scholarly journals Evaluation of Skin Damage of Cyclic Monoterpenes, Percutaneous Absorption Enhancers, by Using Cultured Human Skin Cells.

1993 ◽  
Vol 16 (9) ◽  
pp. 912-916 ◽  
Author(s):  
Megumi KITAHARA ◽  
Fumiko ISHIGURO ◽  
Kozo TAKAYAMA ◽  
Koichi ISOWA ◽  
Tsuneji NAGAI
Keyword(s):  
Human Skin ◽  
Percutaneous Absorption ◽  
Skin Damage ◽  
Absorption Enhancers ◽  
Skin Cells ◽  
Human Skin Cells

Human Skin in Organ Culture and Human Skin Cells (Keratinocytes and Fibroblasts) in Monolayer Culture for Assessment of Chemically Induced Skin Damage

2007 ◽  
Vol 35 (5) ◽  
pp. 693-701 ◽  
Author(s):  
James Varani ◽  
Patricia Perone ◽  
Diana M. Spahlinger ◽  
Lisa M. Singer ◽  
Kelly L. Diegel ◽  
...  
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Monolayer Culture ◽  
Skin Damage ◽  
Skin Cells ◽  
Chemically Induced ◽  
Human Skin Cells

scholarly journals Differential expression of selenoproteins by human skin cells and protection by selenium from UVB-radiation-induced cell death

1998 ◽  
Vol 332 (1) ◽  
pp. 231-236 ◽  
Author(s):  
Teresa S. RAFFERTY ◽  
Roderick C. McKENZIE ◽  
John A. A. HUNTER ◽  
A. Forbes HOWIE ◽  
John R. ARTHUR ◽  
...  
Keyword(s):  
Cell Death ◽  
Human Skin ◽  
Sodium Selenite ◽  
Human Fibroblasts ◽  
Human Keratinocytes ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Skin Cells ◽  
Radiation Induced ◽  
Human Skin Cells

The generation of reactive oxygen species has been implicated as part of the mechanism responsible for UVB-radiation-induced skin damage. In mice, evidence suggests that increased dietary selenium intake may protect skin from many of the harmful effects of UVB radiation. We sought to determine the selenoprotein profile of cultured human skin cells and whether selenium supplementation could protect keratinocytes and melanocytes from the lethal effects of UVB radiation. Labelling experiments using [75Se]selenite showed qualitative and quantitative differences in selenoprotein expression by human fibroblasts, keratinocytes and melanocytes. This was most noticeable for thioredoxin reductase (60 kDa) and phospholipid glutathione peroxidase (21 kDa); these proteins were identified by Western blotting. Despite these differences, we found that a 24 h preincubation with sodium selenite or selenomethionine protected both cultured human keratinocytes and melanocytes from UVB-induced cell death. With primary keratinocytes, the greatest reduction in cell death was found with 10 nM sodium selenite (79% cell death reduced to 21.7%; P< 0.01) and with 50 nM selenomethionine (79% cell death reduced to 13.2%; P< 0.01). Protection could be obtained with concentrations as low as 1 nM with sodium selenite and 10 nM with selenomethionine. When selenium was added after UVB radiation, little protection could be achieved, with cell death only being reduced from 88.5% to about 50% with both compounds. In all of the experiments sodium selenite was more potent than selenomethionine at providing protection from UVB radiation.

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

Basic Red 51, a permitted semi-permanent hair dye, is cytotoxic to human skin cells: Studies in monolayer and 3D skin model using human keratinocytes (HaCaT)

2014 ◽  
Vol 227 (2) ◽  
pp. 139-149 ◽  
Author(s):  
Thalita B. Zanoni ◽  
Manoela Tiago ◽  
Fernanda Faião-Flores ◽  
Silvia B. de Moraes Barros ◽  
Aalt Bast ◽  
...  
Keyword(s):  
Human Skin ◽  
Human Keratinocytes ◽  
Skin Model ◽  
Hair Dye ◽  
Skin Cells ◽  
Human Skin Cells ◽  
3D Skin

scholarly journals Effects of a complex mixture prepared from agrimonia, houttuynia, licorice, peony, and phellodendron on human skin cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kyung-Ha Lee ◽  
Jeong Pyo Lee ◽  
Wanil Kim
Keyword(s):  
Human Skin ◽  
Complex Mixture ◽  
Skin Barrier ◽  
Aging Effect ◽  
Human Keratinocytes ◽  
Skin Barrier Function ◽  
Active Ingredients ◽  
Γ Irradiation ◽  
Skin Cells ◽  
Human Skin Cells

AbstractActive ingredients derived from natural sources are widely utilized in many industries. Cosmetic active ingredients are largely derived from various plants. In this study, we examined whether a mixture of plant extracts obtained from agrimonia, houttuynia, licorice, peony, and phellodendron (hereafter AHLPP), which are well-known for their effects on skin, could affect skin barrier function, inflammation, and aging in human skin cells. We also determined whether AHLPP extracts sterilized using γ-irradiation (to avoid preservatives) retained their skin cell regulating activity. The AHLPP mixture could downregulate representative pro-inflammatory cytokines including IL 1-β and IL 7. Procollagen peptide synthesis was also increased by AHLPP treatment along with mRNA upregulation of barrier proteins such as filaggrin and desmoplakin. The AHLPP mixture showed an anti-aging effect by significantly upregulating telomerase activity in human keratinocytes. We further observed TERT upregulation and CDKN1B downregulation, implying a weakening of pro-aging signal transduction. Co-cultivation of a hydrogel polymer containing the AHLPP mixture with human skin cells showed an alteration in skin-significant genes such as FLG, which encodes filaggrin. Thus, the AHLPP mixture with or without γ-irradiation can be utilized for skin protection as it alters the expression of some significant genes in human skin cells.

Neuropeptides and skin aging

2013 ◽  
Vol 16 (1) ◽  
Author(s):  
Rana Elewa ◽  
Evgenia Makrantonaki ◽  
Christos C. Zouboulis
Keyword(s):  
Human Skin ◽  
Inflammatory Cytokines ◽  
Vasoactive Intestinal Polypeptide ◽  
Skin Aging ◽  
Target Cells ◽  
Hormone Metabolism ◽  
Skin Cells ◽  
Calcitonin Gene ◽  
Aged Skin ◽  
Human Skin Cells

AbstractNeuropeptides (NP) are peptides that are released as chemical messengers from nerve cells. They act either in an endocrine manner, where they reach their target cells via the bloodstream or a paracrine manner, as co-transmitters modulating the function of neurotransmitters. To date approximately 100 different NP have been described in the literature. In recent years, several studies have documented that human skin expresses several functional receptors for NP, such as corticotropin-releasing hormone, melanocortins, β-endorphin, vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene-related peptide. These receptors modulate the production of inflammatory cytokines, proliferation, differentiation, lipogenesis and hormone metabolism in human skin cells. In addition, several NP are directly produced by human skin cells, indicating the complexity of understanding the real functions of NPs in human skin. In this review we address the possible effects of neuropeptides on the pathogenesis of aged skin.

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