scholarly journals A Novel Van91 I Polymorphism in the 1st Intron of the Parathyroid Hormone(PTH)/PTH-Related Peptide(PTHrP) Receptor Gene and Its Effect on the Urinary cAMP Response to PTH.

2000 ◽  
Vol 23 (4) ◽  
pp. 386-389 ◽  
Author(s):  
Masayuki HEISHI ◽  
Hiromitsu TAZAWA ◽  
Takatoshi MATSUO ◽  
Takayuki SARUTA ◽  
Masato HANAOKA ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Receptor Gene ◽  
Related Peptide ◽  
Pthrp Receptor

scholarly journals The transcription factors SP1 and MAZ regulate expression of the parathyroid hormone/parathyroid hormone-related peptide receptor gene

2000 ◽  
Vol 25 (3) ◽  
pp. 309-319 ◽  
Author(s):  
LJ Williams ◽  
AB Abou-Samra
Keyword(s):  
Transcription Factors ◽  
Parathyroid Hormone ◽  
Mutational Analysis ◽  
Receptor Gene ◽  
Dna Interaction ◽  
Osteosarcoma Cell ◽  
Specific Expression ◽  
Related Peptide ◽  
Its Gene ◽  
Pthrp Receptor

The parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor regulates extracellular calcium concentrations and is therefore important for mineral homeostasis. ROS 17/2.8 cells, a rat osteoblast-like osteosarcoma cell line, express the PTH/PTHrP receptor and provide a good model for examining the transcriptional regulation of its gene. The rat PTH/PTHrP receptor gene has two promoters, U1 and U3, which were shown to be important for its expression. Using extracts from ROS 17/2.8 cells, we have demonstrated two regions (termed FP1 and FP2) of nuclear protein/DNA interaction within promoter sequences previously shown to be important for the activity of the U3 promoter. Nuclear extracts from rat 2 fibroblasts, which do not express the PTH/PTHrP receptor, produced one site of protein/DNA interaction which was found at a position on the promoter identical to the position of FP1 produced by a ROS 17/2.8 nuclear extract. Mutation of these two sites of protein/DNA interaction resulted in reduced U3 promoter activity. Furthermore, we have demonstrated that the transcription factors SP1 and MAZ regulate U3 promoter expression and have shown their functional significance using mutational analysis. These data demonstrate that SP1 and MAZ bind to the PTH/PTHrP receptor promoter and that they are involved in cell-specific expression of its gene product.

Expression of parathyroid hormone-related peptide (PTHrP) and PTH/PTHrP receptor in newborn human calvaria osteoblastic cells

1997 ◽  
Vol 136 (6) ◽  
pp. 640-648 ◽  
Author(s):  
Abderrahim Lomri ◽  
Cindy de Pollak ◽  
Michael Sebag ◽  
David Goltzman ◽  
Richard Kremer ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Culture Media ◽  
Receptor Expression ◽  
Osteoblastic Cells ◽  
Pcr Analysis ◽  
Intracellular Camp ◽  
Related Peptide ◽  
Parathyroid Hormone Related Peptide ◽  
Pthrp Receptor

Abstract We examined the expression of parathyroid hormone-related peptide (PTHrP) and its receptor in normal newborn human calvaria osteoblastic (NHCO) cells. Northern blot analysis showed that NHCO cells express a single 1·6 kb transcript of PTHrP, which was increased within 1 h (2x) and peaked at 6 h (7x) after serum treatment. In the culture media, the release of PTHrP peptide was maximally increased (4x) 24 h after the addition of serum, as determined by immunoradiometric assay. NHCO cells exhibited a cytoplasmic immunostaining for PTHrP in the presence of serum, and most PTHrP-positive cells were alkaline phosphatase-negative, suggesting that PTHrP was expressed in undifferentiated cells. Furthermore, RT-PCR analysis showed that both PTHrP and PTH/PTHrP receptor were expressed in NHCO cells in basal conditions or after stimulation with serum. The maximal PTHrP expression induced by serum suppressed PTH/PTHrP receptor expression, suggesting that PTHrP down-regulated its receptor in NHCO cells. Treatment with 10 nm human PTH(1–34—which binds to PTH/PTHrP receptors, increased intracellular cAMP levels and alkaline phosphatase activity, and decreased cell growth, indicating that ligand binding to PTH/PTHrP receptors regulates NHCO cell proliferation and differentiation. The expression and synthesis of PTHrP and the presence of functional PTH/PTHrP receptors suggest a possible paracrine mechanism of action of PTHrP in normal human calvaria osteoblastic cells. European Journal of Endocrinology 136 640–648

scholarly journals Calcium fluxes at the bone/plasma interface: Acute effects of parathyroid hormone (PTH) and targeted deletion of PTH/PTH-related peptide (PTHrP) receptor in the osteocytes

Bone ◽  
2018 ◽  
Vol 116 ◽  
pp. 135-143 ◽  
Author(s):  
Christopher Dedic ◽  
Tin Shing Hung ◽  
Alan M. Shipley ◽  
Akira Maeda ◽  
Thomas Gardella ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Acute Effects ◽  
Calcium Fluxes ◽  
Targeted Deletion ◽  
Related Peptide ◽  
Pthrp Receptor

Expression of Parathyroid Hormone (PTH)-related Peptide (PTHrP) and PTH/PTHrP Receptor in Osteoclast-like Giant Cells

2003 ◽  
Vol 199 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Masahiro Nakashima ◽  
Toshiyuki Nakayama ◽  
Akira Ohtsuru ◽  
Eiichiro Fukada ◽  
Daisuke Niino ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Giant Cells ◽  
Related Peptide ◽  
Pthrp Receptor
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