scholarly journals The Diabetic State Increases the Activity but Not the Number of Peritoneal Macrophages in the GK Rat Promoting the Tube Formation of Cultured Endothelial Cells in Rat Aorta.

1996 ◽  
Vol 19 (2) ◽  
pp. 199-202 ◽  
Author(s):  
Shinjiro KOBAYASHI ◽  
Bao LUO ◽  
Motonori OKABE ◽  
Ikuko KIMURA ◽  
Masayasu KIMURA
Keyword(s):  
Endothelial Cells ◽  
Peritoneal Macrophages ◽  
Rat Aorta ◽  
Tube Formation ◽  
Gk Rat ◽  
Diabetic State ◽  
Cultured Endothelial Cells

Interferon-γ-activated macrophages release interleukin-1 α to increase tube formation from endothelial cells of rat aorta

1995 ◽  
Vol 31 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Shinjiro Kobayashi ◽  
Motonori Okabe ◽  
Ikuko Kimura ◽  
Masayasu Kimura
Keyword(s):  
Endothelial Cells ◽  
Interleukin 1 ◽  
Rat Aorta ◽  
Interferon Γ ◽  
Tube Formation ◽  
Activated Macrophages

scholarly journals Exosomes secreted from osteocalcin-overexpressing endothelial progenitor cells promote endothelial cell angiogenesis

2019 ◽  
Vol 317 (5) ◽  
pp. C932-C941 ◽  
Author(s):  
Ming Yi ◽  
Ye Wu ◽  
Jun Long ◽  
Fei Liu ◽  
Zhi Liu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Rat Aorta ◽  
Tube Formation ◽  
Endothelial Progenitor ◽  
And Migration ◽  
G Protein Coupled ◽  
Receptor Family ◽  
Proliferation And Migration

Exosome secretion is an important paracrine way of endothelial progenitor cells (EPCs) to modulate resident endothelial cells. The osteocalcin (OCN)-expressing EPCs have been found to be increased in cardiovascular disease patients and are considered to be involved in the process of coronary atherosclerosis. Since OCN has been proven to prevent endothelial dysfunction, this study aimed to evaluate the effect of exosomes derived from OCN-overexpressed EPCs on endothelial cells. Exosomes derived from EPCs (Exos) and OCN-overexpressed EPCs (OCN-Exos) were isolated and incubated with rat aorta endothelial cells (RAOECs) with or without the inhibition of OCN receptor G protein-coupled receptor family C group 6 member A (GPRC6A). The effects of exosomes on the proliferation activity of endothelial cells were evaluated by CCK-8 assay, and the migration of endothelial cells was detected by wound healing assay. A tube formation assay was used to test the influence of exosomes on the angiogenesis performance of endothelial cells. Here, we presented that OCN was packed into Exos and was able to be transferred to the RAOECs via exosome incorporation, which was increased in OCN-Exos groups. Compared with Exos, OCN-Exos had better efficiency in promoting RAOEC proliferation and migration and tube formation. The promoting effects were impeded after the inhibition of GPRC6A expression in RAOECs. These data suggest that exosomes from OCN-overexpressed EPCs have a beneficial regulating effect on endothelial cells, which involved enhanced OCN-GPRC6A signaling.

scholarly journals DIFFERENCES IN MEMBRANE POTENTIAL SENSITIVITY TO POTASSIUM CHANNEL OPENERS AND HYPOXIA BETWEEN INTACT AND CULTURED ENDOTHELIAL CELLS

2009 ◽  
Vol 55 (1) ◽  
pp. 49-56
Author(s):  
A.I. Bondarenko ◽  
Keyword(s):  
Endothelial Cells ◽  
Membrane Potential ◽  
Cell Function ◽  
Cultured Cells ◽  
Rat Aorta ◽  
Resting Membrane Potential ◽  
Signaling Mechanism ◽  
Endothelial Cell Function ◽  
K Channels ◽  
Cultured Endothelial Cells

The influence of pinacidil, an activator of ATP-sensitive K+ channels, on the membrane potential of endothelial cells from intact rat aorta and cultured endothelial cells was investigated. Pinacidil evoked a slowly developing sustained hyperpolariza-tion of endothelial cells from isolated artery with the amplitude of 15±4 mV from the resting membrane potential of –4Ш мВ. In contrast, in cultured endothelial cells pinacidil was without response. Diazoxide, another activator of ATP-sensitive K+ channels, in half of the cultured cells tested, evoked a slowly developing sustained hyperpolarization with the amplitude of 3 mV. The rest of the cells studied did not respond by membrane potential changes to diazoxide. It was suggested that high sen­sitivity of the membrane potential of in situ endothelial cells to potassium channels openers may represent a potent signaling mechanism influencing endothelial cell function upon stimula­tion of vascular KATP channels.

High-glucose--triggered apoptosis in cultured endothelial cells

Diabetes ◽  
1995 ◽  
Vol 44 (11) ◽  
pp. 1323-1327 ◽  
Author(s):  
S. M. Baumgartner-Parzer ◽  
L. Wagner ◽  
M. Pettermann ◽  
J. Grillari ◽  
A. Gessl ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Cultured Endothelial Cells

Advanced glycation end product-induced activation of NF-kappaB is suppressed by alpha-lipoic acid in cultured endothelial cells

Diabetes ◽  
1997 ◽  
Vol 46 (9) ◽  
pp. 1481-1490 ◽  
Author(s):  
A. Bierhaus ◽  
S. Chevion ◽  
M. Chevion ◽  
M. Hofmann ◽  
P. Quehenberger ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lipoic Acid ◽  
Alpha Lipoic Acid ◽  
Advanced Glycation ◽  
Advanced Glycation End Product ◽  
Cultured Endothelial Cells ◽  
Nf Kappab

PERSPECTIVES OF APPLICATION OF ANTIBODIES AGAINST ENDOGLIN (CD105) FOR VISUALIZATION AND ANTI-ANGIOGENE THERAPY OF TUMORS

2018 ◽  
Vol 64 (4) ◽  
pp. 504-507
Author(s):  
Vladimir Klimovich ◽  
Natalya Vartanyan ◽  
Anastasiya Stolbovaya ◽  
Lidiya Terekhina ◽  
Olga Shashkova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Vascular Endothelium ◽  
Immune Complexes ◽  
Targeted Delivery ◽  
Biological Fluids ◽  
Therapeutic Drugs ◽  
Cultured Endothelial Cells ◽  
Hybridoma Technology

During last years monoclonal antibodies (MAB) directed against vascular endothelium markers demonstrated their efficiency for visualization and targeted delivery of therapeutic drugs to tumors. Endoglin (CD105) which serves as a key element that determines endothelial cells quiescence or activation is one of such markers. Endoglin is highly expressed on the vascular endothelium of growing tumors. A first panel of MAB against endoglin in our country was produced at the hybridoma technology laboratory of RRC RST named after A.M. Granov. On the basis of these MAB ELISA was created allowing detection of endoglin in human plasma and other biological fluids. Several MAB had been shown to bind endoglin on the membrane of the cultured endothelial cells and to persist there for several hours. During the first 30 min after binding some of the immune complexes “endoglin-MAB” were internalized into the cytoplasm and were found included in the endosomes. In future these MAB can be used to create the reagents for the addressed delivery of isotope tags both on the membrane and into the cytoplasm of endothelial cells.

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