scholarly journals Inhibitory Effect of .BETA.-Glucans on Zymosan-Mediated Hydrogen Peroxide Production by Murine Peritoneal Macrophages in Vitro.

1993 ◽  
Vol 16 (5) ◽  
pp. 462-467 ◽  
Author(s):  
Yoshiyuki ADACHI ◽  
Naohito OHNO ◽  
Toshiro YADOMAE
Keyword(s):  
Hydrogen Peroxide ◽  
Inhibitory Effect ◽  
Peritoneal Macrophages ◽  
Hydrogen Peroxide Production ◽  
Beta Glucans ◽  
Murine Peritoneal Macrophages

Inhibitory effects of antithyroid drugs on oxygen radical formation in human neutrophils

1986 ◽  
Vol 112 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Minoru Imamura ◽  
Norihiko Aoki ◽  
Takuya Saito ◽  
Yasuhiro Ohno ◽  
Yoshiyuki Maruyama ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Inhibitory Effect ◽  
Neutrophil Function ◽  
Oxygen Radical ◽  
Radical Formation ◽  
Antithyroid Drugs ◽  
Human Neutrophils ◽  
Inhibitory Effects ◽  
Hydrogen Peroxide Production

Abstract. The effects of antithyroid drugs and related agents on human neutrophil function were studied. Neutrophil function was mainly assessed through oxygen radical formation as determined by chemiluminescence (CL) response, superoxide anion (O−2) generation and hydrogen peroxide production. Propylthiouracil (PTU) at a therapeutic concentration (10 μg/ml) inhibited CL response evoked by phorbol myristate acetate (PMA) and other stimulators. The inhibitory effect was not enhanced by pre-incubation of neutrophils with PTU and not exerted through a direct cytotoxic effect of the drug. It was not related to the kind of stimulators to evoke CL response in neutrophils either. However, the inhibitory effect disappeared when PTU was removed from the reaction mixture for CL response. PTU did not inhibit O−2 generation but markedly inhibited hydrogen peroxide production in neutrophils or activity of hydrogen peroxide in vitro. Morphologically, the unique change of cellular configuration of chemotactic neutrophils caused by N-formyl-methionylleucyl-phenylalanine (FMLP) was not influenced with PTU. Since hydrogen peroxide is mainly derived from O−2, these observations suggest that PTU may have a scavenger effect on hydrogen peroxide activity. Inhibition of CL response in neutrophils was also demonstrated with methimazole (MMI), thiouracil and thiourea, but not with imidazole and uracil, which suggests that their inhibitory effect on CL response in neutrophils may be closely related to the antithyroid activity.

Immunocytochemical Analysis of Murine Peritoneal Macrophages Treated with “Poly-Plat”, an in Vitro and in Vivo Study

1998 ◽  
Vol 4 (S2) ◽  
pp. 1122-1123
Author(s):  
H. J. Muenchen ◽  
S.K. Aggarwal
Keyword(s):  
Immune System ◽  
Mechanism Of Action ◽  
Second Generation ◽  
Peritoneal Macrophages ◽  
In Vivo Study ◽  
Immunocytochemical Analysis ◽  
Murine Peritoneal Macrophages

Poly-[(trans-1,2-diaminocyclohexane) platinumj-carboxyamylose (“poly-plat“) is a second generation analog of cisplatin which enhances the immune system with greater efficacy in vitro and in vivo “Poly-plat” contains 1/5 the platinum of CDDP and demonstrates less toxicity. In order to understand the mechanism of action of this compound an in vitro and in vivo study was performed. Swiss Webster mice and isolated murine peritoneal macrophages were treated with “poly-plat” (10 mg/kg). The Swiss Webster mice were given bolus injections and sacrificed at 2 and 12 days. Peritoneal macrophages were then isolated and allowed to incubate in culture for 24 h. Peritoneal macrophages were also isolated from normal mice and treated with the drugs for 2 h. After treatments the macrophages were placed in fresh media and allowed to incubate 24 h. Supematants were isolated at various times during culture for immunocytochemical analysis.Both in vitro and in vivo studies showed enhanced immunostimulation after their respective treatments.

scholarly journals Comparison between Peritoneal Macrophage Activation by Bougainvillea xbuttiana Extract and LPS and/or Interleukins

