scholarly journals Characterization of Cytoplasmic Dynein Light-intermediate Chain isoforms in Rat Testis.

1998 ◽  
Vol 23 (3) ◽  
pp. 169-178 ◽  
Author(s):  
Seishi Maeda ◽  
Sang-Yoon Nam ◽  
Masahiko Fujisawa ◽  
Nobuaki Nakamuta ◽  
Kenji Ogawa ◽  
...  
Keyword(s):  
Cytoplasmic Dynein ◽  
Rat Testis ◽  
Intermediate Chain

scholarly journals Localization of Cytoplasmic Dynein Light-intermediate Chain mRNA in the Rat Testis Using In Situ Hybridization.

1998 ◽  
Vol 23 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Seishi Maeda ◽  
Sang-Yoon Nam ◽  
Masahiko Fujisawa ◽  
Nobuaki Nakamuta ◽  
Kenji Ogawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Cytoplasmic Dynein ◽  
Rat Testis ◽  
Intermediate Chain

scholarly journals Structural and thermodynamic characterization of a cytoplasmic dynein light chain intermediate chain complex

2007 ◽  
Vol 104 (24) ◽  
pp. 10028-10033 ◽  
Author(s):  
J. C. Williams ◽  
P. L. Roulhac ◽  
A. G. Roy ◽  
R. B. Vallee ◽  
M. C. Fitzgerald ◽  
...  
Keyword(s):  
Light Chain ◽  
Chain Complex ◽  
Cytoplasmic Dynein ◽  
Dynein Light Chain ◽  
Thermodynamic Characterization ◽  
Intermediate Chain

Cloning and Characterization of Two Cytoplasmic Dynein Intermediate Chain Genes in Mouse and Human

Genomics ◽  
1999 ◽  
Vol 55 (3) ◽  
pp. 257-267 ◽  
Author(s):  
Michael A. Crackower ◽  
David S. Sinasac ◽  
James Xia ◽  
Jun Motoyama ◽  
Michal Prochazka ◽  
...  
Keyword(s):  
Cytoplasmic Dynein ◽  
Dynein Intermediate Chain ◽  
Intermediate Chain

scholarly journals Isolation and characterization of TH3, a germ cell-specific variant of histone 3 in rat testis.

1984 ◽  
Vol 259 (14) ◽  
pp. 8769-8776
Author(s):  
P K Trostle-Weige ◽  
M L Meistrich ◽  
W A Brock ◽  
K Nishioka
Keyword(s):  
Germ Cell ◽  
Rat Testis ◽  
Histone 3 ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of TH2A, a germ cell-specific variant of histone 2A in rat testis.

1982 ◽  
Vol 257 (10) ◽  
pp. 5560-5567
Author(s):  
P K Trostle-Weige ◽  
M L Meistrich ◽  
W A Brock ◽  
K Nishioka ◽  
J W Bremer
Keyword(s):  
Germ Cell ◽  
Rat Testis ◽  
Isolation And Characterization

scholarly journals Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster.

1996 ◽  
Vol 16 (5) ◽  
pp. 1966-1977 ◽  
Author(s):  
T Dick ◽  
K Ray ◽  
H K Salz ◽  
W Chia
Keyword(s):  
Drosophila Melanogaster ◽  
Light Chain ◽  
Blot Analysis ◽  
Cytoplasmic Dynein ◽  
P Element ◽  
Female Sterility ◽  
Dynein Light Chain ◽  
Loss Of Function ◽  
Light Chain Gene

We report the molecular and genetic characterization of the cytoplasmic dynein light-chain gene, ddlc1, from Drosophila melanogaster. ddlc1 encodes the first cytoplasmic dynein light chain identified, and its genetic analysis represents the first in vivo characterization of cytoplasmic dynein function in higher eucaryotes. The ddlc1 gene maps to 4E1-2 and encodes an 89-amino-acid polypeptide with a high similarity to the axonemal 8-kDa outer-arm dynein light chain from Chlamydomonas flagella. Developmental Northern (RNA) blot analysis and ovary and embryo RNA in situ hybridizations indicate that the ddlc1 gene is expressed ubiquitously. Anti-DDLC1 antibody analyses show that the DDLC1 protein is localized in the cytoplasm. P-element-induced partial-loss-of-function mutations cause pleiotropic morphogenetic defects in bristle and wing development, as well as in oogenesis, and hence result in female sterility. The morphological abnormalities found in the ovaries are always associated with a loss of cellular shape and structure, as visualized by a disorganization of the actin cytoskeleton. Total-loss-of-function mutations cause lethality. A large proportion of mutant animals degenerate during embryogenesis, and the dying cells show morphological changes characteristic of apoptosis, namely, cell and nuclear condensation and fragmentation, as well as DNA degradation. Cloning of the human homolog of the ddlc1 gene, hdlc1, demonstrates that the dynein light-chain 1 is highly conserved in flies and humans. Northern blot analysis and epitope tagging show that the hdlc1 gene is ubiquitously expressed and that the human dynein light chain 1 is localized in the cytoplasm. hdlc1 maps to 14q24.

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