Module Strategy for Peptide Ribonucleic Acid (PRNA)–DNA and PRNA–Peptide Nucleic Acid (PNA)–DNA Chimeras: Synthesis and Interaction of Chimeras with DNA and RNA

2016 ◽  
Vol 45 (3) ◽  
pp. 350-352 ◽  
Author(s):  
Ryohei Uematsu ◽  
Masahito Inagaki ◽  
Mitsuo Asai ◽  
Hiroka Sugai ◽  
Yoshiki Maeda ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Ribonucleic Acid ◽  
Peptide Nucleic Acid ◽  
Dna And Rna

Synthesis of α-(R)-/γ-(S)-Dimethyl Substituted Peptide Nucleic Acid Submonomer Using Mitsunobu Reaction

2019 ◽  
Vol 16 (5) ◽  
pp. 437-446
Author(s):  
Ahmed S. Abdelbaky ◽  
Ivan A. Prokhorov ◽  
Igor P. Smirnov ◽  
Kristina M. Koroleva ◽  
Vitaliy I. Shvets ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Solid Phase ◽  
Genetic Diseases ◽  
Mitsunobu Reaction ◽  
Phase Synthesis ◽  
Dna And Rna ◽  
Pna Synthesis ◽  
Biomedical Technologies

One of the major challenges facing modern biochemical and biomedical technologies are finding molecular tools for diagnosis and detection of genetic diseases. In this connection, several classes of oligonucleotides have been developed that can recognize and bind to DNA and RNA with high affinity and sequence selectivity and withstand enzymatic degradation by proteases and nucleases; however, few can traverse the cell membrane on their own. One such promising class of nucleic acid mimics developed in the last two decades which showed good results in vitro, are the peptide nucleic acids (PNAs). New chiral α- and γ-peptide Nucleic Acid (PNA) submonomer with methyl substituents in pseudopeptide backbone were synthesized via Mitsunobu reaction. The α-(R)-/γ-(S)-configuration of the chiral centres will ensure the preorganization of the PNA oligomer into a right-handed helix. The results obtained showed that Boc/Fmoc-submonomer compatible with Boc-protocol PNAs solid-phase synthesis on an MBHA resin. We synthesized simple and efficient α-R-, γ-S-disubstituted PNA submonomer based on L-Ala and D-Ala with the construction of the intermediate pseudopeptide moiety by Mitsunobu reaction for subsequent use in the Boc-Protocol of solid phase PNA synthesis.

EFFECT OF OESTRADIOL-17β ON CLEAVAGE, NUCLEIC ACID METABOLISM AND PROTEIN SYNTHESIS IN SEA URCHIN, STRONGYLOCENTROTUS PURPURATUS, EMBRYOS

1962 ◽  
Vol 25 (2) ◽  
pp. 183-NP ◽  
Author(s):  
W. B. JOLLEY ◽  
W. E. MARTIN ◽  
J. W. BAMBERGER ◽  
L. W. STEARNS
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Acid Metabolism ◽  
Strongylocentrotus Purpuratus ◽  
Nucleic Acid Metabolism ◽  
Dna And Rna ◽  
Moderate Effect

SUMMARY Oestradiol-17β at a concentration of 3 × 10−3 m inhibits cleavage in sea urchin (Strongylocentrotus purpuratus) embryos. This inhibition is accompanied by a reduction in deoxyribonucleic acid (DNA) synthesis and little change in ribonucleic acid (RNA). The effects of oestradiol-17β upon the incorporation of glycine-1-14C and glycine-2-14C into the purines of DNA and RNA and the incorporation of glycine-2-14C into serine were studied. The incorporation of glycine-1-14C and glycine-2-14C into RNA was reduced, but the incorporation of glycine-2-14C into DNA was increased considerably over that of the controls. The incorporation of glycine-2-14C into serine was also accelerated by oestradiol. A possible explanation of the action of oestradiol-17β is offered. The moderate effect upon RNA is not surprising because there is little or no synthesis of this compound from the time of fertilization to blastulation under normal conditions.

A Cyclopentane Conformational Restraint for a Peptide Nucleic Acid:  Design, Asymmetric Synthesis, and Improved Binding Affinity to DNA and RNA

2003 ◽  
Vol 5 (15) ◽  
pp. 2695-2698 ◽  
Author(s):  
Michael C. Myers ◽  
Mark A. Witschi ◽  
Nataliya V. Larionova ◽  
John M. Franck ◽  
Russell D. Haynes ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Asymmetric Synthesis ◽  
Binding Affinity ◽  
Peptide Nucleic Acid ◽  
Dna And Rna

DNA and RNA binding properties of an arginine-based ‘Extended Chiral Box’ Peptide Nucleic Acid

2011 ◽  
Vol 52 (2) ◽  
pp. 300-304 ◽  
Author(s):  
Alessandro Calabretta ◽  
Tullia Tedeschi ◽  
Roberto Corradini ◽  
Rosangela Marchelli ◽  
Stefano Sforza
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Rna Binding ◽  
Binding Properties ◽  
Dna And Rna ◽  
Dna And Rna Binding
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