Effects of Trehalose on the Water Structure in Yeast Cells as Studied byin vivo1H NMR Spectroscopy

1995 ◽  
Vol 68 (12) ◽  
pp. 3621-3627 ◽  
Author(s):  
Minoru Sakurai ◽  
Hidetoshi Kawai ◽  
Yoshio Inoue ◽  
Akihiro Hino ◽  
Syouichi Kobayashi
Keyword(s):  
Nmr Spectroscopy ◽  
Water Structure ◽  
Yeast Cells

NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

2015 ◽  
Vol 11 (2) ◽  
pp. 379-383 ◽  
Author(s):  
C. Airoldi ◽  
F. Tripodi ◽  
C. Guzzi ◽  
R. Nicastro ◽  
P. Coccetti
Keyword(s):  
Nmr Spectroscopy ◽  
Sample Preparation ◽  
Rapid Method ◽  
Budding Yeast ◽  
Yeast Cells ◽  
Intracellular Metabolite ◽  
Nmr Analysis ◽  
H Nmr ◽  
Metabolite Extraction

We present a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells analyzed by1H-NMR spectroscopy.

Water Structure and Dynamics in Smectites: X-ray Diffraction and 2H NMR Spectroscopy of Mg–, Ca–, Sr–, Na–, Cs–, and Pb–Hectorite

2016 ◽  
Vol 120 (16) ◽  
pp. 8863-8876 ◽  
Author(s):  
U. Venkateswara Reddy ◽  
Geoffrey M. Bowers ◽  
Narasimhan Loganathan ◽  
Mark Bowden ◽  
A. Ozgur Yazaydin ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Water Structure ◽  
2H Nmr ◽  
X Ray Diffraction ◽  
X Ray ◽  
2H Nmr Spectroscopy ◽  
Structure And Dynamics

scholarly journals Water structure in vitro and within Saccharomyces cerevisiae yeast cells under conditions of heat shock

2008 ◽  
Vol 1780 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Jennifer L. Dashnau ◽  
Laura K. Conlin ◽  
Hillary C.M. Nelson ◽  
Jane M. Vanderkooi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Shock ◽  
Water Structure ◽  
Yeast Cells

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

Ultrathin frozen sections of yeast without aldehyde prefixation

1978 ◽  
Vol 36 (2) ◽  
pp. 58-59
Author(s):  
K. J. Böhm ◽  
a. E. Unger
Keyword(s):  
Aqueous Solutions ◽  
Biological Material ◽  
Yeast Cells ◽  
Frozen Sections ◽  
Good Preservation ◽  
Ultrathin Frozen Sections

During the last years it was shown that also by means of cryo-ultra-microtomy a good preservation of substructural details of biological material was possible. However the specimen generally was prefixed in these cases with aldehydes.Preparing ultrathin frozen sections of chemically non-prefixed material commonly was linked up to considerable technical and manual expense and the results were not always satisfying. Furthermore, it seems to be impossible to carry out cytochemical investigations by means of treating sections of unfixed biological material with aqueous solutions.We therefore tried to overcome these difficulties by preparing yeast cells (S. cerevisiae) in the following manner:

Deformation of Yeast Plasma Membrane by Ethidium Bromide

1988 ◽  
Vol 46 ◽  
pp. 254-255
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Thin Section ◽  
Ethidium Bromide ◽  
Freeze Fracture ◽  
Mutagenic Effect ◽  
Yeast Cells ◽  
Hydrophobic Region ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

Three-Dimensional Immunoelectron Microscopy of Yeast Cells by a New High-Resolution Replica Method

1985 ◽  
Vol 43 ◽  
pp. 562-563
Author(s):  
Hirano T. ◽  
M. Yamaguchi ◽  
M. Hayashi ◽  
Y. Sekiguchi ◽  
A. Tanaka
Keyword(s):  
High Resolution ◽  
Plasma Polymerization ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Replica Method ◽  
Yeast Cells ◽  
Dynamic Aspect ◽  
Replica Technique ◽  
Gaseous Hydrocarbon ◽  
Transmission Electron

A plasma polymerization film replica method is a new high resolution replica technique devised by Tanaka et al. in 1978. It has been developed for investigation of the three dimensional ultrastructure in biological or nonbiological specimens with the transmission electron microscope. This method is based on direct observation of the single-stage replica film, which was obtained by directly coating on the specimen surface. A plasma polymerization film was deposited by gaseous hydrocarbon monomer in a glow discharge.The present study further developed the freeze fracture method by means of a plasma polymerization film produces a three dimensional replica of chemically untreated cells and provides a clear evidence of fine structure of the yeast plasma membrane, especially the dynamic aspect of the structure of invagination (Figure 1).

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