scholarly journals Adhesive Interaction of Highly Malignant Hepatoma AH66F Cells with Mesothelial Cells.

1999 ◽  
Vol 22 (7) ◽  
pp. 738-740 ◽  
Author(s):  
Masaaki NOMURA ◽  
Yuhki SUGIURA ◽  
Yasuaki TATSUMI ◽  
Ken-ichi MIYAMOTO
Keyword(s):  
Mesothelial Cells ◽  
Adhesive Interaction ◽  
Malignant Hepatoma

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

A method for determining the natural moisture content of dentin in a clinical setting

2020 ◽  
Vol 1 (12) ◽  
pp. 36-39
Author(s):  
L. V. Iyashvili ◽  
Yu. A. Vinnichenko ◽  
A. V. Vinnichenko
Keyword(s):  
Composite Material ◽  
Moisture Content ◽  
Computer Program ◽  
Clinical Setting ◽  
Quantitative Assessment ◽  
Critical Mass ◽  
Filling Material ◽  
Adhesive Interaction ◽  
Natural Moisture Content ◽  
Hard Tissues

The purpose of the study is a quantitative assessment of the yield of dentinal fluid on the surface of the treated dentin of the tooth when restoring its structure with a composite filling material. To achieve this goal, digital images of the coronal parts of the teeth having formed carious cavities were used; virtual models of hard tissues of teeth recreated using specialized computer programs; A computer program that provides the ability to accurately determine the area of the treated dentin tooth. The results made it possible to draw the following conclusions: with an increase in the depth of the carious cavity, the amount of dentin fluid that can stand out on its surface (1–2 mm from the tooth cavity) sharply increases; with an increase in the area of the formed carious cavity (more than 30 mm2), the risk of release of a critical mass of dentinal fluid (more than 0.4 mg), which can adversely affect the strength of the adhesive interaction between the composite material and the hard tissues of the tooth, increases significantly; the same dynamics is observed with increasing time, at which there is the possibility of free exit of dentinal fluid to the surface of the cavity prepared for filling (more than 45 seconds).

Effects of Saline and Other Peritoneal Lavage Solutions on the Morphology and Function of in Vitro Mesothelial Cells

2007 ◽  
Vol 79 (8) ◽  
Author(s):  
Marek Winckiewicz ◽  
Alicja Połubińska ◽  
Ryszard Staniszewski ◽  
Andrzej Bręborowicz
Keyword(s):  
Peritoneal Lavage ◽  
Mesothelial Cells ◽  
And Function

Time Series Analysis of Transmesothelial Invasion by Endometrial Stromal and Epithelial Cells Using Three-dimensional Confocal Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 580-581
Author(s):  
CA Witz ◽  
S Cho ◽  
VE Centonze ◽  
IA Montoya-Rodriguez ◽  
RS Schenken
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Time Course ◽  
Three Dimensional ◽  
Collagen Iv ◽  
Mesothelial Cells ◽  
Endometrial Stromal Cells ◽  
Human Collagen ◽  
Endometrial Epithelial Cells

Using human peritoneal explants, we have previously demonstrated that endometrial stromal cells (ESCs) and endometrial epithelial cells (EECs) attach to intact mesothelium. Attachment occurs within one hour and mesothelial invasion occurs within 18 hours (Figure 1). We have also demonstrated that, in vivo, the mesothelium overlies a continuous layer of collagen IV (Col IV).More recently we have used CLSM, to study the mechanism and time course of ESC and EEC attachment and invasion through mesothelial monolayers. in these studies, CellTracker® dyes were used to label cells. Mesothelial cells were labeled with chloromethylbenzoylaminotetramethylrhodamine (CellTracker Orange). Mesothelial cells were then plated on human collagen IV coated, laser etched coverslips. Mesothelial cells were cultured to subconfluence. ESCs and EECs, labeled with chloromethylfluorscein diacetate (CellTracker Green) were plated on the mesothelial monolayers. Cultures were examined at 1, 6, 12 and 24 hours with simultaneous differential interference contrast and CLSM.

Lrp1 Regulates Urokinase Receptor-Dependent Pathophysiologic Responses Of Human Pleural Mesothelial Cells

2011 ◽  
Author(s):  
Torry A. Tucker ◽  
LaTerrica Williams ◽  
Kathy Koenig ◽  
Hema Kothari ◽  
Andrey Komissarov ◽  
...  
Keyword(s):  
Mesothelial Cells ◽  
Urokinase Receptor ◽  
Pleural Mesothelial Cells

scholarly journals Investigation of the Wear Behavior of PVD Coated Carbide Tools during Ti6Al4V Machining with Intensive Built Up Edge Formation

Coatings ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 266
Author(s):  
M.S.I. Chowdhury ◽  
B. Bose ◽  
S. Rawal ◽  
G.S. Fox-Rabinovich ◽  
S.C. Veldhuis
Keyword(s):  
Tool Wear ◽  
Titanium Alloys ◽  
Wear Behavior ◽  
Cutting Temperature ◽  
Ti6al4v Alloy ◽  
Adhesive Interaction ◽  
Heavy Load ◽  
Micromechanical Properties ◽  
Rough Turning ◽  
Coated Carbide

Tool wear phenomena during the machining of titanium alloys are very complex. Severe adhesive interaction at the tool chip interface, especially at low cutting speeds, leads to intensive Built Up Edge (BUE) formation. Additionally, a high cutting temperature causes rapid wear in the carbide inserts due to the low thermal conductivity of titanium alloys. The current research studies the effect of AlTiN and CrN PVD coatings deposited on cutting tools during the rough turning of a Ti6Al4V alloy with severe BUE formation. Tool wear characteristics were evaluated in detail using a Scanning Electron Microscope (SEM) and volumetric wear measurements. Chip morphology analysis was conducted to assess the in situ tribological performance of the coatings. A high temperature–heavy load tribometer that mimics machining conditions was used to analyze the frictional behavior of the coatings. The micromechanical properties of the coatings were also investigated to gain a better understanding of the coating performance. It was demonstrated that the CrN coating possess unique micromechanical properties and tribological adaptive characteristics that minimize BUE formation and significantly improve tool performance during the machining of the Ti6Al4V alloy.

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