Oxidoreduction between Cycloalkanols and Cycloalkanones in the Cultured Cells ofNicotiana tabacum. Simulation of the Time-courses in the Oxidation of (+)-Borneol and the Reduction of (−)-Carvomenthone

1987 ◽  
Vol 16 (2) ◽  
pp. 425-428 ◽  
Author(s):  
Takayuki Suga ◽  
Shunsuke Izumi ◽  
Toshifumi Hirata ◽  
Hiroki Hamada
Keyword(s):  
Cultured Cells ◽  
Ofnicotiana Tabacum ◽  
Time Courses

scholarly journals Inward fluxes of adenosine in erythrocytes and cultured cells measured by a quenched-flow method

1984 ◽  
Vol 224 (3) ◽  
pp. 1001-1008 ◽  
Author(s):  
A R P Paterson ◽  
E R Harley ◽  
C E Cass
Keyword(s):  
Cellular Uptake ◽  
Cultured Cells ◽  
Flow System ◽  
Kinetic Constants ◽  
Cell Water ◽  
Short Intervals ◽  
Adenosine Uptake ◽  
Uptake Rates ◽  
Time Courses ◽  
Initial Uptake

Dilazep, a vasodilator previously recognized as an inhibitor of adenosine permeation, very rapidly blocked the uptake of adenosine by cultured L5178Y cells, and accordingly was used as a quencher in a simple quenched-flow system for measuring cellular uptake of nucleosides during very short intervals. Time courses of cellular uptake of adenosine, assayed during intervals between 0.05 and 0.5s with the quenched-flow system, were linear and defined initial rates of adenosine uptake. The latter are rates of inward transport of adenosine. Kinetic constants for that process in cultured S49 cells determined with the quenched-flow procedure were similar to those determined with an assay dependent on manual timing. In studies of adenosine uptake kinetics in human erythrocytes at 22 degrees C and 37 degrees C in which the quenched-flow procedure was used, time courses of adenosine uptake were linear at both temperatures and defined initial uptake rates; kinetic constants (means +/- S.E.M.) at 22 degrees C (n = 8) were Km 25 +/- 14 microM and Vmax. 15 +/- 5 pmol/s per microliter of cell water and at 37 degrees C (n = 3) were Km 98 +/- 17 microM and Vmax. 80 +/- 9 pmol/s per microliter of cell water.

Asymmetric epoxidation of digeranyl by cultured cells ofNicotiana tabacum

2003 ◽  
Vol 46 (5) ◽  
pp. 401-409 ◽  
Author(s):  
Osamu Nakagawa ◽  
Kei Shimoda ◽  
Sunsuke Izumi ◽  
Toshifumi Hirata
Keyword(s):  
Cultured Cells ◽  
Asymmetric Epoxidation ◽  
Ofnicotiana Tabacum

Brassinosteroids and Monoglycerides with Brassinosteroid-like Activity in Immature Seeds ofOryza sativaandPerilla frutescensand in Cultured Cells ofNicotiana tabacum

1994 ◽  
Vol 58 (12) ◽  
pp. 2241-2243 ◽  
Author(s):  
Keun-Hyung Park ◽  
Jong-Dae Park ◽  
Kyu-Hawn Hyun ◽  
Masayoshi Nakayama ◽  
Takao Yokota
Keyword(s):  
Cultured Cells ◽  
Ofnicotiana Tabacum ◽  
Immature Seeds

scholarly journals Interaction between DNA-damage protein GADD34 and a new member of the Hsp40 family of heat shock proteins that is induced by a DNA-damaging reagent

2000 ◽  
Vol 352 (3) ◽  
pp. 795-800 ◽  
Author(s):  
Tadao HASEGAWA ◽  
Hengyi XIAO ◽  
Fumiyasu HAMAJIMA ◽  
Ken-ichi ISOBE
Keyword(s):  
Dna Damage ◽  
Heat Shock ◽  
Cultured Cells ◽  
Similar Region ◽  
C Terminus ◽  
Mouse Tissues ◽  
Time Courses ◽  
Shock Proteins ◽  
Two Hybrid

GADD34 is one of a subset of proteins induced after DNA damage or cell growth arrest. To examine the function of GADD34, we used the yeast two-hybrid system to clone the protein that interacts with murine GADD34. As bait we used the product of the partial GADD34 cDNA, including the regions rich in proline, glutamic acid, serine and threonine (PEST) and γ134.5 regions. A cDNA clone, named GAHSP40, which is a mouse DnaJ family protein with a high similarity to human HLJ1 was cloned. The interaction between GADD34 and GAHSP40 in cultured cells was confirmed by a co-immunoprecipitation experiment and in NIH 3T3 cells by two-hybrid analysis in vivo. For binding of the two proteins, the γ134.5-similar region of GADD34 was necessary; however, the PEST region was also involved and the C-terminus of GAHSP40, but not the J-domain, was important. GAHSP40 was detected in all mouse tissues examined, but a different transcript was found in the testis. Both GADD34 mRNA and GAHSP40 mRNA were significantly elevated by treatment with methyl methanesulphonate, although the time courses were different. In addition, both GAHSP40 and GADD34 mRNA were induced by heat shock.

Cloning of cDNA Encoding Ethylene-Responsive Element Binding Protein-5 in the Cultured Cells ofNicotiana tabacum

DNA Sequence ◽  
2000 ◽  
Vol 11 (1-2) ◽  
pp. 125-129 ◽  
Author(s):  
Yoshiyuki Ashida ◽  
Naoyuki Yokobatake ◽  
Chie Kohchi ◽  
Kei Shimoda ◽  
Toshifumi Hirata
Keyword(s):  
Cultured Cells ◽  
Binding Protein ◽  
Ofnicotiana Tabacum ◽  
Element Binding Protein ◽  
Responsive Element

scholarly journals Stereoselectivity in Oxidative and Reductive Transformations ofp-Menthane Derivatives with the Cultured Cells ofNicotiana tabacum

1987 ◽  
Vol 16 (5) ◽  
pp. 903-906 ◽  
Author(s):  
Takayuki Suga ◽  
Hiroki Hamada ◽  
Toshifumi Hirata ◽  
Shunsuke Izumi
Keyword(s):  
Cultured Cells ◽  
Ofnicotiana Tabacum

THE ENANTIOSELECTIVE BIOTRANSFORMATION OF α-TERPINEOL AND ITS ACETATE WITH THE CULTURED CELLS OFNICOTIANA TABACUM

1982 ◽  
Vol 11 (10) ◽  
pp. 1595-1598 ◽  
Author(s):  
Takayuki Suga ◽  
Toshifumi Hirata ◽  
Ym Sook Lee
Keyword(s):  
Cultured Cells ◽  
Ofnicotiana Tabacum
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