scholarly journals SEL1L Is Required for Endoplasmic Reticulum-associated Degradation of Misfolded Luminal Proteins but not Transmembrane Proteins in Chicken DT40 Cell Line

2011 ◽  
Vol 36 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Satoshi Ninagawa ◽  
Tetsuya Okada ◽  
Shunichi Takeda ◽  
Kazutoshi Mori
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Line ◽  
Transmembrane Proteins ◽  
Dt40 Cell ◽  
Endoplasmic Reticulum Associated Degradation ◽  
Chicken Dt40 Cell

scholarly journals Validation of the Reference Genes for the Gene Expression Studies in Chicken DT40 Cell Line

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 372
Author(s):  
Aleksandra Dunislawska ◽  
Anna Slawinska ◽  
Maria Siwek
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Reference Gene ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Relative Gene Expression ◽  
Dt40 Cell ◽  
Relative Gene ◽  
Chicken Dt40 Cell ◽  
Selection Of

The selection of a suitable reference gene assures a reliable gene expression analysis when using the qPCR method. Normalization of the reaction is based on the basic metabolism genes. These genes show a constant, unregulated expression in all cells and function throughout their lifetime. In the current study, seven reference gene candidates were screened using RT-qPCR, to determine the best-matched pair of reference genes in the chicken DT40 cell line. The DT40 was derived from bursal lymphoma cells that were subjected to RAV-1 bird retroviral infection. It is a simplified in vitro model that allows tracking the direct interaction of stimulants on the lymphoid population and profiling of the hepatocellular B cell transcriptome. The reference gene analysis was carried out using statistical tools integrating four independent methods—geNorm, Best Keeper, NormFinder, delta Ct and RefFinder. Based on the selected reference genes, the relative gene expression analysis was done using the ddCt method. Complete relative gene expression study on a panel of the target genes revealed that proper selection of reference genes depending on the tissue eliminate decreases in data quality. The SDHA and RPL4 genes constitute stable internal controls as reference genes when analyzing gene expression in the DT40 cell line.

scholarly journals Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction

2013 ◽  
Vol 77 (4) ◽  
pp. 228-233 ◽  
Author(s):  
Eiko N. Minakawa ◽  
Hodaka Yamakado ◽  
Atsushi Tanaka ◽  
Kengo Uemura ◽  
Shunichi Takeda ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Mitochondrial Dysfunction ◽  
Cell Line ◽  
Dt40 Cell ◽  
Familial Parkinson’S Disease ◽  
Chicken Dt40 Cell

DNA Repair Studies: Experimental Evidence in Support of Chicken DT40 Cell Line as a Unique Model

2001 ◽  
Vol 20 (4) ◽  
pp. 11 ◽  
Author(s):  
Pawan Kumar Dhar ◽  
Eiichiro Sonoda ◽  
Akira Fujimori ◽  
Yukiko M. Yamashita ◽  
Shunichi Takeda
Keyword(s):  
Dna Repair ◽  
Experimental Evidence ◽  
Cell Line ◽  
Dt40 Cell ◽  
Unique Model ◽  
Chicken Dt40 Cell

scholarly journals The role of HERC2 and RNF8 ubiquitin E3 ligases in the promotion of translesion DNA synthesis in the chicken DT40 cell line

DNA Repair ◽  
2016 ◽  
Vol 40 ◽  
pp. 67-76 ◽  
Author(s):  
Mohiuddin ◽  
Shunsuke Kobayashi ◽  
Islam Shamima Keka ◽  
Guillaume Guilbaud ◽  
Julian Sale ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Cell Line ◽  
E3 Ligases ◽  
Translesion Dna Synthesis ◽  
Dt40 Cell ◽  
Translesion Dna ◽  
Chicken Dt40 Cell

Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line

2004 ◽  
Vol 271 (2) ◽  
pp. 398-404 ◽  
Author(s):  
Francois Foulquier ◽  
Sandrine Duvet ◽  
Andre Klein ◽  
Anne-Marie Mir ◽  
Frederic Chirat ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Line ◽  
Cho Cell ◽  
Endoplasmic Reticulum Associated Degradation ◽  
Cho Cell Line

scholarly journals Reduced X-Ray Resistance and Homologous Recombination Frequencies in a RAD54−/− Mutant of the Chicken DT40 Cell Line

Cell ◽  
1997 ◽  
Vol 89 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Olga Bezzubova ◽  
Arkadiy Silbergleit ◽  
Yuko Yamaguchi-Iwai ◽  
Shunichi Takeda ◽  
Jean-Marie Buerstedde
Keyword(s):  
Homologous Recombination ◽  
Cell Line ◽  
X Ray ◽  
Dt40 Cell ◽  
Chicken Dt40 Cell

A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics

Gene ◽  
2007 ◽  
Vol 395 (1-2) ◽  
pp. 116-124 ◽  
Author(s):  
Hiroshi Honda ◽  
Kenji Ichiyanagi ◽  
Jun Suzuki ◽  
Takao Ono ◽  
Hideki Koyama ◽  
...  
Keyword(s):  
Cell Line ◽  
Reverse Genetics ◽  
Dt40 Cell ◽  
Chicken Dt40 Cell ◽  
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