scholarly journals Equilibrium and Kinetic Studies of the Interaction between Orange I and Bovine Serum Albumin

1988 ◽  
Vol 61 (4) ◽  
pp. 1323-1329 ◽  
Author(s):  
Kiyofumi Murakami
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Kinetic Studies

Pressure Jump Studies of Proteins. I. Isomerization of Bovine Serum Albumin at Neutral pH

1971 ◽  
Vol 49 (12) ◽  
pp. 1267-1275 ◽  
Author(s):  
D. E. Goldsack ◽  
P. M. Waern
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Conformational Change ◽  
Bovine Serum ◽  
Ph Dependence ◽  
Kinetic Studies ◽  
Relaxation Effect ◽  
Pressure Jump ◽  
Relaxation Effects ◽  
Ph Range

Pressure jump kinetic studies of the conformational change occurring in bovine serum albumin in neutral solutions have been carried out over the pH range 6.5–9.5. Two distinct relaxation effects are observed at each pH. The faster relaxation is attributed to binding of the dye to the protein, and the slower relaxation is related to the conformational change occurring in the protein. This slower relaxation effect is pH dependent with a maximum value near pH 8. Detailed analysis of these data leads to a mechanism for the conformational change which indicates that the one form of the protein has an ionizable group with a pK of 8.7 which changes to a pK of 6.7 when the protein undergoes the conformational change. A simple iterative procedure is given for analyzing the pH dependence of a relaxation time constant for a cyclic mechanism involving only one ionizing group controlling the conformational change.

Kinetic studies on binding of bovine serum albumin with 1-anilino-8-naphthalene sulfonate

FEBS Letters ◽  
1974 ◽  
Vol 43 (3) ◽  
pp. 293-296 ◽  
Author(s):  
Hiroshi Nakatani ◽  
Masafumi Haga ◽  
Keitaro Hiromi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Kinetic Studies

scholarly journals Stopped-flow Kinetic Studies of Cu2+Ion Binding to Bovine Serum Albumin

1984 ◽  
Vol 57 (10) ◽  
pp. 2712-2717 ◽  
Author(s):  
Naohisa Kure ◽  
Hitoshi Nakatsuji ◽  
Takayuki Sano ◽  
Tatsuya Yasunaga
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Kinetic Studies ◽  
Ion Binding ◽  
Stopped Flow
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