scholarly journals Theoretical Studies of Light Scattering by Polydispersed Colloidal Systems of Spherical Particles

1964 ◽  
Vol 37 (11) ◽  
pp. 1634-1640 ◽  
Author(s):  
Masayuki Nakagaki ◽  
Tsugiyo Shimoyama
Keyword(s):  
Light Scattering ◽  
Spherical Particles ◽  
Theoretical Studies ◽  
Colloidal Systems

scholarly journals Laboratory Measurements of Light Scattering by Dust Particles

1996 ◽  
Vol 150 ◽  
pp. 409-413
Author(s):  
Patrick P. Combet ◽  
Philippe L. Lamy
Keyword(s):  
Light Scattering ◽  
Laser Beam ◽  
Mie Theory ◽  
Spherical Particles ◽  
Dust Particles ◽  
Laboratory Measurements ◽  
Scattering Function ◽  
Hene Laser ◽  
Set Up ◽  
Good Agreement

AbstractWe have set up an experimental device to optically study the scattering properties of dust particles. Measurements over the 8 — 174° interval of scattering angles are performed on a continuously flowing dust loaded jet illuminated by a polarized red HeNe laser beam. The scattering is averaged over the population of the dust particles in the jet, which can be determined independently, and give the “volume scattering function” for the two directions of polarization directly. While results for spherical particles are in good agreement with Mie theory, those for arbitrary particles show conspicuous deviations.

Numerical Simulation and Experimental Study on Light Scattering by Biological Cells with Discrete Dipole Approximation

2013 ◽  
Vol 760-762 ◽  
pp. 105-109 ◽  
Author(s):  
Jian Bin Liu ◽  
Hai Li ◽  
Ying Xin Zeng ◽  
Jia Wen Weng ◽  
Chu Ping Yang
Keyword(s):  
Cell Culture ◽  
Light Scattering ◽  
Culture Medium ◽  
Dipole Approximation ◽  
Spherical Particles ◽  
Biological Cells ◽  
Cell Culture Medium ◽  
Animal Cells ◽  
Experimental Apparatus ◽  
Liquid Suspensions

An experimental apparatus for the analysis of biological cells light scattering in liquid suspensions has been presented. Characterization is based on the scattering of a monochromatic laser beam by particles [which can be inorganic, organic, or biological (such as animal cells and bacteria)] and on the strong relation between the light-scattering pattern and the morphology and refractive index of the particles. In order to study light scattering in biological cells close to the actual situation, we focus on non-spherical particles in the cell-culture medium. Finally, we demonstrate the light scattering results of bovine kidney cells suspended in the cell-culture medium, and compares then with the simulated results.

scholarly journals Application of Dynamic and Static Light Scattering for Size and Shape Characterization of Small Extracellular Nanoparticles in Plasma and Ascites of Ovarian Cancer Patients

2021 ◽  
Vol 22 (23) ◽  
pp. 12946
Author(s):  
Ksenija Kogej ◽  
Darja Božič ◽  
Borut Kobal ◽  
Maruša Herzog ◽  
Katarina Černe
Keyword(s):  
Ovarian Cancer ◽  
Light Scattering ◽  
Spherical Particles ◽  
Radius Of Gyration ◽  
Non Invasive ◽  
Peritoneal Washing ◽  
Angular Dependency ◽  
Shape Characterization ◽  
Further Development

In parallel to medical treatment of ovarian cancer, methods for the early detection of cancer tumors are being sought. In this contribution, the use of non-invasive static (SLS) and dynamic light scattering (DLS) for the characterization of extracellular nanoparticles (ENPs) in body fluids of advanced serous ovarian cancer (OC) and benign gynecological pathology (BP) patients is demonstrated and critically evaluated. Samples of plasma and ascites (OC patients) or plasma, peritoneal fluid, and peritoneal washing (BP patients) were analyzed. The hydrodynamic radius (Rh) and the radius of gyration (Rg) of ENPs were calculated from the angular dependency of LS intensity for two ENP subpopulations. Rh and Rg of the predominant ENP population of OC patients were in the range 20–30 nm (diameter 40–60 nm). In thawed samples, larger particles (Rh mostly above 100 nm) were detected as well. The shape parameter ρ of both particle populations was around 1, which is typical for spherical particles with mass concentrated on the rim, as in vesicles. The Rh and Rg of ENPs in BP patients were larger than in OC patients, with ρ ≈ 1.1–2, implying a more elongated/distorted shape. These results show that SLS and DLS are promising methods for the analysis of morphological features of ENPs and have the potential to discriminate between OC and BP patients. However, further development of the methodology is required.

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