Simultaneous On-chip Surface Plasmon Resonance Measurement of Disease Marker Protein and Small Metabolite Combined with Immuno- and Enzymatic Reactions

2008 ◽  
Vol 37 (7) ◽  
pp. 698-699 ◽  
Author(s):  
Kohei Nakamoto ◽  
Ryoji Kurita ◽  
Naoyuki Sekioka ◽  
Osamu Niwa
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Enzymatic Reactions ◽  
Marker Protein ◽  
Chip Surface ◽  
Resonance Measurement ◽  
Disease Marker ◽  
On Chip

On-Chip Surface Plasmon Resonance Sensor

2008 ◽  
Vol 8 (10) ◽  
pp. 4968-4971 ◽  
Author(s):  
Hyungseok Pang ◽  
HyoungJ. Cho ◽  
PatrickL. Likamwa
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Sensor ◽  
Chip Surface ◽  
Resonance Sensor ◽  
On Chip

A Surface Plasmon Resonance Based Inhibition Immunoassay for Sensitive and Selective Detection of 17β-Estradiol

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Quantitative Measurement ◽  
Dynamic Range ◽  
Indirect Detection ◽  
Negative Slope ◽  
Chip Surface ◽  
Detection Mechanism ◽  
17Β Estradiol

<div> <div> <div> <p>Quantitative measurement of small-molecule metabolites is now emerging as an effective way to link the metabolite profile to disease state. Surface plasmon resonance (SPR) is a sensing platform that has demonstrated applicability for a large range of biomolecules. However, direct detection of small molecules with SPR challenges the refractive index based detection mechanism. Herein, we utilized an indirect detection format and developed an inhibition immunoassay for the quantitative measurement of 17β-estradiol (E2) using SPR. One competitor, BSA-E2 conjugate, was immobilized to the SPR chip via the reaction between the primary amino group of the conjugate and the succinimide group (NHS) introduced by the formation of a thiol-NHS monolayer on gold surface. Free E2 molecules compete with BSA-E2 on chip surface for binding sites provided by a monoclonal anti-E2 antibody. It was found the binding affinity of the antibody to BSA-E2 conjugate increases with decreasing surface coverage of BSA-E2 conjugate. Under optimal conditions, a sigmoidal calibration curve with a negative slope and a dynamic range from 10 pM to 2 nM was generated. The detection limit of the immunoassay is estimated to be 0.3 pM. Moreover, the immunoassay exhibits high specificity for E2 detection using estrone (E1) as a potential interference.</p></div></div></div>

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

Patterned cellulose membrane for surface plasmon resonance measurement

2012 ◽  
Vol 173 ◽  
pp. 354-360 ◽  
Author(s):  
Toru Miura ◽  
Tsutomu Horiuchi ◽  
Yuzuru Iwasaki ◽  
Michiko Seyama ◽  
Serge Camou ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cellulose Membrane ◽  
Resonance Measurement
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