Self-assembly of Horseradish Peroxidase on Biocompatible Gold Nanoparticles–Vaterite Core–Shell Composite and its Direct Electrochemistry

2005 ◽  
Vol 34 (6) ◽  
pp. 832-833 ◽  
Author(s):  
Qin Xu ◽  
Wen-Yi Cai ◽  
Jun-Jie Zhu
Sensors ◽  
2009 ◽  
Vol 9 (2) ◽  
pp. 881-894 ◽  
Author(s):  
Gautham Kumar Ahirwal ◽  
Chanchal Mitra

2013 ◽  
Vol 104 ◽  
pp. 181-185 ◽  
Author(s):  
Qijin Wan ◽  
Hongtu Song ◽  
Hao Shu ◽  
Zhaohao Wang ◽  
Jing Zou ◽  
...  

2013 ◽  
Vol 704 ◽  
pp. 72-76
Author(s):  
Han Cui ◽  
Zhao Hao Wang ◽  
Qi Jin Wan ◽  
Nian Jun Yang

The H2O2 biosensor was prepared by continuous processes: 2,3-dimercaptosuccinic acid (DMSA) self-assembly monolayers gold nanoparticles (AuNPs) film by electrodepositioncovalent immobilization of horseradish peroxidase (HRP). In pH 7.2 phosphoric buffer solution (PBS), the HRP-AuNPs-DMSA-Au electrode exhibited a pair of well-defined cyclic voltammetric peaks in H2O2 solution, and the oxidation peak is about +0.4V versus saturated calomel electrode (SCE). The resulting substrates were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor exhibited remarkable sensitivity towards H2O2 and a wide dynamic range of 2-800 μM. The electrode retained 90% of its initial activity after 30 days of storage at 4°C.


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