scholarly journals Effects of Molecular-crowding on Electron Transfer and Oxidative Damage in Pyrene-modified Oligonucleotides

2018 ◽  
Vol 47 (1) ◽  
pp. 62-64 ◽  
Author(s):  
Makiko Tanaka ◽  
Hiroki Iida ◽  
Takayuki Matsumoto
Keyword(s):  
Electron Transfer ◽  
Oxidative Damage ◽  
Molecular Crowding ◽  
Modified Oligonucleotides

scholarly journals NADPH Inhibits [2Fe-2S] Cluster Protein Transfer from Diabetes Drug Target MitoNEET to an Apo-acceptor Protein

2012 ◽  
Vol 287 (15) ◽  
pp. 11649-11655 ◽  
Author(s):  
John A. Zuris ◽  
Syed S. Ali ◽  
Howard Yeh ◽  
Tung A. Nguyen ◽  
Rachel Nechushtai ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Oxidative Damage ◽  
Drug Target ◽  
Protein Transfer ◽  
Physiological Conditions ◽  
Founding Member ◽  
Cluster Transfer ◽  
Dependent Inhibition ◽  
Diabetes Drug ◽  
Transfer Properties

MitoNEET (mNT) is the founding member of the recently discovered CDGSH family of [2Fe-2S] proteins capable of [2Fe-2S] cluster transfer to apo-acceptor proteins. It is a target of the thiazolidinedione (TZD) class of anti-diabetes drugs whose binding modulate both electron transfer and cluster transfer properties. The [2Fe-2S] cluster in mNT is destabilized upon binding of NADPH, which leads to loss of the [2Fe-2S] cluster to the solution environment. Because mNT is capable of transferring [2Fe-2S] clusters to apo-acceptor proteins, we sought to determine whether NADPH binding also affects cluster transfer. We show that NADPH inhibits transfer of the [2Fe-2S] cluster to an apo-acceptor protein with an inhibition constant (Ki) of 200 μm, which reflects that of NADPH concentrations expected under physiological conditions. In addition, we determined that the strictly conserved cluster interacting residue Asp-84 in the CDGSH domain is necessary for the NADPH-dependent inhibition of [2Fe-2S] cluster transfer. The most critical cellular function of NADPH is in the maintenance of a pool of reducing equivalents, which is essential to counteract oxidative damage. Taken together, our findings suggest that NADPH can regulate both mNT [2Fe-2S] cluster levels in the cell as well as the ability of the protein to transfer [2Fe-2S] clusters to cytosolic or mitochondrial acceptors.

scholarly journals Mechanism of protein oxidative damage that is coupled to long-range electron transfer to high-valent haems

2016 ◽  
Vol 473 (12) ◽  
pp. 1769-1775 ◽  
Author(s):  
Zhongxin Ma ◽  
Heather R. Williamson ◽  
Victor L. Davidson
Keyword(s):  
Reactive Oxygen Species ◽  
Electron Transfer ◽  
Oxidative Damage ◽  
Long Range ◽  
Direct Contact ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
High Valent

The present study describes how oxidative damage to a protein may occur without direct contact with a reactive oxygen species, and how that radical-mediated damage can be propagated through the protein. This process is coupled to the reactivity of high-valent haems within the same protein.

scholarly journals Molecular crowding effects on the biochemical properties of amyloid β–heme, Aβ–Cu and Aβ–heme–Cu complexes

2020 ◽  
Vol 11 (28) ◽  
pp. 7479-7486 ◽  
Author(s):  
Meng Li ◽  
Zhenqi Liu ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Oxidative Damage ◽  
Amyloid Β ◽  
Biochemical Properties ◽  
Molecular Crowding ◽  
Crowding Effects ◽  
Cu Complexes

Our findings highlight the significance of hydration contributions to the interactions of Aβ–heme and Aβ–Cu as well as their peroxidase activities. Aβ40–Cu but not Aβ40–heme may play more important roles in the oxidative damage in the etiology of AD.

Attenuation of guanine oxidation via DNA-mediated electron transfer in a crowded environment using small cosolutes

2018 ◽  
Vol 16 (36) ◽  
pp. 6695-6702 ◽  
Author(s):  
Makiko Tanaka ◽  
Takayuki Matsumoto ◽  
Hiroki Iida
Keyword(s):  
Electron Transfer ◽  
Molecular Crowding ◽  
Guanine Oxidation ◽  
Mediated Electron Transfer ◽  
Modified Oligonucleotide ◽  
Crowded Environment

Guanine oxidation induced by photoirradiation on a pyrene-modified oligonucleotide was investigated under molecular crowding using small cosolutes such as glycerol.

Long range electron transfer (LRET) in proteins: Implications for oxidative damage

1990 ◽  
Vol 9 ◽  
pp. 58
Author(s):  
Michael H. Klapper ◽  
Michael R. DeFelippis ◽  
Hyosil Lee ◽  
M. Faraggi
Keyword(s):  
Electron Transfer ◽  
Oxidative Damage ◽  
Long Range

The role of molecular crowding in long-range metalloprotein electron transfer: Dissection into site- and scaffold-specific contributions

2019 ◽  
Vol 294 ◽  
pp. 117-125 ◽  
Author(s):  
Ulises A. Zitare ◽  
Jonathan Szuster ◽  
Magali F. Scocozza ◽  
Andrés Espinoza-Cara ◽  
Alcides J. Leguto ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Long Range ◽  
Molecular Crowding

scholarly journals DNA-Mediated Electron Transfer in Naphthalene-Modified Oligonucleotides

2010 ◽  
Vol 75 (8) ◽  
pp. 2423-2428 ◽  
Author(s):  
Makiko Tanaka ◽  
Benjamin Elias ◽  
Jacqueline K. Barton
Keyword(s):  
Electron Transfer ◽  
Modified Oligonucleotides ◽  
Mediated Electron Transfer

LONG RANGE ELECTRON TRANSFER (LRET) IN PROTEINS: IMPLICATIONS FOR OXIDATIVE DAMAGE

1991 ◽  
pp. 246-250 ◽  
Author(s):  
Hyosil Lee ◽  
Michael H. DeFelippis ◽  
M. Faraggi ◽  
Michael H. Klapper
Keyword(s):  
Electron Transfer ◽  
Oxidative Damage ◽  
Long Range
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