scholarly journals Identification and Characterization of Two Flavohemoglobin Genes in Dictyostelium discoideum.

2000 ◽  
Vol 25 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Miho Iijima ◽  
Hajime Shimizu ◽  
Yoshimasa Tanaka ◽  
Hideko Urushihara
Keyword(s):  
Dictyostelium Discoideum ◽  
Identification And Characterization

scholarly journals Characterization of TbPDE2A, a Novel Cyclic Nucleotide-specific Phosphodiesterase from the Protozoan ParasiteTrypanosoma brucei

2000 ◽  
Vol 276 (15) ◽  
pp. 11559-11566 ◽  
Author(s):  
Roya Zoraghi ◽  
Stefan Kunz ◽  
Kewei Gong ◽  
Thomas Seebeck
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Trypanosoma Brucei ◽  
Dictyostelium Discoideum ◽  
Cyclic Nucleotide ◽  
Catalytic Domain ◽  
Sequence Identity ◽  
Essential Enzyme ◽  
Identification And Characterization

This study reports the identification and characterization of a cAMP-specific phosphodiesterase from the parasitic hemoflagellateTrypanosoma brucei. TbPDE2A is a class I phosphodiesterase. Its catalytic domain exhibits 30–40% sequence identity with those of all 11 mammalian phosphodiesterase (PDE) families, as well as withPDE2fromSaccharomyces cerevisiae,duncefromDrosophila melanogaster, andregAfromDictyostelium discoideum. The overall structure of TbPDE2A resembles that of human PDE11A in that its N-terminal region contains a single GAF domain. This domain is very similar to those of the mammalian PDE2, -5, -6, -10, and -11, where it constitutes a potential cGMP binding site. TbPDE2A can be expressed inS. cerevisiae, and it complements anS. cerevisiaePDE deletion strain. Recombinant TbPDE2A is specific for cAMP, with aKmof ∼2 μm. It is entirely resistant to the nonselective PDE inhibitor 3-isobutyl-1-methylxanthine, but it is sensitive to trequinsin, dipyridamole, sildenafil, and ethaverine with IC50values of 5.4, 5.9, 9.4, and 14.2 μm, respectively. All four compounds inhibit proliferation of bloodstream form trypanosomes in culture, indicating that TbPDE2A is an essential enzyme.

Identification and Characterization of Two Penta-EF-Hand Ca2+-Binding Proteins in Dictyostelium discoideum

2001 ◽  
Vol 130 (2) ◽  
pp. 207-215 ◽  
Author(s):  
S. Ohkouchi ◽  
K. Nishio ◽  
M. Maeda ◽  
K. Hitomi ◽  
H. Adachi ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Binding Proteins ◽  
Ef Hand ◽  
Identification And Characterization

scholarly journals Identification and characterization of peptide: N- glycanase from Dictyostelium discoideum

2012 ◽  
Vol 13 (1) ◽  
pp. 9 ◽  
Author(s):  
Anuradha Gosain ◽  
Rakhee Lohia ◽  
Anju Shrivastava ◽  
Shweta Saran
Keyword(s):  
Dictyostelium Discoideum ◽  
Identification And Characterization
Sign in / Sign up

Export Citation Format

Share Document