scholarly journals Inhibitory Effect of Polyoxotungstates on the Production of Penicillin-binding Proteins and .BETA.-Lactamase against Methicillin-resistant Staphylococcus aureus.

1999 ◽  
Vol 22 (5) ◽  
pp. 463-470 ◽  
Author(s):  
Norio FUKUDA ◽  
Toshihiro YAMASE ◽  
Yutaka TAJIMA
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Proteins ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Effect ◽  
Beta Lactamase ◽  
Methicillin Resistant ◽  
Penicillin Binding Proteins

scholarly journals Susceptibility of Methicillin-Resistant Staphylococcus aureus to Five Quinazolinone Antibacterials

2019 ◽  
Vol 64 (1) ◽  
Author(s):  
Sara Ceballos ◽  
Choon Kim ◽  
Yuanyuan Qian ◽  
Shahriar Mobashery ◽  
Mayland Chang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Binding Proteins ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Content Type ◽  
Penicillin Binding Proteins

ABSTRACT The in vitro activities of five quinazolinone antibacterials, compounds Q1 to Q5, were tested against 210 strains of methicillin-resistant Staphylococcus aureus (MRSA). The MIC50/MIC90 values (in μg/ml) were as follows: Q1, 0.5/2; Q2, 1/4; Q3, 2/4; Q4, 0.06/0.25; and Q5, 0.125/0.5. Several strains with high MIC values (from 8 to >32 μg/ml) for some of these compounds exhibited amino acid changes in the penicillin-binding proteins, which are targeted by these antibacterials.

Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.

1997 ◽  
Vol 41 (11) ◽  
pp. 2355-2361 ◽  
Author(s):  
H Komatsuzawa ◽  
M Sugai ◽  
K Ohta ◽  
T Fujiwara ◽  
S Nakashima ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Proteins ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Insertion Mutant ◽  
Resistance Level ◽  
Methicillin Resistant ◽  
Penicillin Binding Proteins ◽  
Oxacillin Resistance ◽  
Mrsa Strains ◽  
Triton X

In methicillin-resistant Staphylococcus aureus (MRSA) strains, Triton X-100 reduced the oxacillin resistance level, although the degree of reduction varied from strain to strain. To study the responses of MRSA strains to Triton X-100, we isolated a Tn551 insertion mutant of the COL strain that became more susceptible to oxacillin in the presence of 0.02% Triton X-100. The Tn551 insertion of the mutant was transduced back to the parent strain, other MRSA strains (strains KSA8 and NCTC 10443), and methicillin-susceptible strain RN450. All transductants of MRSA strains had reduced levels of resistance to oxacillin in the presence of 0.02% Triton X-100, while those of RN450 did not. Tn551 mutants of KSA8 and NCTC 10443 also had reduced levels of resistance in the absence of 0.02% Triton X-100. The autolysis rates of the transductants in the presence of 0.02% Triton X-100 were significantly increased. Amino acid analysis of peptidoglycan and testing of heat-inactivated cells for their susceptibilities to several bacteriolytic enzymes showed that there were no significant differences between the parents and the respective Tn551 mutants. The Tn551 insertion site mapped at a location different from the previously identified fem and llm sites. Cloning and sequencing showed that Tn551 had inserted at the C-terminal region of a novel gene designated fmt. The putative Fmt protein showed a hydropathy pattern similar to that of S. aureus penicillin-binding proteins and contained two of the three conserved motifs shared by penicillin-binding proteins and beta-lactamases, suggesting that fmt may be involved in cell wall synthesis.

scholarly journals Detection of Antimicrobial Activity of Cranberry Fruit Extract against Methicillin Resistant Staphylococcus aureus and Extended Spectrum Beta Lactamase Escherichia coli

2021 ◽  
pp. 44-52
Author(s):  
Ali Nawaz Bijarani ◽  
Muhammad Owais Ismail ◽  
Zahida Memon ◽  
Faisal Afridi ◽  
Shabana Qabulio ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Activity ◽  
Beta Lactamase ◽  
Fruit Extract ◽  
Methicillin Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Fruit Extracts ◽  
Extended Spectrum

Objective: Antibacterial effects of Cranberry fruit extract on Methicillin Resistant Staphylococcus aureus and Extended Spectrum beta lactamase producing E. coli and its comparison with Linezolid and Meropenem. Materials and Methods: It is a Pre Clinical (in-vitro) study conducted in Ziauddin University from January 2020 to October 2020. All samples were collected from Ziauddin University.  All clinical samples were collected inform of pus, urine, blood, tracheal aspirations, patients admitted in surgical and medical wards, intensive care units and outdoor patients who were attending clinics. All these samples were transported to Clinical Microbiology Laboratory, Ziauddin hospital, North Nazimabad campus and then culture and sensitivity test were performed there. Sample showing double growth and contamination on agar plates were excluded from study. Results: Out of 80 samples included in this study 46(57.5%) were female and 34(42.5%) samples were male; female to male samples ratio of 1.35:1.The mean age was 45.71±11.83 years. MRSA commonly found in pus swab 15(37.5%) and 21(52.5%) ESBL producing E. coli found in urine samples. 14(35%) samples were observed anti-bacterial activity of cranberry fruit extracts against Extended Spectrum Beta Lactamase Producing Escherichia coli at 50 mg/ml concentration, followed by 10(25%) and 9(22.5%) samples at  60 mg/ml, 40 mg/ml were respectively. While resistance of Extended Spectrum Beta Lactamase Producing Escherichia coliwere observed high at different concentration level of cranberry fruit extracts. Good anti-bacterial activity of cranberry fruit extracts observed  against Methicillin Resistant Staphylococcus aureus at different                      levels of concentration 20(50%), 23(57.5%), 21(52.5%), 26(65%), 29(72.5%) samples were 20 mg/ml,30mg/ml,40mg/ml, 50mg/ml, 60mg/ml respectively. Most superior and best dose of cranberry fruit extract against Staph Aureus in about 72.5% (29) at 60mg/ml and their Comparison with linezolid and meropenem against Methicillin Resistant Staphylococcus aureus and found best positive results as compared with Linezolid and found significant p value 0.005. Conclusions: Cranberry extract has a lot of potential to prove itself to be a good antimicrobial agent. The cranberry fruit extract has high antimicrobial activity against methicillin resistant S.aureus and resistant strains of E. coli in comparison to linezolid and meropenem.

scholarly journals Increased Incidence of .BETA.-lactamase-plasmid Negative, High Level Methicillin-Resistant Staphylococcus aureus (MRSA).

1996 ◽  
Vol 43 (3) ◽  
pp. 199-206 ◽  
Author(s):  
TOSHINOBU YOKOYAMA ◽  
JUNICHI HONDA ◽  
TOMOTAKA KAWAYAMA ◽  
KATSUNARI KAJIMURA ◽  
KOTARO OIZUMI
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Beta Lactamase ◽  
Methicillin Resistant ◽  
High Level
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