants with significant IC<sub>50</sub> values. The antimitotic studies such as mitotic index in onion root tips and sprouting assay in green gram seeds also proved that both the extracts are able to prevent mitosis. The extrapolation of these results can find opportunities in therapeutic regiments of cancer.<strong></strong></p>

scholarly journals Free Radical Scavenging Activity of Ethanolic Extract of Citrus paradisi and Naringin -An In vitro Study

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
R Roghini ◽  
K. Vijayalakshmi
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Citrus Paradisi

The present study attempts to find naturally-occurring antioxidants of fruit-based which give efficacy by additive activities. Citrus paradisi, known as Grape fruit contains significant bioactive components such as Naringin. The present study examines the free radical scavenging activity of ethanolic extract of Citrus paradisi and Naringin. The study was carried out with different radical scavenging assays like hydroxyl, DPPH, hydrogen peroxide, nitric oxide, super oxide. Citrus paradisi extracts showed lower radical scavenging activities in assays such as DPPH, superoxide and hydroxyl when compared with Naringin. Naringin showed the higher radical scavenging effect with nitric oxide, and hydrogen peroxide in comparison with citrus paradisi extract. However, both were analysed by using ascorbic acid as standard. The current study gives evidence that both showed potential free radical scavenging activity.

scholarly journals EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF MIMOSA PUDICA THORNS

2018 ◽  
Vol 11 (11) ◽  
pp. 153 ◽  
Author(s):  
Lakshmibai R ◽  
Amirtham D
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Mimosa Pudica ◽  
Age Related ◽  
Dpph Method

Objective: Mimosa pudica is a traditional medicinal plant. The natural antioxidant compounds from plants mop up the free radicals causing cell damage and maintain the biological systems. The aim of the present study is to evaluate the free radical scavenging potential of the ethanolic and aqueous thorn extracts of M. pudica.Methods: The ethanolic and aqueous thorn extracts of M. pudica were analyzed for the phytochemicals and for free radical scavenging activity by 1, 1 diphenyl 1-2-picric hydrazine (DPPH), 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and nitric oxide free radical scavenging method.Results: Flavonoids, saponins, glycosides, alkaloids, terpenoids, and coumarins were the phytoconstituents revealed in ethanolic and aqueous thorn extracts of M. pudica. At 250 μg/mL concentrations, aqueous thorn extracts of M. pudica exhibited 73.41% radical scavenging activity by DPPH method and 26.10% inhibition by nitric oxide free radical scavenging method. However, the ethanolic extracts of M. pudica thorns exhibited 73.35% inhibition by ABTS free radical scavenging method at 250 μg/mL concentrations.Conclusion: The results obtained suggest that the plant extracts from M. pudica could serve as a potential source of antioxidant in slowing down the process of aging and age-related or oxidative stress-related degenerative diseases. Moreover, the isolation of bioactive principle responsible for the antioxidant activity and formulation of novel therapeutic agents can be further studied.

Radical Scavenging Activities of Heracleum aquilegifolium Wight (Apiaceae) Fruit Oils in vitro

2010 ◽  
Vol 65 (11-12) ◽  
pp. 653-659 ◽  
Author(s):  
Subbiah Karuppusamy ◽  
Gurunathan Muthuraja
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Nitric Oxide Radical

The fruits of Heracleum aquilegifolium Wight (Apiaceae) were collected from Western Ghats of the Indian Peninsula. The essential oils were extracted by hydrodistillation. The chemical composition of the essential oils was analysed by gas chromatography and gas chromatography-mass spectrometry (GC-MS). β -Pinene (22.3%), 1,8-cineole (20.3%), and β-phellandrene (12.4%) were the main components of H. aquilegifolium fruit oils. The antioxidant properties of essential oils of H. aquilegifolium were examined by different procedures namely reducing power ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide radical scavenging activity, hydrogen peroxide scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, and metal chelating activity. The antioxidant activities were compared with those of synthetic antioxidants and standard drugs such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ascorbic acid, α-tocopherol, curcumin, and quercetin. The study confirmed the possible antioxidant potential of essential oils tested with various in vitro antioxidant methods. The presence of monoterpenes in combination with other components in the oils could be responsible for the activity

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