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Lluvia Arteaga Figueroa ◽  
Rodolfo Abarca-Vargas ◽  
Claudia García Alanis ◽  
Vera L. Petricevich
Keyword(s):  
Hydrogen Peroxide ◽  
Cytotoxic Effect ◽  
Macrophage Activation ◽  
Peritoneal Macrophages ◽  
Immunomodulatory Activity ◽  
Hydrogen Peroxide Production ◽  
Griess Reagent ◽  
Violet Crystal ◽  
Low Cytotoxicity

Activation of macrophages may be one of the possible approaches in modulating inflammation. We previously reported that Bougainvillea xbuttiana extract showed an immunomodulatory activity. Here we compare the activation of macrophages exposed to B. xbuttiana extract and compare it with the other treatments such as LPS, IL-4, and IL-10. The cytotoxic effect of extract on peritoneal macrophages was determined by the technique of violet crystal staining. To verify the activation of macrophages we used the tests of vacuolization, hydrogen peroxide production, and percentages of cellular expansion and phagocytosis. The levels of interleukins secreted by macrophages treated with the extract, LPS, and cytokines were determined by the biological assay for the determination of TNF levels and by ELISA for all other interleukins. NO levels were evaluated by colorimetric reactions using Griess reagent. Our results showed that B. xbuttiana extract induced (a) low cytotoxicity percentages, (b) increased vacuolization, hydrogen peroxide production and cell expansion and phagocytosis percentages, and (c) decreased production of TNF-α, IFN-γ, IL-1β, and IL-6 and potentiated production of IL-4, IL-10 and TGF-β. These results suggest that B. xbuttiana extract was able to activate the murine macrophages in a manner similar to those macrophages exposed to IL-4 and IL-10.

scholarly journals Interspecies Inhibition ofPorphyromonas gingivalisby Yogurt-DerivedLactobacillus delbrueckiiRequires Active Pyruvate Oxidase

2019 ◽  
Vol 85 (18) ◽  
Author(s):  
Louis P. Cornacchione ◽  
Brian A. Klein ◽  
Margaret J. Duncan ◽  
Linden T. Hu
Keyword(s):  
Hydrogen Peroxide ◽  
Periodontal Disease ◽  
Microbial Interactions ◽  
Lactobacillus Delbrueckii ◽  
Species Selection ◽  
Probiotic Bacteria ◽  
Pyruvate Oxidase ◽  
Hydrogen Peroxide Production ◽  
Content Type

ABSTRACTDespite a growing interest in using probiotic microorganisms to prevent disease, the mechanisms by which probiotics exert their action require further investigation.Porphyromonas gingivalisis an important pathogen implicated in the development of periodontitis. We isolated several strains ofLactobacillus delbrueckiifrom dairy products and examined their ability to inhibitP. gingivalisgrowthin vitro. We observed strain-specific inhibition ofP. gingivalisgrowthin vitro. Whole-genome sequencing of inhibitory and noninhibitory strains ofL. delbrueckiirevealed significant genetic differences supporting the strain specificity of the interaction. Extracts of theL. delbrueckiiSTYM1 inhibitory strain contain inhibitory activity that is abolished by treatment with heat, proteinase K, catalase, and sodium sulfite. We purified the inhibitory protein(s) fromL. delbrueckiiSTYM1 extracts using ammonium sulfate precipitation, anion-exchange chromatography, and gel filtration chromatography. Pyruvate oxidase was highly enriched in the purified samples. Lastly, we showed that purified, catalytically active, recombinant pyruvate oxidase is sufficient to inhibitP. gingivalisgrowthin vitrowithout the addition of cofactors. Further, using a saturated transposon library, we isolated transposon mutants ofP. gingivalisin thefeoB2(PG_1294) gene that are resistant to killing by inhibitoryL. delbrueckii, consistent with a mechanism of hydrogen peroxide production by pyruvate oxidase. Our results support the current understanding of the importance of strain selection, not simply species selection, in microbial interactions. SpecificL. delbrueckiistrains or their products may be effective in the treatment and prevention ofP. gingivalis-associated periodontal disease.IMPORTANCEP. gingivalisis implicated in the onset and progression of periodontal disease and associated with some systemic diseases. Probiotic bacteria represent an attractive preventative therapy for periodontal disease. However, the efficacy of probiotic bacteria can be variable between studies. Our data support the known importance of selecting particular strains of bacteria for probiotic use, not simply a single species. Specifically, in the context of probiotic intervention of periodontitis, our data suggest that high-level expression of pyruvate oxidase with hydrogen peroxide production inL. delbrueckiicould be an important characteristic for the design of a probiotic supplement or a microbial therapeutic.

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