Site-specific Manipulation of Mitochondrial DNA by Artificial Restriction DNA Cutter

2019 ◽  
Vol 48 (11) ◽  
pp. 1332-1335
Author(s):  
Toshimasa Harumoto ◽  
Narumi Shigi ◽  
Kouhei Tsumoto ◽  
Makoto Komiyama
Keyword(s):  
Mitochondrial Dna ◽  
Site Specific ◽  
Artificial Restriction

scholarly journals Maximum-Likelihood Estimation of Site-Specific Mutation Rates in Human Mitochondrial DNA From Partial Phylogenetic Classification

Genetics ◽  
2008 ◽  
Vol 180 (3) ◽  
pp. 1511-1524 ◽  
Author(s):  
Saharon Rosset ◽  
R. Spencer Wells ◽  
David F. Soria-Hernanz ◽  
Chris Tyler-Smith ◽  
Ajay K. Royyuru ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Maximum Likelihood ◽  
Maximum Likelihood Estimation ◽  
Likelihood Estimation ◽  
Mutation Rates ◽  
Site Specific ◽  
Human Mitochondrial Dna ◽  
Specific Mutation ◽  
Phylogenetic Classification

scholarly journals Site-specific somatic mitochondrial DNA point mutations in patients with thymidine phosphorylase deficiency

2003 ◽  
Vol 111 (12) ◽  
pp. 1913-1921 ◽  
Author(s):  
Yutaka Nishigaki ◽  
Ramon Martí ◽  
William C. Copeland ◽  
Michio Hirano
Keyword(s):  
Mitochondrial Dna ◽  
Thymidine Phosphorylase ◽  
Point Mutations ◽  
Site Specific

Site-specific Scission of Lambda Phage Genomic DNA by Ce(IV)/EDTA-based Artificial Restriction DNA Cutter

2006 ◽  
Vol 35 (6) ◽  
pp. 594-595 ◽  
Author(s):  
Yoji Yamamoto ◽  
Kazuyuki Miura ◽  
Makoto Komiyama
Keyword(s):  
Genomic Dna ◽  
Lambda Phage ◽  
Site Specific ◽  
Artificial Restriction

A site-specific DNA endonuclease specified by one of two ORFs encoded by a group I intron in Dictyostelium discoideum mitochondrial DNA

Gene ◽  
1997 ◽  
Vol 191 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Shinji Ogawa ◽  
Kayo Naito ◽  
Kiyohiko Angata ◽  
Takahiro Morio ◽  
Hideko Urushihara ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dictyostelium Discoideum ◽  
Group I Intron ◽  
Dna Endonuclease ◽  
Site Specific ◽  
Group I ◽  
A Site

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

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