Introduction:
A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC)
method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances
Materials and Methods:
Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction
with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow
at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno,
Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute,
4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of
2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were
between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to
101.23±1.39% for Paracetamol.
Results and Discussion:
The validated optimal protocol is robust and accurate for simultaneous analysis
of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as
pharmaceutical formulation.
Conclusion:
In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection
was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical
preparations.
The Determination of a Small Amount of Biological Constituent by the Use of Chemiluminescence. IV. A Highly Sensitive Detector for the HPLC of Protein