scholarly journals Chromatographic Behavior of Metalloporphyrins by Cellulose Thin-layer Chromatography

1981 ◽  
Vol 54 (7) ◽  
pp. 1972-1974 ◽  
Author(s):  
Yoshiaki Onoue
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Behavior ◽  
Layer Chromatography

scholarly journals Reversed-phase thin-layer chromatography behavior of aldopentose derivatives

2012 ◽  
Vol 66 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Dragana Livaja-Popovic ◽  
Eva Loncar ◽  
Lidija Jevric ◽  
Radomir Malbasa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Chromatographic Behavior ◽  
Log P ◽  
Retention Factors ◽  
Mobile Phases ◽  
Acetone Water ◽  
Layer Chromatography

Quantitative structure-retention relationships (QSRR) have been used to study the chromatographic behavior of some aldopentose. The behavior of aldopentose derivatives was investigated by means of the reversed-phase thin-layer chromatography (RP TLC) on the silica gel impregnated with paraffin oil stationary phases. Binary mixtures of methanol-water, acetone-water and dioxane-water were used as mobile phases. Retention factors, RM0, corresponding to zero percent organic modifier in the aqueous mobile phase was determined. Lipophilicity C0 was calculated as the ratio of the intercept and slope values. There was satisfactory correlation between them and log P values calculated using different theoretical procedures. Some of these correlations offer very good predicting models, which are important for a better understanding of the relationships between chemical structure and retention. The study showed that the hydrophobic parameters RM0 and C0 can be used as a measures of lipophilicity of investigated compounds.

scholarly journals Thin-layer chromatography of L-carnitine in micellar and cyclodextrin mobile phases

2019 ◽  
Vol 58 (5) ◽  
pp. 62-69
Author(s):  
Anna O. Ustyugova ◽  
◽  
Elena G. Sumina ◽  
Varsenia Z. Uglanova ◽  
Olga N. Novozhilova ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Separation Efficiency ◽  
Sodium Dodecyl ◽  
Theoretical Plate ◽  
Process Efficiency ◽  
Chromatographic Behavior ◽  
Mobile Phases ◽  
Wide Range ◽  
Layer Chromatography

L-carnitine (lat. Levocarnitinum, eng. Levocarnitine) is a natural substance akin to B vitamins. Normalizing metabolic processes in the human body and having a wide range of therapeutic actions, L-carnitine is widely used in cardiology, neurology, gastroenterology, and sports medicine. Currently, modern analytical techniques, such as mass spectrometry, high performance liquid chromatography, radiation methods, etc., are used to analyze L-carnitine. Most of them are rather expensive and time consuming. Thin-layer chromatography (TLC), which is notable for its simplicity, feasibility of analysis, separation efficiency and low cost, has received much less use. In this regard, the purpose of this work was to reveal the analytical capabilities of water-organic, micellar and cyclodextrin mobile phases for the analysis of L-carnitine by means of TLC. Initial L-carnitine solutions with a concentration of 20 mg/ml were prepared by diluting solutions for injections. Their chromatography was carried out by ascending thin-layer chromatography on plates of various polarities (Sorbfil, Plasmachrom, and RP-18) with a fixed sorbent layer. Aqueous solutions of surfactants (cetyltrimethylammonium bromide, cetylpyridinium chloride, sodium dodecyl sulfate, Triton X-100), of cyclodextrins (-cyclodextrin, 2-hydroxypropyl--cyclodextrin), hydroxypropyl--cyclodextrin) were used as mobile phases. The zone of the main substance (L-carnitine) was identified by treating the plate with a mixture of freshly prepared 0.1N solution of potassium permanganate and 2.0 N acetic acid (the zone was colored in a light yellow color). Main features of the chromatographic behavior of L-carnitine were revealed. Based on the calculation of the number of theoretical plates and the height equivalent to one theoretical plate, it was shown that aqueous micellar and cyclodextrin mobile phases allow one to improve the chromatographic process efficiency and the shape of the chromatographic zones of L-carnitine as compared to water-organic eluents; cheaper Sorbfil plates (Russia) can be used for practical purposes. Optimal chromatographic systems and conditions for their use in the thin-layer chromatography of pharmaceuticals and food products have been selected and substantiated.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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