scholarly journals Casein kinase II(CK-II)-Mediated Stimulation of HIV-1 Reverse Transcriptase Activity and Characterization of Selective Inhibitors in Vitro.

1999 ◽  
Vol 22 (10) ◽  
pp. 1122-1126 ◽  
Author(s):  
Shigeyoshi HARADA ◽  
Eiji HANEDA ◽  
Toshiro MAEKAWA ◽  
Yuko MORIKAWA ◽  
Shinji FUNAYAMA ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Casein Kinase Ii ◽  
Reverse Transcriptase Activity ◽  
Casein Kinase ◽  
Selective Inhibitors ◽  
Transcriptase Activity ◽  
Hiv 1 ◽  
Stimulation Of

Biochemical characterization of HIV-1 Rev as a potent activator of casein kinase II in vitro

FEBS Letters ◽  
1998 ◽  
Vol 428 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Kenzo Ohtsuki ◽  
Toshiro Maekawa ◽  
Shigeyoshi Harada ◽  
Atsushi Karino ◽  
Yuko Morikawa ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Casein Kinase Ii ◽  
Casein Kinase ◽  
Hiv 1

Characterization of Reverse Transcriptase Activity and Susceptibility to Other Nucleosides of AZT-Resistant Variants of HIV-1.

1990 ◽  
Vol 616 (1 AIDS) ◽  
pp. 346-355 ◽  
Author(s):  
MARK A. WAINBERG ◽  
MICHEL TREMBLAY ◽  
RONALD ROOKE ◽  
NORMAND BLAIN ◽  
HUGO SOUDEYNS ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Transcriptase Activity ◽  
Hiv 1

A Method for Combined Immunoaffinity Purification and Assay of HIV-1 Reverse Transcriptase Activity Useful for Crude Samples

1996 ◽  
Vol 235 (2) ◽  
pp. 141-152 ◽  
Author(s):  
J. Lennerstrand ◽  
A.-S. Rytting ◽  
C. Örvell ◽  
J.S. Gronowitz ◽  
C.F.R. Källander
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Transcriptase Activity ◽  
Immunoaffinity Purification ◽  
Hiv 1

scholarly journals Characterization of a Mutant HIV-1 Reverse Transcriptase Resistant to (+)-(5S)-4,5,6,7-tetrahydro-5-methyl-6-(3-methyl-2-butenyl)-imidazo[4,5,1-jk][1,4]benzodiazepin-2(1H)-thione (TIBO R82150)

1994 ◽  
Vol 5 (4) ◽  
pp. 278-281
Author(s):  
H. Samanta ◽  
R. Rose ◽  
A. K. Patick ◽  
C. M. Bechtold ◽  
J. Trimble ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Nucleoside Reverse Transcriptase Inhibitor ◽  
Serial Passage ◽  
Transcriptase Inhibitor ◽  
Viral Resistance ◽  
Resistant Virus ◽  
Reverse Transcriptase Inhibitor ◽  
Hiv 1

A virus strain resistant to R82150, a non-nucleoside reverse transcriptase (NNRT) inhibitor (tetrahydro-imidazo [4,5, 1- jk] [1,4] benzodiazepine-2(1 H)-thione), was isolated following serial passage of HIV-1 RF in CEM-SS cells. The virus is cross-resistant to another non-nucleoside reverse transcriptase inhibitor, TGG-II-23A [1,4-dimethyl-1-[5,5-dimethyl-2-oxazoionyl]-naphthalen-2-one), but remains susceptible to AZT, DDI, D4T and phosphonoformate (PFA). DNA sequencing of reverse transcriptase genes from resistant virus indicated that R82150 selects for amino acid alterations Y181C and V108I. In vitro mutagenized reverse transcriptase and recombinant HIV-1 (pNL4-3) carrying either of the mutations have been generated. Genotypic and phenotypic analyses identified V108I as an unreported R82150-associated mutation. Both reverse transcriptase and viral resistance assays indicated that the resistance conferred by the V108I mutation is 7-fold less than that conferred by Y181C.

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