Electroanalytical Application of Screen-printed Carbon Electrode Modified with Conductive Graphene Oxide–Poly(acrylic acid) Film for Label-free Detection of Human Immunoglobulin G

2016 ◽  
Vol 45 (12) ◽  
pp. 1444-1446
Author(s):  
Poachanee Norfun ◽  
Watthanachai Jumpathong ◽  
Nawee Kungwan ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Graphene Oxide ◽  
Acrylic Acid ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Carbon Electrode ◽  
Label Free ◽  
Human Immunoglobulin G ◽  
Screen Printed Carbon Electrode ◽  
Label Free Detection ◽  
Screen Printed

scholarly journals A Redox Cu(II)-Graphene Oxide Modified Screen Printed Carbon Electrode as a Cost-Effective and Versatile Sensing Platform for Electrochemical Label-Free Immunosensor and Non-enzymatic Glucose Sensor

2021 ◽  
Vol 9 ◽  
Author(s):  
Sopit Phetsang ◽  
Duangruedee Khwannimit ◽  
Parawee Rattanakit ◽  
Narong Chanlek ◽  
Pinit Kidkhunthod ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrochemical Sensors ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Carbon Electrode ◽  
Label Free ◽  
Enzymatic Sensor ◽  
Screen Printed Carbon Electrode ◽  
Electrochemical Label ◽  
Screen Printed

A novel copper (II) ions [Cu(II)]-graphene oxide (GO) nanocomplex-modified screen-printed carbon electrode (SPCE) is successfully developed as a versatile electrochemical platform for construction of sensors without an additionally external redox probe. A simple strategy to prepare the redox GO-modified SPCE is described. Such redox GO based on adsorbed Cu(II) is prepared by incubation of GO-modified SPCE in the Cu(II) solution. This work demonstrates the fabrications of two kinds of electrochemical sensors, i.e., a new label-free electrochemical immunosensor and non-enzymatic sensor for detections of immunoglobulin G (IgG) and glucose, respectively. Our immunosensor based on square-wave voltammetry (SWV) of the redox GO-modified electrode shows the linearity in a dynamic range of 1.0–500 pg.mL−1 with a limit of detection (LOD) of 0.20 pg.mL−1 for the detection of IgG while non-enzymatic sensor reveals two dynamic ranges of 0.10–1.00 mM (sensitivity = 36.31 μA.mM−1.cm−2) and 1.00–12.50 mM (sensitivity = 3.85 μA.mM−1.cm−2) with a LOD value of 0.12 mM. The novel redox Cu(II)-GO composite electrode is a promising candidate for clinical research and diagnosis.

scholarly journals Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman
Keyword(s):  
Graphene Oxide ◽  
Limit Of Detection ◽  
Food Product ◽  
Electrochemical Immunosensor ◽  
Growth Promoter ◽  
Carbon Electrode ◽  
Electrochemical Assay ◽  
Sensor Platform ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

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