scholarly journals DNA-Binding Activity and Cytotoxicity of the Extended Diphenylfuran Bisamidines in Breast Cancer MCF-7 Cells.

2001 ◽  
Vol 24 (6) ◽  
pp. 704-706 ◽  
Author(s):  
Krzysztof BIELAWSKI ◽  
Slawomir WOLCZYNSKI ◽  
Anna BIELAWSKA
Keyword(s):  
Breast Cancer ◽  
Dna Binding ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Mcf 7

scholarly journals Deleted in Breast Cancer 1, a Novel Androgen Receptor (AR) Coactivator That Promotes AR DNA-binding Activity

2009 ◽  
Vol 284 (11) ◽  
pp. 6832-6840 ◽  
Author(s):  
Junjiang Fu ◽  
Jun Jiang ◽  
Jiwen Li ◽  
Shanshan Wang ◽  
Guang Shi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Androgen Receptor ◽  
Dna Binding ◽  
Binding Activity ◽  
Dna Binding Activity

scholarly journals Inhibition of estrogen-responsive gene activation by the retinoid X receptor beta: evidence for multiple inhibitory pathways.

1993 ◽  
Vol 13 (4) ◽  
pp. 2258-2268 ◽  
Author(s):  
J H Segars ◽  
M S Marks ◽  
S Hirschfeld ◽  
P H Driggers ◽  
E Martinez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hormone Receptors ◽  
Gene Activation ◽  
Cancer Cell Line ◽  
Binding Activity ◽  
Retinoid X Receptor ◽  
Reporter Activity ◽  
Dna Binding Activity ◽  
Receptor Beta ◽  
Mcf 7

The retinoid X receptor beta (RXR beta; H-2RIIBP) forms heterodimers with various nuclear hormone receptors and binds multiple hormone response elements, including the estrogen response element (ERE). In this report, we show that endogenous RXR beta contributes to ERE binding activity in nuclear extracts of the human breast cancer cell line MCF-7. To define a possible regulatory role of RXR beta regarding estrogen-responsive transcription in breast cancer cells, RXR beta and a reporter gene driven by the vitellogenin A2 ERE were transfected into estrogen-treated MCF-7 cells. RXR beta inhibited ERE-driven reporter activity in a dose-dependent and element-specific fashion. This inhibition occurred in the absence of the RXR ligand 9-cis retinoic acid. The RXR beta-induced inhibition was specific for estrogen receptor (ER)-mediated ERE activation because inhibition was observed in ER-negative MDA-MB-231 cells only following transfection of the estrogen-activated ER. No inhibition of the basal reporter activity was observed. The inhibition was not caused by simple competition of RXR beta with the ER for ERE binding, since deletion mutants retaining DNA binding activity but lacking the N-terminal or C-terminal domain failed to inhibit reporter activity. In addition, cross-linking studies indicated the presence of an auxiliary nuclear factor present in MCF-7 cells that contributed to RXR beta binding of the ERE. Studies using known heterodimerization partners of RXR beta confirmed that RXR beta/triiodothyronine receptor alpha heterodimers avidly bind the ERE but revealed the existence of another triiodothyronine-independent pathway of ERE inhibition. These results indicate that estrogen-responsive genes may be negatively regulated by RXR beta through two distinct pathways.

The licorice flavonoid isoliquiritigenin reduces DNA-binding activity of AhR in MCF-7 cells

2014 ◽  
Vol 221 ◽  
pp. 70-76 ◽  
Author(s):  
Tsz Yan Wong ◽  
Shu-mei Lin ◽  
Ching Ho Poon ◽  
Lai K. Leung
Keyword(s):  
Dna Binding ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Mcf 7

Bovine dialyzable leukocyte extract modulates AP-1 DNA-binding activity and nuclear transcription factor expression in MCF-7 breast cancer cells

Cytotherapy ◽  
2008 ◽  
Vol 10 (2) ◽  
pp. 212-219 ◽  
Author(s):  
E. Mendoza-Gamboa ◽  
M.A. Franco-Molina ◽  
P. Zapata-Benavides ◽  
P. Castillo-Tello ◽  
M.E. Vera-García ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Binding Activity ◽  
Nuclear Transcription Factor ◽  
Dna Binding Activity ◽  
Factor Expression ◽  
Transcription Factor Expression ◽  
Mcf 7
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