Tb3+-enhanced Potentiometric Detection of Single Nucleotide Polymorphism by Field Effect Transistors

2009 ◽  
Vol 38 (4) ◽  
pp. 376-377 ◽  
Author(s):  
Jinping Wang ◽  
Kenya Ito ◽  
Takuya Nakanishi ◽  
Shigeki Kuroiwa ◽  
Tetsuya Osaka
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Potentiometric Detection

scholarly journals Single Nucleotide Polymorphism Detection Using Au-Decorated Single-Walled Carbon Nanotube Field Effect Transistors

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Keum-Ju Lee ◽  
Hye-Mi So ◽  
Byoung-Kye Kim ◽  
Do Won Kim ◽  
Jee-Hwan Jang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Field Effect Transistors ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide Polymorphism Detection ◽  
Single Nucleotide ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Au Clusters ◽  
Polymorphism Detection ◽  
Au Cluster

We demonstrate that Au-cluster-decorated single-walled carbon nanotubes (SWNTs) may be used to discriminate single nucleotide polymorphism (SNP). Nanoscale Au clusters were formed on the side walls of carbon nanotubes in a transistor geometry using electrochemical deposition. The effect of Au cluster decoration appeared as hole doping when electrical transport characteristics were examined. Thiolated single-stranded probe peptide nucleic acid (PNA) was successfully immobilized on Au clusters decorating single-walled carbon nanotube field-effect transistors (SWNT-FETs), resulting in a conductance decrease that could be explained by a decrease in Au work function upon adsorption of thiolated PNA. Although a target single-stranded DNA (ssDNA) with a single mismatch did not cause any change in electrical conductance, a clear decrease in conductance was observed with matched ssDNA, thereby showing the possibility of SNP (single nucleotide polymorphism) detection using Au-cluster-decorated SWNT-FETs. However, a power to discriminate SNP target is lost in high ionic environment. We can conclude that observed SNP discrimination in low ionic environment is due to the hampered binding of SNP target on nanoscale surfaces in low ionic conditions.

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