scholarly journals Increase in Lipoprotein Lipase Activity in Isolated Rat Adipose Tissue by Selenate.

1993 ◽  
Vol 16 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Hiroshi UEKI ◽  
Yusuke OHKURA ◽  
Toshio MOTOYASHIKI ◽  
Nobuaki TOMINAGA ◽  
Tetsuo MORITA
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Rat Adipose Tissue ◽  
Isolated Rat

Stimulation of lipoprotein lipase activity of rat adipose tissue and post-heparin plasma by N 6 -(phenylisopropyl)adenosine

FEBS Letters ◽  
1981 ◽  
Vol 132 (1) ◽  
pp. 121-123 ◽  
Author(s):  
Jorma J. Ohisalo ◽  
Håkan Strandberg ◽  
Ella Kostiainen ◽  
Timo Kuusi ◽  
Christian Ehnholm
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Heparin Plasma ◽  
Rat Adipose Tissue ◽  
Phenylisopropyl Adenosine ◽  
Stimulation Of

scholarly journals Increased lipoprotein lipase content in the adipose tissue of suckling and weaning obese Zucker rats

1988 ◽  
Vol 249 (1) ◽  
pp. 45-49 ◽  
Author(s):  
I Dugail ◽  
A Quignard-Boulange ◽  
L Brigant ◽  
J Etienne ◽  
L Noe ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Catalytic Properties ◽  
Acetone Extract ◽  
Zucker Rats ◽  
Lipoprotein Lipase Activity ◽  
Obese Zucker Rats ◽  
Rat Adipose Tissue ◽  
Fat Pads

The aim of this study was to determine whether the increase in lipoprotein lipase activity displayed by the adipose tissue of obese (fa/fa) rats as compared with that of lean (Fa/fa) rats could be ascribed to a change in the content or in the catalytic properties of the enzyme. The question was addressed in rats of two ages: in 7-day-old suckling and in 30-day-old post-weaning pups. Inguinal fat-pads were removed surgically (7 days of age) or after killing (30 days of age), and acetone-extract powders were prepared. The relative quantity of enzyme was assessed by immunotitration using an antiserum raised in goat against purified lipoprotein lipase from rat adipose tissue. The results indicate that increases in enzyme activity in obese animals were strictly paralleled by increases in the amount of enzyme in suckling as well as in post-weaning pups. Moreover, the apparent Km values of lipoprotein lipase for its substrate triacylglycerol were identical in the two genotypes. In conclusion, the genotype-mediated increase in lipoprotein lipase activity in adipose tissue of obese Zucker rats was fully accounted for by an increase in the content of the enzyme. In addition, this work documents the mechanism of the increase in lipoprotein lipase activity during weaning, which is mediated mainly through changes in the adipose-tissue enzyme content.

scholarly journals Effects of insulin, glucocorticoids and adrenaline on the activity of rat adipose-tissue lipoprotein lipase

1980 ◽  
Vol 188 (1) ◽  
pp. 185-192 ◽  
Author(s):  
P Ashby ◽  
D S Robinson
Keyword(s):  
Protein Synthesis ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Fat Bodies ◽  
Rat Adipose Tissue ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase activity of epididymal fat-bodies from starved rats was measured during incubations at 37 degrees C in vitro. Protein synthesis independent activation of the enzyme, previously observed during incubations at 25 decrease C, also occurs at 37 degrees C. Protein-synthesis-dependent increases in the activity of the enzyme occur in the presence of insulin and are markedly potentiated by glucocorticoids. The effects on the activity of the enzyme of insulin alone, or in the presence of glucocorticoids, are correlated with its effects on total protein synthesis in the tissue. Adrenaline antagonizes the increase in activity of the enzyme brought about by insulin and abolishes the potentiation of insulin action by glucocorticoids. These changes may be due, at least in part, to its stimulation of inactivation of the enzyme in the tissue. It is suggested that changes in adipose-tissue lipoprotein lipase activity that occur with changes in nutritional status in vivo result from the combined effects of changes in plasma insulin and glucocorticoid concentrations.

scholarly journals Post-translational regulation of lipoprotein lipase activity in adipose tissue

1978 ◽  
Vol 176 (3) ◽  
pp. 865-872 ◽  
Author(s):  
P Ashby ◽  
D P Bennett ◽  
I M Spencer ◽  
D S Robinson
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Translational Regulation ◽  
Progressive Increase ◽  
Lipoprotein Lipase Activity ◽  
Dependent Part ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Energy Dependent

Changes in adipose-tissue lipoprotein lipase activity that are independent of protein synthesis were investigated in an incubation system in vitro. Under appropriate conditions at 25 degrees C a progressive increase in the enzyme activity occurs that is energy-dependent. Part of the enzyme is rapidly inactivated when the tissue is incubated with adrenaline or adrenaline plus theophylline. The mechanism of this inactivation appears to be distinct from, and to follow, the activation of the enzyme. A hypothesis is presented to account for the results in terms of an activation of the enzyme during obligatory post-translational processing and a catecholamine-regulated inactivation of the enzyme as an alternative to secretion from the adipocyte.

Sign in / Sign up

Export Citation Format

Share Document