Cooperative Ligand Binding to Globular Protein(II). Binding of Dianionic Azo Dyes to Hen Egg White Lysozyme

1999 ◽  
Vol 72 (4) ◽  
pp. 653-659 ◽  
Author(s):  
Kiyofumi Murakami ◽  
Katsuyoshi Tsurufuji
Keyword(s):  
Ligand Binding ◽  
Azo Dyes ◽  
Egg White ◽  
Globular Protein ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

The Chitopentaose Complex of a Mutant Hen Egg-White Lysozyme Displays No Distortion of the –1 Sugar Away from a 4C1 Chair Conformation

2009 ◽  
Vol 62 (6) ◽  
pp. 528 ◽  
Author(s):  
Gideon J. Davies ◽  
Stephen G. Withers ◽  
David J. Vocadlo
Keyword(s):  
Ligand Binding ◽  
Transition State ◽  
Chair Conformation ◽  
Egg White ◽  
Glycosidase Inhibitors ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Oxocarbenium Ion ◽  
Hen Egg White ◽  
Hen Egg

Glycosidase inhibitors frequently reflect either the charge or the ‘flattened’ shape of the oxocarbenium-ion like transition state. Much of the impetus for such inhibitory strategies derives from historical studies on ligand binding to hen egg white lysozyme (HEWL); not least those suggesting that product complexes of the enzyme showed distortion of the pyranosides in the –1 subsite. Ironically, while distortion is undoubtedly a defining feature of glycosidases, product complexes themselves are rarely distorted. Here we show that the chitopentaose product complex of a mutant E35Q HEWL, solved at 1.8 Å resolution, is bound with all sugars in 4C1 conformation.

The interaction of anionic azo dyes with hen egg-white lysozyme: 2

1982 ◽  
Vol 260 (3) ◽  
pp. 339-344 ◽  
Author(s):  
Y. Nakano ◽  
S. Kawauchi ◽  
J. Komiyama ◽  
T. Iijima
Keyword(s):  
Azo Dyes ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

The interaction of anionic azo dyes with hen egg-white lysozyme: 1

1982 ◽  
Vol 260 (2) ◽  
pp. 218-225 ◽  
Author(s):  
Y. Nakano ◽  
T. Seki ◽  
H. Iwata ◽  
J. Komiyama ◽  
T. Iijima
Keyword(s):  
Azo Dyes ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

Inactivation of hen egg-white lysozyme. The azide radical

1997 ◽  
Vol 94 ◽  
pp. 356-364 ◽  
Author(s):  
M Faraggi ◽  
E Bettelheim ◽  
M Weinstein
Keyword(s):  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

The inhibitory role of clioquinol in the fibrillation of hen egg white lysozyme

2021 ◽  
pp. 138830
Author(s):  
Baoliang Ma ◽  
Haohao Wang ◽  
Yujie Liu ◽  
Fang Wu ◽  
Xudong Zhu
Keyword(s):  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Inhibitory Role ◽  
Hen Egg White ◽  
Hen Egg
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