scholarly journals Effectiveness and Toxicity Screening of Various Absorption Enhancers Using Caco-2 Cell Monolayers.

1998 ◽  
Vol 21 (6) ◽  
pp. 615-620 ◽  
Author(s):  
Ying-Shu QUAN ◽  
Koji HATTORI ◽  
Ewa LUNDBORG ◽  
Takuya FUJITA ◽  
Masahiro MURAKAMI ◽  
...  
Keyword(s):  
Toxicity Screening ◽  
Absorption Enhancers ◽  
Cell Monolayers

Biopharmaceutics: Cytotoxicity of Absorption Enhancers in Caco-2 Cell Monolayers

1998 ◽  
Vol 50 (10) ◽  
pp. 1101-1108 ◽  
Author(s):  
MICHINORI SAKAI ◽  
TERUKO IMAI ◽  
HIROSHI OHTAKE ◽  
MASAKI OTAGIRI
Keyword(s):  
Absorption Enhancers ◽  
Cell Monolayers

In Vitro Assessment of Acyclovir Permeation Across Cell Monolayers in the Presence of Absorption Enhancers

2008 ◽  
Vol 34 (3) ◽  
pp. 279-288 ◽  
Author(s):  
Pranav Shah ◽  
Viral Jogani ◽  
Pushpa Mishra ◽  
Anil Kumar Mishra ◽  
Tamishraha Bagchi ◽  
...  
Keyword(s):  
Absorption Enhancers ◽  
Cell Monolayers ◽  
In Vitro Assessment

Effects of absorption enhancers on cytoskeletal actin filaments in Caco-2 cell monolayers

Life Sciences ◽  
1998 ◽  
Vol 63 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Michinori Sakai ◽  
Teruko Imai ◽  
Hiroshi Ohtake ◽  
Hidekazu Azuma ◽  
Masaki Otagiri
Keyword(s):  
Actin Filaments ◽  
Absorption Enhancers ◽  
Cell Monolayers

scholarly journals Evaluation of the Effects of Absorption Enhancers on Caco-2 Cell Monolayers by Using a Pore Permeation Model Involving Two Different Sizes

2013 ◽  
Vol 36 (11) ◽  
pp. 1862-1866
Author(s):  
Toshinobu Seki ◽  
Airi Hamada ◽  
Yuya Egawa ◽  
Tsutomu Yamaki ◽  
Masaki Uchida ◽  
...  
Keyword(s):  
Absorption Enhancers ◽  
Cell Monolayers

Agar Diffusion Method for Toxicity Screening of Plastics on Cultured Cell Monolayers

1965 ◽  
Vol 54 (10) ◽  
pp. 1545-1547 ◽  
Author(s):  
Wallace L. Guess ◽  
S. Alan Rosenbluth ◽  
Beverly Schmidt ◽  
John Autian
Keyword(s):  
Diffusion Method ◽  
Toxicity Screening ◽  
Cultured Cell ◽  
Cell Monolayers ◽  
Agar Diffusion ◽  
Agar Diffusion Method

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

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