An Artificial Signal Transduction System. Control of Lactate Dehydrogenase Activity Performed by an Artificial Cell-surface Receptor

1999 ◽  
Vol 28 (3) ◽  
pp. 253-254 ◽  
Author(s):  
Jun-ichi Kikuchi ◽  
Katsuhiko Ariga ◽  
Tatsuo Miyazaki ◽  
Kouki Ikeda
Keyword(s):  
Signal Transduction ◽  
Lactate Dehydrogenase ◽  
Cell Surface ◽  
Dehydrogenase Activity ◽  
Cell Surface Receptor ◽  
System Control ◽  
Signal Transduction System ◽  
Lactate Dehydrogenase Activity ◽  
Artificial Cell ◽  
Surface Receptor

scholarly journals Brassinosteroid signal transduction from cell-surface receptor kinases to nuclear transcription factors

2009 ◽  
Vol 11 (10) ◽  
pp. 1254-1260 ◽  
Author(s):  
Tae-Wuk Kim ◽  
Shenheng Guan ◽  
Yu Sun ◽  
Zhiping Deng ◽  
Wenqiang Tang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Transcription Factors ◽  
Cell Surface ◽  
Cell Surface Receptor ◽  
Receptor Kinases ◽  
Surface Receptor

scholarly journals Constitutive Activation and Inactivation of Mutations Inducing Cell Surface Loss of Receptor and Impairing of Signal Transduction of Agonist-Stimulated Eel Follicle-Stimulating Hormone Receptor

2020 ◽  
Vol 21 (19) ◽  
pp. 7075
Author(s):  
Munkhzaya Byambaragchaa ◽  
Jeong-Soo Kim ◽  
Hong-Kyu Park ◽  
Dae-Jung Kim ◽  
Sun-Mee Hong ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cell Surface ◽  
Hormone Receptor ◽  
Follicle Stimulating Hormone ◽  
Chinese Hamster ◽  
Cell Surface Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wild Type ◽  
Surface Receptor ◽  
Stimulating Hormone

In the present study, we investigated the signal transduction of mutants of the eel follicle-stimulating hormone receptor (eelFSHR). Specifically, we examined the constitutively activating mutant D540G in the third intracellular loop, and four inactivating mutants (A193V, N195I, R546C, and A548V). To directly assess functional effects, we conducted site-directed mutagenesis to generate mutant receptors. We measured cyclic adenosine monophosphate (cAMP) accumulation via homogeneous time-resolved fluorescence assays in Chinese hamster ovary (CHO-K1) cells and investigated cell surface receptor loss using an enzyme-linked immunosorbent assay in human embryonic kidney (HEK) 293 cells. The cells expressing eelFSHR-D540G exhibited a 23-fold increase in the basal cAMP response without agonist treatment. The cells expressing A193V, N195I, and A548V mutants had completely impaired signal transduction, whereas those expressing the R546C mutant exhibited little increase in cAMP responsiveness and a small increase in signal transduction. Cell surface receptor loss in the cells expressing inactivating mutants A193V, R546C, and A548V was clearly slower than in the cell expressing the wild-type eelFSHR. However, cell surface receptor loss in the cells expressing inactivating mutant N195I decreased in a similar manner to that of the cells expressing the wild-type eelFSHR or the activating mutant D540G, despite the completely impaired cAMP response. These results provide important information regarding the structure–function relationships of G protein-coupled receptors during signal transduction.

Dynamic behavior of a transmembrane molecular switch as an artificial cell-surface receptor

2001 ◽  
Vol 11 (4-6) ◽  
pp. 971-976 ◽  
Author(s):  
Kentaro Fukuda ◽  
Yoshihiro Sasaki ◽  
Katsuhiko Ariga ◽  
Jun-ichi Kikuchi
Keyword(s):  
Cell Surface ◽  
Dynamic Behavior ◽  
Molecular Switch ◽  
Cell Surface Receptor ◽  
Artificial Cell ◽  
Surface Receptor
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