scholarly journals Oxidosqualene Cyclases from Cell Suspension Cultures of Betula platyphylla var. japonica: Molecular Evolution of Oxidosqualene Cyclases in Higher Plants

2003 ◽  
Vol 26 (5) ◽  
pp. 642-650 ◽  
Author(s):  
Hong Zhang ◽  
Masaaki Shibuya ◽  
Shinso Yokota ◽  
Yutaka Ebizuka
1972 ◽  
Vol 50 (10) ◽  
pp. 2031-2037 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll

High yields of extracellular polysaccharide were obtained from cell suspension cultures of root, hypocotyl, and cotyledon of bush bean. Hydrolysates of the three polysaccharide samples contained the same sugars: galacturonic acid, galactose, glucose, mannose, arabinose, and xylose. The relative amounts of the six sugars were not the same in the hydrolysates from the three sources. The extracellular polysaccharide was produced at all times during the culture cycle. Semilogarithmic plots of increase in cell number, and production of extracellular polysaccharide, indicate that production per cell decreased during the logarithmic phase, and increased at the onset of the stationary phase. Production of extracellular polysaccharide, per culture and per cell, was much higher than that reported for other cell cultures of higher plants.


2011 ◽  
Vol 335-336 ◽  
pp. 105-110 ◽  
Author(s):  
Qiao Li Zhai ◽  
Ya Guang Zhan ◽  
Dao Qi Xu ◽  
Xiao Dong Wang ◽  
Gui Zhi Fan

58 fungal elicitors prepared from the endophytic fungi isolated from inner bark of B. platyphylla Suk. were examined to determine their effects on the growth and triterpenoid production in suspension cultures of Betula platyphylla Suk. cells. The results showed that different fungal elicitors could cause diversely stimulating effects. Among the fungal elicitors tested, BE58 stimulated the highest triterpenoids yield with 15.90 mg•L-1 and 183.72% higher than the control. The experiment also affirmed from mRNA level that triterpenoid was indeed accumulated in suspension culture of birch cells treated by BE58 fungal elicitor. Meantime BE58 was identified as Phomopsis from the morphological and molecular level.


1996 ◽  
Vol 13 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Yohichi WAKITA ◽  
Hamako SASAMOTO ◽  
Shinso YOKOTA ◽  
Nobuo YOSHIZAWA

1976 ◽  
Vol 54 (24) ◽  
pp. 2924-2927 ◽  
Author(s):  
Donald K. Dougall ◽  
Jeff Bloch

Evidence was sought for the presence of glutamate synthase (EC 2.6.1.53) in extracts from suspension cultures of six higher plant species not previously examined. The level of glutamate synthase measured was above the level of glutamic dehydrogenase (EC 1.4.1.2, 1.4.1.4) in extracts of soybean, parsley, okra, and cotton. Glutamate synthase was detectable but less than glutamic dehydrogenase in extracts of sugarcane. Glutamate synthase was not detected in extracts of peanut. Evidence for two glutamate synthases, each specific for one pyridine nucleotide, was obtained with cultures of carrot. Glutamate synthase has now been detected in eight and possibly nine species representing four and possibly five families of higher plants.


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