scholarly journals Synthetic Studies on Phosphorylating Reagent. IV. A Novel Synthesis of Nucleoside-3′,5′ Cyclic Phosphates by the Use of 2-(N,N-Dimethylamino)-4-nitrophenyl Phosphate

1975 ◽  
Vol 48 (5) ◽  
pp. 1528-1532 ◽  
Author(s):  
Yoshihiko Taguchi ◽  
Yoshitaka Mushika
Keyword(s):  
Novel Synthesis ◽  
Nitrophenyl Phosphate ◽  
Synthetic Studies ◽  
Cyclic Phosphates

LINGCOD MUSCLE NUCLEASE

1959 ◽  
Vol 37 (8) ◽  
pp. 945-952 ◽  
Author(s):  
Neil Tomlinson
Keyword(s):  
Ribonucleic Acid ◽  
Free Base ◽  
Ophiodon Elongatus ◽  
Base Form ◽  
Nitrophenyl Phosphate ◽  
Diethylaminoethyl Cellulose ◽  
Cyclic Phosphates ◽  
Low Rate

A nuclease from muscle of lingcod (Ophiodon elongatus) has been purified by chromatography on diethylaminoethyl cellulose in the free-base form by stepwise elution with increasing concentrations of tris(hydroxymethyl)aminomethane. HCl, pH 7.0. The nuclease has been shown to hydrolyze ribonucleic acid, adenosine 3′-benzyl phosphate, thymidine 3′-p-nitrophenyl phosphate, and thymidine 5′-p-nitrophenyl phosphate. The latter compound was hydrolyzed at a very low rate. It did not hydrolyze adenosine 2′- or 5′- benzyl phosphate or certain nucleoside cyclic phosphates. The enzyme was inhibited by monoiodoacetate but not by heparin.

LINGCOD MUSCLE NUCLEASE

1959 ◽  
Vol 37 (1) ◽  
pp. 945-952 ◽  
Author(s):  
Neil Tomlinson
Keyword(s):  
Ribonucleic Acid ◽  
Free Base ◽  
Ophiodon Elongatus ◽  
Base Form ◽  
Nitrophenyl Phosphate ◽  
Diethylaminoethyl Cellulose ◽  
Cyclic Phosphates ◽  
Low Rate

A nuclease from muscle of lingcod (Ophiodon elongatus) has been purified by chromatography on diethylaminoethyl cellulose in the free-base form by stepwise elution with increasing concentrations of tris(hydroxymethyl)aminomethane. HCl, pH 7.0. The nuclease has been shown to hydrolyze ribonucleic acid, adenosine 3′-benzyl phosphate, thymidine 3′-p-nitrophenyl phosphate, and thymidine 5′-p-nitrophenyl phosphate. The latter compound was hydrolyzed at a very low rate. It did not hydrolyze adenosine 2′- or 5′- benzyl phosphate or certain nucleoside cyclic phosphates. The enzyme was inhibited by monoiodoacetate but not by heparin.

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