scholarly journals Effect of the local anesthetic dibucaine on the surface membrane in sterol-manipulated Tetrahymena pyriformis studied by freeze-fracture electron microscopy.

1984 ◽  
Vol 9 (4) ◽  
pp. 381-389
Author(s):  
Takashi Sekiya ◽  
Yoshinori Nozawa
Keyword(s):  
Electron Microscopy ◽  
Local Anesthetic ◽  
Surface Membrane ◽  
Freeze Fracture ◽  
Tetrahymena Pyriformis ◽  
Freeze Fracture Electron Microscopy ◽  
Fracture Electron

scholarly journals Freeze-fracture Electron Microscopy on Microbubbles Used as Theranostics or Made of Lung Surfactant

2010 ◽  
Vol 16 (S2) ◽  
pp. 1172-1173
Author(s):  
B Papahadjopoulos-Sternberg ◽  
J Ackrell
Keyword(s):  
Electron Microscopy ◽  
Lung Surfactant ◽  
Freeze Fracture ◽  
Freeze Fracture Electron Microscopy ◽  
Fracture Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

Ultrastructural analysis of the apical ectodermal ridge during vertebrate limb morphogenesis

Development ◽  
1977 ◽  
Vol 41 (1) ◽  
pp. 223-232
Author(s):  
John F. Fallon ◽  
Robert O. Kelley
Keyword(s):  
Electron Microscopy ◽  
Gap Junctions ◽  
Freeze Fracture ◽  
Apical Ectodermal Ridge ◽  
Structural Requirements ◽  
Limb Morphogenesis ◽  
Vertebrate Limb ◽  
The Difference ◽  
Freeze Fracture Electron Microscopy ◽  
Fracture Electron

The fine structure of the apical ectodermal ridge of five phylogenetically divergent orders of mammals and two orders of birds was examined using transmission and freeze fracture electron microscopy. Numerous large gap junctions were found in all apical ectodermal ridges studied. This was in contrast to the dorsal and ventral limb ectoderms where gap junctions were always very small and sparsely distributed. Thus, gap junctions distinguish the inductively active apical epithelium from the adjacent dorsal and ventral ectoderms. The distribution of gap junctions in the ridge was different between birds and mammals but characteristic within the two classes. Birds, with a pseudostratified columnar apical ridge, had the heaviest concentration of gap junctions at the base of each ridge cell close to the point where contact was made with the basal lamina. Whereas mammals, with a stratified cuboidal to squamous apical ridge, had a more uniform distribution of gap junctions throughout the apical epithelium. The difference in distribution for each class may reflect structural requirements for coupling of cells in the entire ridge. We propose that all cells of the apical ridges of birds and mammals are electrotonically and/or metabolically coupled and that this may be a requirement for the integrated function of the ridge during limb morphogenesis.

Freeze-fracture electron microscopy: relationship of membrane structural features to transport physiology

1977 ◽  
Vol 232 (2) ◽  
pp. F77-F83 ◽  
Author(s):  
J. B. Wade ◽  
W. A. Kachadorian ◽  
V. A. DiScala
Keyword(s):  
Electron Microscopy ◽  
Freeze Fracture ◽  
Structural Features ◽  
Toad Urinary Bladder ◽  
Luminal Membrane ◽  
Cautious Interpretation ◽  
Freeze Fracture Electron Microscopy ◽  
Induced Changes ◽  
Relationship Of ◽  
Fracture Electron

Recent observations utilizing freeze-fracturing electron microscopy are discussed which indicate that the membrane structural features visualized by this technique may in some instances be related to specialized membrane transport properties. The occurrence of organized aggregates of intramembrane particles observed in vasopressin or cAMP-treated toad urinary bladder has been found to be closely correlated with induced changes in the permeability of the luminal membrane. Although a cautious interpretation is considered appropriate, these observations raise the possibility that some aspects of hormone action may be restricted to limited regions of membrane. Difficulties in interpretation and some serious limitations of the freeze-fracture technique are discussed.

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